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971.
Rmg8 and Rmg7, wheat genes for resistance to the wheat blast fungus,recognize the same avirulence gene AVR‐Rmg8 下载免费PDF全文
Vu Lan Anh Yoshihiro Inoue Soichiro Asuke Trinh Thi Phuong Vy Nguyen Tuan Anh Shizhen Wang Izumi Chuma Yukio Tosa 《Molecular Plant Pathology》2018,19(5):1252-1256
Rmg8 and Rmg7 are genes for resistance to the wheat blast fungus (Pyricularia oryzae), located on chromosome 2B in hexaploid wheat and chromosome 2A in tetraploid wheat, respectively. AVR‐Rmg8, an avirulence gene corresponding to Rmg8, was isolated from a wheat blast isolate through a map‐based strategy. The cloned fragment encoded a small protein containing a putative signal peptide. AVR‐Rmg8 was recognized not only by Rmg8, but also by Rmg7, suggesting that these two resistance genes are equivalent to a single gene from the viewpoint of resistance breeding. 相似文献
972.
Toll‐like receptors 2 and 3 enhance melanogenesis and melanosome transport in human melanocytes 下载免费PDF全文
Saaya Koike Kenshi Yamasaki Takeshi Yamauchi Mai Inoue Ryoko Shimada‐Ohmori Kenichiro Tsuchiyama Setsuya Aiba 《Pigment cell & melanoma research》2018,31(5):570-584
Because little is known about how the innate immune response influences skin pigmentation, we examined whether Toll‐like receptor (TLR) agonists participate in melanogenesis and melanosome transportation. We observed that TLR2/2 agonist HKLM and TLR3 agonist Poly(I:C) increased the amount of extracellular melanin from primary human epidermal melanocytes. HKLM, but not Poly(I:C), increased the melanogenic genes such as tyrosinase and dopachrome tautomerase. Poly(I:C) increased the expression of Rab27A, a molecule that facilitates melanosome transport to perimembranous actin filament. UVB irradiation induced Rab27A and melanosome transportation in a similar manner of Poly(I:C). SiRNA for TLR3 or Rab27A suppressed the perimembranous accumulation of Gp100‐positive vesicles in melanocytes and decreased melanin transfer to neighboring keratinocytes induced by both Poly(I:C) and UVB. These results suggest that the microenvironment in the epidermis and innate immune stimuli, such as microbiome and ultraviolet represented here by TLR2 and TLR3 agonists, could affect the melanogenesis in human melanocytes. 相似文献
973.
Kazutaka Miyamoto Mizuha Akiyama Fumiya Tamura Mari Isomi Hiroyuki Yamakawa Taketaro Sadahiro Naoto Muraoka Hidenori Kojima Sho Haginiwa Shota Kurotsu Hidenori Tani Li Wang Li Qian Makoto Inoue Yoshinori Ide Junko Kurokawa Tsunehisa Yamamoto Tomohisa Seki Masaki Ieda 《Cell Stem Cell》2018,22(1):91-103.e5
974.
Differential expression of a fructose receptor gene in honey bee workers according to age and behavioral role 下载免费PDF全文
Tomoyuki Takada Taiyo Sasaki Ryoichi Sato Shingo Kikuta Maki N. Inoue 《Archives of insect biochemistry and physiology》2018,97(2)
Honey bee (Apis mellifera) workers contribute to the maintenance of colonies in various ways. The primary functions of workers are divided into two types depending on age: young workers (nurses) primarily engage in such behaviors as cleaning and food handling within the hive, whereas older workers (foragers) acquire floral nutrients beyond the colony. Concomitant with this age‐dependent change in activity, physiological changes occur in the tissues and organs of workers. Nurses supply younger larvae with honey containing high levels of glucose and supply older larvae with honey containing high levels of fructose. Given that nurses must determine both the concentration and type of sugar used in honey, gustatory receptors (Gr) expressed in the chemosensory organs likely play a role in distinguishing between sugars. Glucose is recognized by Gr1 in honey bees (AmGr1); however, it remains unclear which Gr are responsible for fructose recognition. This study aimed to identify fructose receptors in honey bees and reported that AmGr3, when transiently expressed in Xenopus oocytes, responded only to fructose, and to no other sugars. We analyzed expression levels of AmGr3 to identify which tissues and organs of workers are involved in fructose recognition and determined that expression of AmGr3 was particularly high in the antennae and legs of nurses. Our results suggest that nurses use their antennae and legs to recognize fructose, and that AmGr3 functions as an accurate nutrient sensor used to maintain food quality in honey bee hives. 相似文献
975.
