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41.
Through the application of a unique method for isolating plasma membranes, it was possible to specifically iodinate cytoplasm-exposed plasma membrane proteins in vegetative cells of the cellular slime mold Dictyostelium discoideum. The original procedure [Chaney, L. K., & Jacobson, B. S. (1983) J. Biol. Chem. 258, 10062] which involved coating cells with colloidal silica has been modified to yield a more pure preparation. The presence of the continuous and dense silica pellicle on the outside surface of the isolated plasma membrane permitted the specific labeling of cytoplasm-exposed membrane proteins. Lactoperoxidase-catalyzed iodination was employed to label cell-surface and cytoplasm-exposed membrane proteins. The isolated and radioiodinated membranes were then compared and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The cell-surface and cytoplasmic face labeling patterns were distinct. A total of 65 proteins were found to be accessible to at least one surface of the membrane. Sixteen intermolecular disulfide bond complexes were observed in the plasma membrane of Dictyostelium; most of these complexes involved glycoproteins and, hence, were exposed to the cell surface. 相似文献
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43.
Characterization of a novel acid phosphatase from embryonic axes of kidney bean exhibiting vanadate-dependent chloroperoxidase activity 总被引:1,自引:0,他引:1
Yoneyama T Shiozawa M Nakamura M Suzuki T Sagane Y Katoh Y Watanabe T Ohyama T 《The Journal of biological chemistry》2004,279(36):37477-37484
A novel colorless acid phosphatase (KeACP), which was distinct from the kidney bean purple acid phosphatase, was purified to apparent homogeneity and cloned from embryonic axes of kidney bean (Phaseolus vulgaris L. Ohfuku) during germination. When orthovanadate (VO(4)(-3)) is added to the apo form of the enzyme, KeACP uniquely exhibits the chloroperoxidase activity with loss of phosphatase activity. This is the first demonstration that KeACP is a vanadate-dependent chloroperoxidase in plants to be characterized and suggests that KeACP may play a role in modifying a wide variety of chlorinated compounds that are present in higher plants. The enzyme is a dimer that presents three forms made up of the combination of the dominant 56-kDa and the minor 45-kDa subunits, and both subunits contain carbohydrate. The full-length cDNA of the KeACP gene is 1641 nucleotides, and this sequence is predicted to encode a protein having 457 amino acid residues (52,865 Da), including a signal peptide. The complete nucleotide sequence of the genomic DNA (3228 bp) of KeACP consists of seven exons and six introns. Northern blot analysis demonstrated that the KeACP gene was expressed specifically in embryonic axes of the kidney bean, and its expression coincided with elongation of the embryonic axis during germination. 相似文献
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45.
E-Cell 2: multi-platform E-Cell simulation system 总被引:6,自引:0,他引:6
Takahashi K Ishikawa N Sadamoto Y Sasamoto H Ohta S Shiozawa A Miyoshi F Naito Y Nakayama Y Tomita M 《Bioinformatics (Oxford, England)》2003,19(13):1727-1729
A new version of the E-Cell simulation system,which runs on Windows as well as Linux, has been released as free software under the terms of the GNU General Public License. 相似文献
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47.
Gamma-glutamyl transpeptidase (gamma-GTP) is a membrane-bound enzyme which is known to play a crucial role in active transport of amino acids across membrane barriers. We prepared a monoclonal antibody recognizing specifically rat gamma-GTP and investigated localization of the enzyme in the rat brain by immunohistochemistry with this antibody. The antigen was localized on the ependyma, epithelia of the choroid plexus and microvessels. More precise localization of gamma-GTP was examined with immuno-electron microscopy. The antigen was recognized on the microvilli and cilia of the ependymal cells, microvilli of the choroid epithelial cells and luminal membranes of the vascular endothelial cells. 相似文献
48.
Takeshi Baba Nobuki Shiozawa Masao Hotchi Shinichi Ohno 《Virchows Archiv. B, Cell pathology including molecular pathology》1992,61(1):39-47
The three-dimensional ultrastructure of multinucleate giant cells in subcutaneous granulomas was compared with those of peritoneal
macrophages using a quick-freezing and deep-etching method. Subcutaneous granulomas were induced by implanting plastic coverslips
in the dorsal subcutaneous tissue of rats. The quick-freezing and deep-etching replicas were prepared from the cells attached
to the coverslips. Dense networks of actin filaments were distributed along all peripheral aspects (beneath the plasma membrane,
and on free and coverslip-attached surfaces) of the multinucleate giant cells. On the coverslip-attached surface, numerous
clathrin-coated pits and vesicles occurred between the actin filaments. In these cells, intermediate filaments, but not actin
filaments, were the predominant cytoskeletal components in perinuclear regions and were attached to the cell nucleus, mitochondria
and other vesicular cell organelles. A similar distribution of cytoskeletal components was observed in the mononuclear macrophages
of the granulomas and the peritoneal macrophages. These results show that the cytoskeletal organization varies in different
regions of the cytoplasm of multinucleate giant cells, while the characteristic cytoskeletal arrangement, resembling that
of mononuclear macrophages, is maintained. 相似文献
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Localization of lipocaline-type prostaglandin D synthase in rat brain: immunoelectron microscopic study 总被引:1,自引:1,他引:0
Nagasaka T Hiraide M Sugimoto T Shindo K Shiozawa Z Yokota S 《Histochemistry and cell biology》2004,121(6):483-491
The distribution of lipocaline-type prostaglandin D synthase (L-PGDS) in rat brain was investigated by immunoelectron microscopy using a protein A-gold technique. In perivascular cells adjacent to the basement membrane of arterioles in the pia-arachnoid and of blood vessels in the subpial cortex, gold labeling was confined to the lumen of the dilated rough endoplasmic reticulum, and not found in the few lysosomes present in the cytoplasm. The results suggest that the perivascular cells secrete L-PGDS and seem not to degrade lipophilic molecules carried by L-PGDS. Moreover, gold particles representing the antigenic sites of L-PGDS were found in the Golgi apparatus, rough endoplasmic reticulum, vesicles, and nuclear envelope of arachnoid trabecular cells, arachnoid barrier cells, and arachnoid pia mater cells. The labeling was less detectable in the same organelles of choroid plexus epithelial cells, compared with leptomeningeal cells. In meningeal macrophages and parenchymal microglia, L-PGDS was detected in lysosomes, multivesicular bodies, and endocytic vesicles. The production of L-PGDS in perivascular cells is important to the various functions of this enzyme in brain parenchyma. 相似文献