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731.
The present investigation deals with the determination of various serum enzymes known to be elevated during myocardial infarction
(MI) and estimation of selected metals like Cu, Cr, Co, Fe, Pb, and Mg by flame atomic absorption spectrophotometry. The data
obtained thereby were processed for the determination of correlation coefficient matrix among the cardiac enzymes and the
serum metals. The study evidenced the accumulation of Pb during MI and reduction in the level of Fe. A significant negative
correlation was observed between Cu and creatine kinase-MB. The data were also segregated into various groups to study the
influence of age and gender on the levels of selected parameters. In both the genders, the age of the patients was found to
be correlated significantly with various cardiac enzymes. In case of male patients, the most significant correlation was observed
between age and blood sugar at random. The other significant correlations among the male patients included Cr–CPK, Cr–creatine
kinase-MB, Fe–age, and others. In female patients, the pairs of studied parameters that exhibited significant correlations
included age–lactic dehydrogenase, creatine phosphokinase isoenzyme–aspartate aminotransferase, lactic dehydrogenase–creatine
phosphokinase isoenzymes, Pb-Fe, and Cu-Co in addition to others. 相似文献
732.
A new genus ofEncyrtidae is erected for one new species described from females and males taken in Pakistan. 相似文献
733.
734.
Alessandra Ricciardi Sasisekhar Bennuru Sameha Tariq Sukhbir Kaur Weiwei Wu Abdel G. Elkahloun Anush Arakelyan Jahangheer Shaik David W. Dorward Thomas B. Nutman Roshanak Tolouei Semnani 《PLoS neglected tropical diseases》2021,15(1)
We have previously shown that the microfilarial (mf) stage of Brugia malayi can inhibit the mammalian target of rapamycin (mTOR; a conserved serine/threonine kinase critical for immune regulation and cellular growth) in human dendritic cells (DC) and we have proposed that this mTOR inhibition is associated with the DC dysfunction seen in filarial infections. Extracellular vesicles (EVs) contain many proteins and nucleic acids including microRNAs (miRNAs) that might affect a variety of intracellular pathways. Thus, EVs secreted from mf may elucidate the mechanism by which the parasite is able to modulate the host immune response during infection. EVs, purified from mf of Brugia malayi and confirmed by size through nanoparticle tracking analysis, were assessed by miRNA microarrays (accession number ) and shown to be enriched (>2-fold, p-value<0.05, FDR = 0.05) for miR100, miR71, miR34, and miR7. The microarray analysis compared mf-derived EVs and mf supernatant. After confirming their presence in EVs using qPCR for these miRNA targets, web-based target predictions (using MIRPathv3, TarBAse and MicroT-CD) predicted that miR100 targeted mTOR and its downstream regulatory protein 4E-BP1. Our previous data with live parasites demonstrated that mf downregulate the phosphorylation of mTOR and its downstream effectors. Additionally, our proteomic analysis of the mf-derived EVs revealed the presence of proteins commonly found in these vesicles (data are available via ProteomeXchange with identifier PXD021844). We confirmed internalization of mf-derived EVs by human DCs and monocytes using confocal microscopy and flow cytometry, and further demonstrated through flow cytometry, that mf-derived EVs downregulate the phosphorylation of mTOR in human monocytes (THP-1 cells) to the same degree that rapamycin (a known mTOR inhibitor) does. Our data collectively suggest that mf release EVs that interact with host cells, such as DC, to modulate host responses. GSE157226相似文献
735.
736.
737.
Biotechnology Letters - Melatonin is a natural indolamine that regulates many physiological functions in plants. The most prominent role of melatonin in plants has been its ability to work as an... 相似文献
738.
There is much public interest in the use of fungal biological control agents as alternatives to chemical pesticides. However,
there are some concerns as to whether the metabolites produced by these fungi pose a risk to humans and the environment. Destruxins
are the main metabolites produced by the insect pathogenic fungus Metarhizium
anisopliae (Metsch.) Sorok. The production of these compounds in two different insect hosts and their subsequent fate in the soil were
assessed as a case study. Destruxin profiling revealed that the amount and type of destruxin produced was dependant upon the
fungal strain and insect host and that these compounds decomposed shortly after host death. Destruxin decomposition was presumably
due to the activity of hydrolytic enzymes in the cadavers and appeared to be independent of host or soil type and biota. Temperature
strongly influenced destruxin decomposition. Our studies are the first to show that the destruxins are essentially restricted
to the host and pathogen and are unlikely to contaminate the environment or enter the food chain. 相似文献
739.
Tariq Mesallati Conor T. Buckley Thomas Nagel Daniel J. Kelly 《Biomechanics and modeling in mechanobiology》2013,12(5):889-899
It remains unclear how specific mechanical signals generated by applied dynamic compression (DC) regulate chondrocyte biosynthetic activity. It has previously been suggested that DC-induced interstitial fluid flow positively impacts cartilage-specific matrix production. Modifying fluid flow within dynamically compressed hydrogels therefore represents a promising approach to controlling chondrocyte behavior, which could potentially be achieved by changing the construct architecture. The objective of this study was to first determine the influence of construct architecture on the mechanical environment within dynamically compressed agarose hydrogels using finite element (FE) modeling and to then investigate how chondrocytes would respond to this altered environment. To modify construct architecture, an array of channels was introduced into the hydrogels. Increased magnitudes of fluid flow were predicted in the periphery of dynamically compressed solid hydrogels and also around the channels in the dynamically compressed channeled hydrogels. DC was found to significantly increase sGAG synthesis in solid constructs, which could be attributed at least in part to an increase in DNA. DC was also found to preferentially increase collagen accumulation in regions of solid and channeled constructs where FE modeling predicted higher levels of fluid flow, suggesting that this stimulus is important for promoting collagen production by chondrocytes embedded in agarose gels. In conclusion, this study demonstrates how the architecture of cell-seeded scaffolds or hydrogels can be modified to alter the spatial levels of biophysical cues throughout the construct, leading to greater collagen accumulation throughout the engineered tissue rather than preferentially in the construct periphery. This system also provides a novel approach to investigate how chondrocytes respond to altered levels of biophysical stimulation. 相似文献
740.
Ali Ahmed Derar Derar R. Abdel-Elmoniem Essam M. Almundarij Tariq I. 《Biological trace element research》2020,193(1):162-165
Biological Trace Element Research - The objective of this study was to investigate the cadmium (Cd) profile in seminal plasma of male dromedary camel with impotentia generandi (post-coital... 相似文献