Genetic composition of the Jadro softmouth trout following translocation into a new habitat

Genetic founder effects of a historical translocation (1965; app. 12 generations ago) of endangered softmouth trout from the only remaining indigenous population to an adjacent uninhabited river were investigated. A comparison of 15 microsatellites from contemporary samples in both the source and re-established populations indicated a very low level of genetic diversity within the two populations. Furthermore, considerable differences in estimates of variability and effective population sizes were detected between populations, but no recent bottlenecks were evident. Our data suggest that the re-established population has lost variation due to genetic drift associated with founder effects following the translocation. Accordingly, in relation to management, we suggest that the re-established population could serve as a secondary source of individuals to buffer possible extinction due to demographic events. Finally, conservation initiatives to enhance the census population size and increase levels of variability in the re-established population are proposed. Electronic Supplementary Material Supplementary material is available in the online version of this article at and is accessible for authorized users.     

Application of molecular tools in detecting zoonotic pathogens in organic fertilizers and liquid supplements

The use of organic fertilizers and liquid supplements for crop production is rapidly growing as an alternative system to conventional agriculture. However, very little is known about the public health issues related to pathogens. This study endeavors to identify the important zoonotic pathogens with the current molecular diagnostic tools, Loop-mediated isothermal amplification (LAMP) and Recombinase polymerase amplification (RPA), against the conventional pathogen detection. These cost-effective molecular techniques have proven to be confirmatory tests of the target pathogens present in organic fertilizers and liquid supplements, which recommends an advancement for the comprehensive field surveillance-response approach in many developing countries with resource-limited settings quality assurance and safety implementation of organic biosolids for sustainable agricultural farming.