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41.
Fábio Takenori Higa Lucila Okuyama Fukasawa Maria Gisele Gon?alves Maristela Marques Salgado Ana Paula Silva de Lemos Lee H Harrison Priscilla Lima de Oliveira Carla Naufal da Silva Claudio Tavares Sacchi 《Memórias do Instituto Oswaldo Cruz》2013,108(2):246-247
We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution. 相似文献
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Takenori Tomohiro Hirotsugu Inoguchi Souta Masuda Yasumaru Hatanaka 《Bioorganic & medicinal chemistry letters》2013,23(20):5605-5608
A specific illumination approach has been developed for identification of adenosine triphosphate (ATP)-binding proteins. This strategy utilizes a tandem photoactivatable unit that consists of a diazirine group as a carbene precursor and an o-hydroxycinnamate moiety as a coumarin precursor. The photolysis of diazirine induces a specific cross-link on target proteins and is followed by photoactivation of coumarin generation with a concomitant release of the pre-installed affinity ligand. The ATP, installed with this cross-linker at the γ-position, successfully transferred a coumarin onto ATP-binding proteins using only UV-irradiation. 相似文献
43.
Akira Hayashi Kyosuke Kinoshita Yoshihiko Kotani 《Bioscience, biotechnology, and biochemistry》2013,77(8):1705-1709
The phase change for an amylose–butanol complex solution in 10% of dimethylsulfoxide was investigated as a function of temperature. The phase change was determined with measurements of the turbidity, fluorescent depolarization, and viscosity. The phase diagram obtained was qualitatively similar to that for an amylose solution. From the result, the change in solution phase for the amylose–butanol complex is suggested to be similar to that for amylose, i.e., when the solution cools from a higher temperature, amylose molecules in the complex solution change the conformation from a random coil to an interrupted helix, and then separate into two phases. Coacervate particles resulting from the phase separation coalesce with each other to yield precipitates.An adsorption of uranine on amylose was studied to ascertain its relationship with the fluorescent depolarization method used for detecting phase changes in solution. The result showed that uranine was adsorbed on amylose chains but not on the amylose–butanol complex. 相似文献
44.
Masakazu Inoue Tatsuko Egami Kanae Yokogawa Hirotada Kotani Toshio Morioka 《Bioscience, biotechnology, and biochemistry》2013,77(7):1391-1400
Cariogenic streptococci produce tenacious water-insoluble polysaccharides from sucrose and these form the structural intercellular matrix of dental plaque. Two Streptomyces species were isolated from soils on agar medium containing the water-insoluble polyglucan as a sole carbon source. They were identified as Streptomyces werraensis (strain F1) and Streptomyces chartreusis (strain F2). These strains produced extracellular enzymes which strongly solubilized the polyglucans from various strains of cariogenic streptococci. Strain F2 produced polyglucanases under rather stationary cultural condition, while F1 required vigorous aeration. The polyglucanases may provide a useful measure for the prevention and control of dental plaque formation, 相似文献
45.
Seiichi Nonomura Ryotaro Kotani Rintaro Urakabe Shoji Shima Heiichi Sakai 《Bioscience, biotechnology, and biochemistry》2013,77(9):1755-1756
A series of partially and heterogeneously N-acylated chitosans was prepared and isolated in 50 ~ 100% yields. The structure of N-acyl groups influenced the gelation. The minimum requirement for the gelation was defined as ca. 0.4 N-lauroyl (C12), ca. 0.6 N-fatty acyl (C3–C10) or ca. 0.7 N-benzoyl groups per hexosaminide residue. However, the gelation did not occur with N-high fatty acyl (C14-C18) groups.1) 相似文献
46.
Journal of Ichthyology - The Serranidae are well known for protogynous sex change. The red-belted anthias Pseudanthias rubrizonatus inhabits Kagoshima Bay. We aimed to estimate the body size and... 相似文献
47.
Stability and bifurcation analysis of a ratio-dependent community dynamics model on Batesian mimicry
Journal of Mathematical Biology - Batesian mimicry is the similarity of coloration and patterns in an unpalatable species (the “model-species”) and a palatable species (the... 相似文献
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Molecular characterization of Bombyx mori cytoplasmic polyhedrosis virus genome segment 4 总被引:6,自引:0,他引:6 下载免费PDF全文
Ikeda K Nagaoka S Winkler S Kotani K Yagi H Nakanishi K Miyajima S Kobayashi J Mori H 《Journal of virology》2001,75(2):988-995
The complete nucleotide sequence of the genome segment 4 (S4) of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) was determined. The 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kDa. Western blot analysis showed that S4 encodes BmCPV protein VP3, which is one of the outer components of the BmCPV virion. Sequence analysis of the deduced amino acid sequence of BmCPV VP3 revealed possible sequence homology with proteins from rice ragged stunt virus (RRSV) S2, Nilaparvata lugens reovirus S4, and Fiji disease fijivirus S4. This may suggest that plant reoviruses originated from insect viruses and that RRSV emerged more recently than other plant reoviruses. A chimeric protein consisting of BmCPV VP3 and green fluorescent protein (GFP) was constructed and expressed with BmCPV polyhedrin using a baculovirus expression vector. The VP3-GFP chimera was incorporated into BmCPV polyhedra and released under alkaline conditions. The results indicate that specific interactions occur between BmCPV polyhedrin and VP3 which might facilitate BmCPV virion occlusion into the polyhedra. 相似文献