全文获取类型
收费全文 | 389篇 |
免费 | 21篇 |
出版年
2024年 | 1篇 |
2023年 | 8篇 |
2022年 | 7篇 |
2021年 | 19篇 |
2020年 | 9篇 |
2019年 | 9篇 |
2018年 | 16篇 |
2017年 | 17篇 |
2016年 | 12篇 |
2015年 | 21篇 |
2014年 | 19篇 |
2013年 | 31篇 |
2012年 | 40篇 |
2011年 | 48篇 |
2010年 | 24篇 |
2009年 | 15篇 |
2008年 | 19篇 |
2007年 | 24篇 |
2006年 | 14篇 |
2005年 | 14篇 |
2004年 | 12篇 |
2003年 | 8篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1990年 | 2篇 |
1978年 | 2篇 |
1954年 | 1篇 |
1953年 | 2篇 |
排序方式: 共有410条查询结果,搜索用时 15 毫秒
11.
Nguyen Thi Dieu Thuy Nguyen Thi Thu Nguyen Giang Son Le Thi Thu Ha Vo Khanh Hung Nguyen Thao Nguyen Do Vo Anh Khoa 《Microbiology and immunology》2013,57(7):518-526
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important swine pathogens because it is highly infectious and causes economic losses due to decreased pig productivity. In this study, the 603 bp complete major envelope protein encoding gene (ORF5) of 32 field PRRSV isolates from Vietnam collected during 2008–2012 were sequenced and analyzed. Multiple nucleotide (nt) and deduced amino acid (aa) alignments of ORF5 were performed on the 32 isolates: the representative strains (European and North American genotypes), Chinese strains available in GenBank and vaccine strains licensed for use in Vietnam. The results showed 94.8–100.0% nt identity and 94.0–100% aa similarity among the 32 isolates. These isolates shared similarities with the prototype of the North American PRRSV strain (VR‐2332; nt 87.8–89.3%, aa 87.5–90.0%), and Lelystat virus, the prototype of the European PRRSV strain (LV; nt 61.1–61.9%, aa 55.1‐57.0%). There was greater similarity with QN07 (nt 96.5‐98.5%, aa 96.0‐99.0%) from the 2007 PRRS outbreak in QuangNam Province, CH‐1a (nt 93.2–95.1%, 91.5–93.5%) isolated in China in 1995 and JXA1 (nt 96.5–98.6%, aa 95.0–98.0%), the highly pathogenic strain from China isolated in 2006. The Vietnamese isolates were more similar to JXA1‐R (nt 96.5–98.6%, aa 95.0–98.0%), the strain used in Chinese vaccines, than to Ingelvac MLV/BSL‐PS (nt 87.2–89.0%, aa 86.0–89.0%). Phylogenetic analysis showed that the 32 isolates were of the North American genotype and classified into sub‐lineage 8.7. This sub‐lineage contains highly pathogenic Chinese PRRSV strains. This study documents genetic variation in circulating PRRSV strains and could assist more effective use of PRRS vaccines in Vietnam. 相似文献
12.
Nguyen Hoang Loc Le My Tieu Ngoc Tran Thuy Lan Le Quoc Viet Le Duc Thao Hoang Tan Quang Dinh Thi Bich Lan Phung Thang Long 《Indian journal of microbiology》2013,53(4):488-491
We cloned two genes coding F107-C and K88-1NT fimbrial subunits from strains E. coli C and 1NT isolated from Thua Thien Hue province, Vietnam. The mature peptide of faeG gene from strain E. coli 1NT (called faeG-1NT) is 100 % similarity with faeG gene, while the CDS of fedA gene from strain C (called fedA-C) has a similarity of 97 % with the fedA gene. Expression of the faeG-1NT and fedA-C genes in E. coli BL21 Star™ (DE3) produced proteins of ~31 and 22 kDa, respectively. The effect of IPTG concentration on the K88-1NT and F107-C fimbriae production was investigated. The results showed that 0.5 mM IPTG is suitable for higher expression of K88-1NT subunit, while 0.75 mM IPTG strongly stimulated expression of F107-C subunit. The optimal induction time for expression was also examined. Generally, highest expression of K88-1NT subunit occurred after 6 h of induction, while that of F107-C subunit is after 14 h. 相似文献
13.
