全文获取类型
收费全文 | 1272篇 |
免费 | 99篇 |
出版年
2023年 | 3篇 |
2022年 | 12篇 |
2021年 | 26篇 |
2020年 | 21篇 |
2019年 | 19篇 |
2018年 | 28篇 |
2017年 | 28篇 |
2016年 | 46篇 |
2015年 | 48篇 |
2014年 | 59篇 |
2013年 | 72篇 |
2012年 | 105篇 |
2011年 | 108篇 |
2010年 | 69篇 |
2009年 | 54篇 |
2008年 | 106篇 |
2007年 | 103篇 |
2006年 | 94篇 |
2005年 | 70篇 |
2004年 | 87篇 |
2003年 | 69篇 |
2002年 | 49篇 |
2001年 | 4篇 |
2000年 | 6篇 |
1999年 | 8篇 |
1998年 | 14篇 |
1997年 | 5篇 |
1996年 | 6篇 |
1995年 | 5篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 6篇 |
1988年 | 3篇 |
1986年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1969年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有1371条查询结果,搜索用时 15 毫秒
91.
Nikita A. Kuznetsov Nicolai G. Faleev Alexandra A. Kuznetsova Elena A. Morozova Svetlana V. Revtovich Natalya V. Anufrieva Alexei D. Nikulin Olga S. Fedorova Tatyana V. Demidkina 《The Journal of biological chemistry》2015,290(1):671-681
Methionine γ-lyase (MGL) catalyzes the γ-elimination of l-methionine and its derivatives as well as the β-elimination of l-cysteine and its analogs. These reactions yield α-keto acids and thiols. The mechanism of chemical conversion of amino acids includes numerous reaction intermediates. The detailed analysis of MGL interaction with glycine, l-alanine, l-norvaline, and l-cycloserine was performed by pre-steady-state stopped-flow kinetics. The structure of side chains of the amino acids is important both for their binding with enzyme and for the stability of the external aldimine and ketimine intermediates. X-ray structure of the MGL·l-cycloserine complex has been solved at 1.6 Å resolution. The structure models the ketimine intermediate of physiological reaction. The results elucidate the mechanisms of the intermediate interconversion at the stages of external aldimine and ketimine formation. 相似文献
92.
93.
Michael Lauck Sergey V. Alkhovsky Yīmíng Bào Adam L. Bailey Zinaida V. Shevtsova Alexey M. Shchetinin Tatyana V. Vishnevskaya Matthew G. Lackemeyer Elena Postnikova Steven Mazur Jiro Wada Sheli R. Radoshitzky Thomas C. Friedrich Boris A. Lapin Petr G. Deriabin Peter B. Jahrling Tony L. Goldberg David H. O'Connor Jens H. Kuhn 《Journal of virology》2015,89(15):8082-8087
Simian hemorrhagic fever (SHF) is lethal for macaques. Based on clinical presentation and serological diagnosis, all reported SHF outbreaks were thought to be caused by different strains of the same virus, simian hemorrhagic fever virus (SHFV; Arteriviridae). Here we show that the SHF outbreaks in Sukhumi in 1964 and in Alamogordo in 1989 were caused not by SHFV but by two novel divergent arteriviruses. Our results indicate that multiple divergent simian arteriviruses can cause SHF. 相似文献
94.
Dionne C.G. Klein Astrid Skjesol Esther D. Kers‐Rebel Tatyana Sherstova Bjrnar Sporsheim Kjartan W. Egeberg Bjrn T. Stokke Terje Espevik Harald Husebye 《Traffic (Copenhagen, Denmark)》2015,16(7):677-690
Toll‐like receptor 4 (TLR4) is responsible for the immediate response to Gram‐negative bacteria and signals via two main pathways by recruitment of distinct pairs of adaptor proteins. Mal‐MyD88 [Mal (MyD88‐adaptor‐like) ‐ MYD88 (Myeloid differentiation primary response gene (88))] is recruited to the plasma membrane to initiate the signaling cascade leading to production of pro‐inflammatory cytokines while TRAM‐TRIF [TRAM (TRIF‐related adaptor molecule)‐TRIF (TIR‐domain‐containing adapter‐inducing interferon‐β)] is recruited to early endosomes to initiate the subsequent production of type I interferons. We have investigated the dynamics of TLR4 and TRAM during lipopolysaccharide (LPS) stimulation. We found that LPS induced a CD14‐dependent immobile fraction of TLR4 in the plasma membrane. Total internal reflection fluorescence microscopy (TIRF) revealed that LPS stimulation induced clustering of TLR4 into small punctate structures in the plasma membrane containing CD14/LPS and clathrin, both in HEK293 cells and the macrophage model cell line U373‐CD14. These results suggest that laterally immobilized TLR4 receptor complexes are being formed and prepared for endocytosis. RAB11A was found to be involved in localizing TRAM to the endocytic recycling compartment (ERC) and to early sorting endosomes. Moreover, CD14/LPS but not TRAM was immobilized on RAB11A‐positive endosomes, which indicates that TRAM and CD14/LPS can independently be recruited to endosomes. 相似文献
95.
