全文获取类型
收费全文 | 494篇 |
免费 | 54篇 |
出版年
2023年 | 3篇 |
2021年 | 6篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2016年 | 14篇 |
2015年 | 10篇 |
2014年 | 15篇 |
2013年 | 15篇 |
2012年 | 21篇 |
2011年 | 28篇 |
2010年 | 17篇 |
2009年 | 17篇 |
2008年 | 21篇 |
2007年 | 26篇 |
2006年 | 17篇 |
2005年 | 25篇 |
2004年 | 18篇 |
2003年 | 9篇 |
2002年 | 18篇 |
2001年 | 11篇 |
2000年 | 17篇 |
1999年 | 9篇 |
1998年 | 11篇 |
1997年 | 8篇 |
1996年 | 12篇 |
1995年 | 8篇 |
1994年 | 5篇 |
1993年 | 7篇 |
1992年 | 16篇 |
1991年 | 14篇 |
1990年 | 12篇 |
1989年 | 12篇 |
1988年 | 17篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 11篇 |
1984年 | 13篇 |
1983年 | 3篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1973年 | 3篇 |
1969年 | 3篇 |
1968年 | 2篇 |
1967年 | 3篇 |
排序方式: 共有548条查询结果,搜索用时 78 毫秒
41.
Abete P Testa G Ferrara N De Santis D Capaccio P Viati L Calabrese C Cacciatore F Longobardi G Condorelli M Napoli C Rengo F 《American journal of physiology. Heart and circulatory physiology》2002,282(6):H1978-H1987
Ischemic preconditioning (PC) has been proposed as an endogenous form of protection against-ischemia reperfusion injury. We have shown that PC does not prevent postischemic dysfunction in the aging heart. This phenomenon could be due to the reduction of cardiac norepinephrine release, and it has also been previously demonstrated that age-related decrease of norepinephrine release from cardiac adrenergic nerves may be restored by caloric restriction. We investigated the effects on mechanical parameters of PC against 20 min of global ischemia followed by 40 min of reperfusion in isolated hearts from adult (6 mo) and "ad libitum"-fed and food-restricted senescent (24 mo) rats. Norepinephrine release in coronary effluent was determined by high-performance liquid chromatography. Final recovery of percent developed pressure was significantly improved after PC in adult hearts versus unconditioned controls (85.2 +/- 19% vs. 51.5 +/- 10%, P < 0.01). The effect of PC on developed pressure recovery was absent in ad libitum-fed rats, but it was restored in food-restricted senescent hearts (66.6 +/- 13% vs. 38.3 +/- 11%, P < 0.05). Accordingly, norepinephrine release significantly increased after PC in both adult and in food-restricted senescent hearts, and depletion of myocardial norepinephrine stores by reserpine abolished the PC effect in both adult and in food-restricted senescent hearts. We conclude that PC reduces postischemic dysfunction in the hearts from adult and food-restricted but not in ad libitum-fed senescent rats. Despite the possibility of multiple age-related mechanisms, the protection afforded by PC was correlated with increased norepinephrine release, and it was blocked by reserpine in both adult and food-restricted senescent hearts. Thus caloric restriction may restore PC in the aging heart probably via increased norepinephrine release. 相似文献
42.
Park B Nguyen NT Dutt P Merdek KD Bashar M Sterpetti P Tosolini A Testa JR Toksoz D 《The Journal of biological chemistry》2002,277(47):45361-45370
43.
Czene S Testa E Nygren J Belyaev I Harms-Ringdahl M 《Biochemical and biophysical research communications》2002,294(4):872-878
Cell suspensions enriched in cells at various stages of apoptosis were obtained by separation of irradiated human peripheral blood lymphocytes on density gradients at different post-irradiation times. The state of DNA fragmentation in the cells was determined by comet assay and pulsed field gel electrophoresis. The morphologically distinguishable features of apoptosis such as chromatin condensation and cell shrinkage correlated with discrete stages of DNA fragmentation. It was found that >/=50 kbp fragmentation of DNA occurs already in cells of normal density whereas the subsequent DNA fragmentation onto fragments <50 kbp occurs in parallel with cell shrinkage and simultaneous increase in cell density.The observed stages of DNA fragmentation seem to be separated in time that could allow in case of abortive apoptosis formation of chromosomal aberrations. 相似文献
44.