Using molecular dynamics simulations, we studied the structure, interhelix interactions, and dynamics of transmembrane proteins. Specifically, we investigated homooligomeric helical bundle systems consisting of synthetic α-helices with either the sequence Ac-(LSLLLSL)3-NH2 (LS2) or Ac-(LSSLLSL)3-NH2 (LS3). The LS2 and LS3 helical peptides are designed to have amphipathic characteristics that form ion channels in membrane. We simulated bundles containing one to six peptides that were embedded in palmitoyl-oleoyl-phosphatidylcholine (POPC) lipid bilayer and placed between two lamellae of water. We aim to provide a fundamental understanding of how amphipathic helical peptides interact with each other and their dynamical behaviors in different homooligomeric states. To understand structural properties, we examined the helix lengths, tilt angles of individual helices and the entire bundle, interhelix distances, interhelix cross-angles, helix hydrophobic-to-hydrophilic vector projections, and the average number of interhelix hydrophilic (serine–serine) contacts lining the pore of the transmembrane channel. To analyze dynamical properties, we calculated the rotational autocorrelation function of each helix and the cross-correlation of the rotational velocity between adjacent helices. The observed structural and dynamical characteristics show that higher order bundles containing four to six peptides are composed of multiple lower order bundles of one to three peptides. For example, the LS2 channel was found to be stable in a tetrameric bundle composed of a “dimer of dimers.” In addition, we observed that there is a minimum of two strong hydrophilic contacts between a pair of adjacent helices in the dimer to tetramer systems and only one strong hydrophilic interhelix contact in helix pairs of the pentamer and hexamer systems. We believe these results are general and can be applied to more complex ion channels, providing insight into ion channel stability and assembly. 相似文献
14.
Mark J. Mitton-Fry Steven J. Brickner Judith C. Hamel Lori Brennan Jeffrey M. Casavant Michael Chen Tao Chen Xiaoyuan Ding James Driscoll Joel Hardink Thuy Hoang Erbing Hua Michael D. Huband Meghan Maloney Anthony Marfat Sandra P. McCurdy Dale McLeod Michael Plotkin Christopher Zook 《Bioorganic & medicinal chemistry letters》2013,23(10):2955-2961
A structurally novel set of inhibitors of bacterial type II topoisomerases with potent in vitro and in vivo antibacterial activity was developed. Dual-targeting ability, hERG inhibition, and pharmacokinetic properties were also assessed. 相似文献
15.
Junko Tanuma Kyu Ha Lee Sebastien Haneuse Shoko Matsumoto Dung Thi Nguyen Dung Thi Hoai Nguyen Cuong Duy Do Thuy Thanh Pham Kinh Van Nguyen Shinichi Oka 《PloS one》2016,11(3)
Background
Although the prognosis for HIV-infected individuals has improved after antiretroviral therapy (ART) scale-up, limited data exist on the incidence of AIDS-defining opportunistic infections (ADIs) and mortality during ART in resource-limited settings.Methods
HIV-infected adults in two large hospitals in urban Hanoi were enrolled to the prospective cohort, from October 2007 through December 2013. Those who started ART less than one year before enrollment were assigned to the survival analysis. Data on ART history and ADIs were collected retrospectively at enrollment and followed-up prospectively until April 2014.Results
Of 2,070 cohort participants, 1,197 were eligible for analysis and provided 3,446 person-years (PYs) of being on ART. Overall, 161 ADIs episodes were noted at a median of 3.20 months after ART initiation (range 0.03–75.8) with an incidence 46.7/1,000 PYs (95% confidence interval [CI] 39.8–54.5). The most common ADI was tuberculosis with an incidence of 29.9/1,000 PYs. Mortality after ART initiation was 8.68/1,000 PYs and 45% (19/45) died of AIDS-related illnesses. Age over 50 years at ART initiation was significantly associated with shorter survival after controlling for baseline CD4 count, but neither having injection drug use (IDU) history nor previous ADIs were associated with poor survival. Semi-competing risks analysis in 951 patients without ADIs history prior to ART showed those who developed ADIs after starting ART were at higher risk of death in the first six months than after six months.Conclusion
ADIs were not rare in spite of being on effective ART. Age over 50 years, but not IDU history, was associated with shorter survival in the cohort. This study provides in-depth data on the prognosis of patients on ART in Vietnam during the first decade of ART scale-up. 相似文献16.