Mizue Inoue Hiroshi Nakada Yoshifumi Oka Nobuhiro Tanaka Ikuo Yamashina 《Glycoconjugate journal》1997,14(1):147-153
Novel glycoproteins carrying sialyl-LeA (SLeA) antigens (SL-GP) were isolated from ascites fluid from a patient with colorectal cancer by immunoaffinity chromatography. Their characteristics, including binding capacity to E-selectin, were investigated. SL-GP showed a typical mucin type amino acid composition in which Ser, Thr and Pro together accounted for greater than 50% of the total amino acid residues. A large amount of carbohydrate (about 80%) was present in SL-GP. The number of O-glycans carrying SLeA antigens comprised about 9% of the total number of O-glycosidic chains. SL-GP could bind to IL-1 treated HUVEC, and the binding was inhibited by anti-E-selectin and anti-SLeA monoclonal antibodies. The binding of colorectal cancer cells, LS 180, to HUVEC was assayed in the presence of SL-GP, oligosaccharides prepared from SL-GP and human milk SLeA hexasaccharide. SL-GP inhibited the binding most effectively, whereas equivalent amounts of the SL-GP oligosaccharides and milk SLeA hexasaccharide inhibited it only slightly. These results constitute direct evidence that a unique arrangement of SLeA antigens on the polypeptide chain, probably a cluster, is essential for the binding to E-selectin. 相似文献
976.
977.
978.
Tetsushi Azuma Satoshi Ueno Naotsugu Uchida Takeshi Yasuda 《Physiologia plantarum》1997,99(4):517-522
Internodal elongation in floating rice ( Oryza sativa L. cv. Habiganj Aman II) is known to be enhanced by treatment with ethylene or gibberellic acid (GA3 ) at high relative humidity (RH). However, ethylene-induced internodal elongation is inhibited at low RH. while GA3 -induced internodal elongation is hardly affected by humidity. We examined the possible involvement of osmoregulation in the stimulation by GA3 of the elongation of internodes at low RH. Submergence and treatment with ethylene or GA33 at 100% RH increased the osmotic potential in internodes of excised stem segments, while GA3 at 20% RH maintained the osmotic potential at a low level. In internodes of stem segments that had been treated with GA3 at 20% RH, the activity of invertase and the level of soluble sugars were almost 2- and 1.5-fold higher, respectively, than those in internodes that had been treated with GA3 at 100% RH. These results indicate that one of the possible mechanisms by which GA3 promotes elongation of internodes at low RH involves the osmoregulation that is achieved by promotion of the synthesis of invertase. 相似文献
979.
A sensitive and efficient method to analyze oligo/poly-sialic acids containing α2–8-linked 5-N-acetylneuraminic acid (Neu5Ac), 5-N-glycolylneuraminic acid (Neu5Gc), and deaminated neuraminic acid (KDN) using high-performance anion-exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD-2) has been developed. Using a CarboPac PA-100 column and sodium nitrate as the pushing agent, polymers in colominic acid with degree of polymerization (DP) up to 80 were separated in 68 min. A similar DP-based resolution was also obtained on a CarboPac PA-1 column. The elution ladders of the Neu5Ac, Neu5Gc, and KDN series were sufficiently different to be used as diagnostic indices. This technique was applied to identification of the sialic acid components in a polysialoglycoprotein (PSGP) sample as well as monitoring the oligo/poly-KDN-containing fractions during the purification of KDN-containing glycoprotein (KDN-gp). The maximum DPs of oligo-Neu5Gc and oligo-KDN that can be detected in PSGP and KDN-gp hydrolysates were 11 and 8, respectively. The high sensitivity of this method was demonstrated by the quantification of Neu5Ac oligomers. Distributions of the monomer and oligo/polymers in the acid and enzymatic hydrolysates of colominic acid and PSGP under different conditions were also studied. 相似文献
980.
Noguchi Takumi; Kamimura Yasumaro; Inoue Yorinao; Itoh Shigeru 《Plant & cell physiology》1999,40(3):305-310
A water-soluble Chl a/b-protein complex, CP668, from Chenopodiumalbum converts to another form of protein complex, CP743, uponlight illumination. Structural changes of pigments and proteinsupon photoconversion were studied using resonance Raman (RR)and Fourier transform infrared (FTIR) spectroscopies. RR spectraof CP668 and CP743 and a light-induced FTIR difference spectrumshowed that the macrocyle C=C bands of Chl a in CP668 considerablychanged upon conversion to the pigment (not chemically identifiedyet) in CP743. The C=C band pattern of the RR spectrum of CP743was similar to that of bacteriochlorophyll a, suggesting thatthe conjugated system of the CP743 pigment resembles a bacteriochlorinring. Judging from the C=O frequencies, the 131-keto C=O groupsof Chl a and b in CP668 are free from hydrogen bonding, whereasthe 132-ester C=O groups of both Chl a and b and the 7-formylC=O of Chl b in CP668 are hydrogen bonded. Upon conversion toCP743, interactions of the 131-keto and 132-ester C=O groupswere basically unaffected, demonstrating no drastic changesaround these C=O groups. FTIR spectra in the amide I' regionof CP668 and CP743 in D2O buffer showed a peak at 1,633 cm1,which represents a major component of ß-sheet conformation.Second-derivative spectra of the amide I' bands as well as alight-induced FTIR difference spectrum suggested that drasticchange in the protein conformation does not occur upon photoconversion. (Received November 1, 1998; Accepted December 24, 1998) 相似文献