45.
46.
47.
Previously, a rodent cDNA encoding the third member of the Akt/PKB family of serine/threonine kinases was cloned. We have now cloned the human homolog of this cDNA, and we have used this clone to map the AKT3 gene to human chromosome 1q44 by fluorescence in situ hybridization (FISH). We have also mapped the rodent homologs of AKT3 to rat chromosome 13q24-->q26 and mouse chromosome 1H4-6 by FISH. 相似文献
48.
OCI-5/GPC3, a Glypican Encoded by a Gene That Is Mutated in the Simpson-Golabi-Behmel Overgrowth Syndrome, Induces Apoptosis in a Cell Line–specific Manner 下载免费PDF全文
Alfonso Dueas Gonzalez Mitsunori Kaya Wen Shi Howard Song Joseph R. Testa Linda Z. Penn Jorge Filmus 《The Journal of cell biology》1998,141(6):1407-1414
OCI-5/GPC3 is a member of the glypican family. Glypicans are heparan sulfate proteoglycans that are bound to the cell surface through a glycosyl-phosphatidylinositol anchor. It has recently been shown that the OCI-5/GPC3 gene is mutated in patients with the Simpson-Golabi-Behmel Syndrome (SGBS), an X-linked disorder characterized by pre- and postnatal overgrowth and various visceral and skeletal dysmorphisms. Some of these dysmorphisms could be the result of deficient growth inhibition or apoptosis in certain cell types during development. Here we present evidence indicating that OCI-5/GPC3 induces apoptosis in cell lines derived from mesothelioma (II14) and breast cancer (MCF-7). This induction, however, is cell line specific since it is not observed in NIH 3T3 fibroblasts or HT-29 colorectal tumor cells. We also show that the apoptosis-inducing activity in II14 and MCF-7 cells requires the anchoring of OCI-5/GPC3 to the cell membrane. The glycosaminoglycan chains, on the other hand, are not required. MCF-7 cells can be rescued from OCI-5/GPC3–induced cell death by insulin-like growth factor 2. This factor has been implicated in Beckwith-Wiedemann, an overgrowth syndrome that has many similarities with SGBS. The discovery that OCI-5/GPC3 is able to induce apoptosis in a cell line– specific manner provides an insight into the mechanism that, at least in part, is responsible for the phenotype of SGBS patients. 相似文献
49.
50.
Magda Gioia Giulia Vindigni Barbara Testa Sofia Raniolo Giovanni Francesco Fasciglione Massimiliano Coletta Silvia Biocca 《PloS one》2015,10(10)
The lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a scavenger receptor responsible for ox-LDL recognition, binding and internalization, which is up-regulated during atherogenesis. Its activation triggers endothelium dysfunction and induces inflammation. A soluble form of LOX-1 has been identified in the human blood and its presence considered a biomarker of cardiovascular diseases. We recently showed that cholesterol-lowering drugs inhibit ox-LDL binding and internalization, rescuing the ox-LDL induced apoptotic phenotype in primary endothelial cells. Here we have investigated the molecular bases of human LOX-1 shedding by metalloproteinases and the role of cell membrane cholesterol on the regulation of this event by modulating its level with MβCD and statins. We report that membrane cholesterol affects the release of different forms of LOX-1 in cells transiently and stably expressing human LOX-1 and in a human endothelial cell line (EA.hy926). In particular, our data show that i) cholesterol depletion triggers the release of LOX-1 in exosomes as a full-length transmembrane isoform and as a truncated ectodomain soluble fragment (sLOX-1); ii) endothelial cells secrete a soluble metalloproteinase which induces LOX-1 ectodomain shedding and iii) long term statins treatment enhances sLOX-1 proteolytic shedding. 相似文献