Wu SC Yoon D Chin J van Kirk K Seethala R Golla R He B Harrity T Kunselman LK Morgan NN Ponticiello RP Taylor JR Zebo R Harper TW Li W Wang M Zhang L Sleczka BG Nayeem A Sheriff S Camac DM Morin PE Everlof JG Li YX Ferraro CA Kieltyka K Shou W Vath MB Zvyaga TA Gordon DA Robl JA 《Bioorganic & medicinal chemistry letters》2011,21(22):6693-6698
Derived from the HTS hit 1, a series of hydroxyisoquinolines was discovered as potent and selective 11β-HSD1 inhibitors with good cross species activity. Optimization of substituents at the 1 and 4 positions of the isoquinoline group in addition to the core modifications, with a special focus on enhancing metabolic stability and aqueous solubility, resulted in the identification of several compounds as potent advanced leads. 相似文献
96.
97.
Olga A. Shulga Tatyana Yu Mitiouchkina Anna V. Shchennikova Konstantin G. Skryabin Sergey V. Dolgov 《In vitro cellular & developmental biology. Plant》2011,47(5):553-560
Chrysanthemum is one of the most important commercial cut flowers in the world. Early-flowering cultivars are required to
produce quality chrysanthemum flowers with a lower cost of production. To shorten the vegetative growth phase of chrysanthemum,
three AP1-like genes from Asteraceae were constitutively overexpressed in 80 independent transgenic chrysanthemum lines. All lines were characterized by PCR and
RT-PCR and demonstrated that overexpression of compositae AP1-homologs in transgenic chrysanthemum under long-day conditions had no effect on plant development compared to non-transgenic
controls. Conversely, under short-day conditions, transgenic plants commenced bud initiation 2 wk earlier than non-transgenic
chrysanthemum plants. Subsequently, transgenic chrysanthemum flowers showed color earlier and resulted in full opening of
inflorescences 3 wk prior to non-transgenic control plants. These results open new possibilities for genetic improvement and
breeding of chrysanthemum cultivars. 相似文献
98.
99.
100.
Sibirtsev JT Shastina VV Menzorova NI Makarieva TN Rasskazov VA 《Marine biotechnology (New York, N.Y.)》2011,13(3):536-543
Previously, we have purified three distinct DNases from spermatozoa of sea urchin Strongylocentrotus intermedius and we suppose the role of Ca2+, Mg2+-dependent DNase (Ca, Mg-DNase) in apoptosis of spermatozoa. Two-headed sphingolipid rhizochalin (Rhz) induced characteristic
apoptotic nuclear chromatin changes, internucleosomal DNA cleavage, and activation of caspase-9, caspase-8, and caspase-3
in spermatozoa as was shown by fluorescence Hoechst 33342/PI/FDA analysis, DNA fragmentation assay, and fluorescence caspase
inhibitors FAM-LEHD-fmk, FAM-IETD-fmk, and FAM-DEVD-fmk, respectively. Inhibitor of caspase-3 z-DEVD-fmk subdued Rhz-induced
internucleosomal ladder formation, which confirmed the major role of caspase-3 in apoptotic DNA cleavage probably through
Ca, Mg-DNase activation. Participation of sea urchin Ca, Mg-DNase in apoptosis of spermatozoa was demonstrated by ions Zn2+ blocking of Rhz-induced DNA fragmentation due to direct inhibition of the Ca, Mg-DNase and internucleosomal cleavage of HeLa
S and Vero E6 cell nuclei chromatin by highly purified Ca, Mg-DNase. 相似文献