Won‐Jun Jang Sung Keun Jung Tam Thuy Lu Vo Chul‐Ho Jeong 《Journal of cellular and molecular medicine》2019,23(2):1106-1115
The concept of drug repositioning has recently received considerable attention in the field of oncology. In the present study, we propose that paroxetine can be used as a potent anticancer drug. Paroxetine, one of the selective serotonin reuptake inhibitors (SSRIs), has been widely prescribed for the treatment of depression and anxiety disorders. Recently, SSRIs have been reported to have anticancer activity in various types of cancer cells; however, the underlying mechanisms of their action are not yet known. In this study, we investigated the potential anticancer effect of paroxetine in human colorectal cancer cells, HCT116 and HT‐29. Treatment with paroxetine reduced cell viability, which was associated with marked increase in apoptosis, in both the cell lines. Also, paroxetine effectively inhibited colony formation and 3D spheroid formation. We speculated that the mode of action of paroxetine might be through the inhibition of two major receptor tyrosine kinases – MET and ERBB3 – leading to the suppression of AKT, ERK and p38 activation and induction of JNK and caspase‐3 pathways. Moreover, in vivo experiments revealed that treatment of athymic nude mice bearing HT‐29 cells with paroxetine remarkably suppressed tumour growth. In conclusion, paroxetine is a potential therapeutic option for patients with colorectal cancer. 相似文献
17.
Tran Cuong Manh Van Pham Hai Nguyen Hien Thuy Nguyen Thuy Thi Vu Lam Dinh Do Tung Hoang 《Plasmonics (Norwell, Mass.)》2019,14(6):1587-1592
Plasmonics - Since the metamaterial perfect absorber (MPA) is composed of electromagnetic resonance structures, the main operational mechanism of the MPA is electromagnetic resonance. In this work,... 相似文献
18.
Duc Khanh Le Quang Khai Le Thi Thuy Hang Tran Duc Viet Nguyen Thi Hang Dao Thi Thuy Nguyen Xuan Lam Truong Quang Cuong Nguyen Huy Phong Pham Thi Thu Thuy Phan Gajendra Babu Baktavachalam 《Journal of Asia》2019,22(2):493-498
Susceptibility of Asian corn borer (ACB), Ostrinia furnacalis (Guenée) to Bacillus thuringiensis (Bt) Cry1Ab protein was studied between 2015 and 2016 with 11 ACB populations, collected from various geographical regions in Vietnam. A concentration range of Cry1Ab from 0.20 to 26.10 ng/cm2 of diet was evaluated against F1 ACB neonates using diet surface-overlay bioassays. Mortality data was recorded daily until seven days after infestation. Growth inhibition was recorded at the end of seven days. The median lethal concentration (LC50) varied ≈3-fold among the different populations, ranging from 0.58 to 1.83 ng/cm2 of diet with an overall mean of 0.86 ng/cm2 of diet. Even the lowest concentration of 0.20 ng/cm2 caused 73.53% growth inhibition. >90% growth inhibition was achieved at 0.82 ng/cm2 or higher concentrations. The results reflect natural variation in Bt susceptibility among ACB populations rather than variation caused by prior exposure to selection pressures. LC99 value (17.26 ng/cm2) was generated by pooling mortality data across different populations. The upper fiducial limit of LC99 (24.38 ng/cm2) could be a potential diagnostic dose for future resistance monitoring programs. The findings from this study suggest that ACB populations in Vietnam are highly susceptible to Cry1Ab protein. This is the first report of Cry1Ab susceptibility of different ACB populations in Vietnam and will serve as a baseline for future resistance monitoring work. 相似文献
19.
20.
Hinney A Nguyen TT Scherag A Friedel S Brönner G Müller TD Grallert H Illig T Wichmann HE Rief W Schäfer H Hebebrand J 《PloS one》2007,2(12):e1361