全文获取类型
收费全文 | 148篇 |
免费 | 4篇 |
专业分类
152篇 |
出版年
2022年 | 1篇 |
2019年 | 2篇 |
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 1篇 |
2014年 | 4篇 |
2013年 | 23篇 |
2012年 | 6篇 |
2011年 | 10篇 |
2010年 | 4篇 |
2009年 | 1篇 |
2008年 | 8篇 |
2007年 | 7篇 |
2006年 | 6篇 |
2005年 | 11篇 |
2004年 | 11篇 |
2003年 | 10篇 |
2002年 | 4篇 |
1999年 | 2篇 |
1998年 | 7篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 6篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1976年 | 2篇 |
排序方式: 共有152条查询结果,搜索用时 15 毫秒
101.
Nobutaka Someya Masami Nakajima Ken Watanabe Tadaaki Hibi Katsumi Akutsu 《Biocontrol Science and Technology》2005,15(1):105-109
Serratia marcescens strain B2 inhibited mycelial growth of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA. Rice plants were treated with bacterial suspension and then challenge inoculated with the pathogen. Application of S. marcescens effectively reduced the incidence of sheath blight. S. marcescens survived in soil under glasshouse conditions at ca. 108 colony forming units g-1 of soil for 4 weeks after application. These results suggest that S. marcescens has potential as an effective and persistent biological control agent for rice sheath blight. 相似文献
102.
Inoue Y Ogasawara M Moroi T Satake M Azumi K Moritomo T Nakanishi T 《Immunogenetics》2005,57(7):520-534
To illuminate the origins of NADPH oxidase (Nox), we identified cDNA clones encoding Nox2, Nox4, p22 phagocyte oxidase (phox),
p47phox, and p67phox in a chordate phylogenetically distant to the vertebrates, the sea squirt Ciona intestinalis. We also examined the spatiotemporal expression of these genes in embryos and juveniles. The sequences of the Nox2, Nox4,
p22phox, p47phox, and p67phox cDNAs contained open reading frames encoding 581, 811, 175, 461, and 515 amino acids, respectively.
The level of identities between the deduced Nox2, Nox4, p22phox, p47phox, and p67phox amino acid sequences and their corresponding
human components were 54.0, 31.0, 44.4, 36.0, and 26.2%, respectively. Despite these low identities, the functional domains
of the C. intestinalis and human NADPH oxidase and Nox4 are highly conserved. The genomic organizations of the components of the NADPH oxidase gene
except for p67phox (a single exon gene) and the Nox4 gene in C. intestinalis are highly similar to those of the corresponding human NADPH oxidase genes. Further, the analyzed part of the C. intestinalis genome and EST database do not seem to present p40phox and Nox5. The Nox2, p22phox, p47phox, and p67phox genes were specifically
expressed in the blood cells of juveniles. The Nox4 gene was expressed in blood cells and endostyle of juveniles. These results
suggest that C. intestinalis NADPH oxidase components possess potential functional activities similar to those of human, but the manner in which cytosolic
phox proteins in C. intestinalis interact is different from that in human. 相似文献
103.
Obi Ichiro; Ichikawa Yoshiaki; Nonaka Ryuichi; Kakutani Tadaaki; Senda Mitsugi 《Plant & cell physiology》1989,30(5):759-764
Electrophoretic mobilities of barley mesophyll cell protoplastsmodified by chemical and enzymatic treatments were measuredin media at various pH values to elucidate the contributionof phosphate, carboxylate and amino groups to the surface chargedensity. Existence of these charged groups was confirmed byresults of treatment of protoplasts with glutaraldehyde (foramino groups), acid phosphatase (for phosphate groups) and l-ethyl-3-(3-dimethylaminopropyl)carbodi-imidetogether with glycine methyl ester (for carboxylate groups).The relative amounts of these groups were estimated from thecurves of surface charge density () vs. surface pH (pHs) ofthe treated protoplasts, in terms of simplified acid-base dissociationcurves. The estimated ratio of the amounts of phosphate, carboxylateand amino groups was approximate 0.5 :0.5 : 0.7 for the native(unmodified) barley mesophyll cell protoplasts, when the totalnegative charge on the native protoplasts was assumed to be1. (Received January 9, 1989; Accepted April 28, 1989) 相似文献
104.
105.
A Critical Role for Cyclin C in Promotion of the Hematopoietic Cell Cycle by Cooperation with c-Myc 总被引:5,自引:1,他引:4 下载免费PDF全文
106.
Sequence variation, differential expression and chromosomal location of rice chitinase genes 总被引:1,自引:0,他引:1
Yoko Nishizawa Naoki Kishimoto Akira Saito Tadaaki Hibi 《Molecular & general genetics : MGG》1993,241(1-2):1-10
Rice chitinases are encoded by a small multigene family. To clarify the overall organization of rice chitinase genes, we have isolated and characterized the genes Cht-1, Cht-2 and Cht-3. Although all the three genes encode class I chitinase, the nucleotide sequences of the coding regions of Cht-1 and Cht-3 are very similar (90%), while that of Cht-2 is clearly more divergent (78%). Only Cht-2 has a 130 by intron and encodes a C-terminal peptide sequence similar to that known to function as a vacuolar targeting signal. In 5 flanking regions of Cht-1 and Cht-3, but not of Cht-2, conserved sequences (GGCCGGCYGCCCYAG) were found. Related sequences were found also in the 5 flanking regions of another chitinase gene and a -glucanase gene which has also been reported to be stress-induced in rice. RNA blot hybridization analysis demonstrated that the stress-induced expression patterns of the Cht-1 and Cht-3 genes are similar, but quite different from that of Cht-2. However, all three genes are active in unstressed roots. By restriction fragment length polymorphism (RFLP) linkage analysis, Cht-1 and Cht-3 were mapped onto chromosome 6 and shown to be closely linked (0.8 cM). Cht-2 was mapped onto chromosome 5. All these features suggest that the expression patterns of rice class I chitinase genes may be correlated with their levels of sequence divergence and their chromosomal location. 相似文献
107.
Summary Marigold (Tagetes patula L.) hairy roots induced by infection with Agrobacterium rhizogenes produced -terthienyl when grown in darkness, and an n-hexane extract of the roots showed nematocidal activity. Depending on the hairy root line used, the level of -terthienyl varied from 15 to 1268 g per g dry weight, a level that corresponded to 0.15 to 12.7-fold that in intact roots. Analysis by HPLC indicated that the nematocidal activity was due predominantly to -terthienyl. However, it is suggested that nematocidal compounds other than -terthienyl are present in hairy roots cultured in the dark for long periods or in the light. 相似文献
108.
Changes in muscle sympathetic nerve activity and calf blood flow during static handgrip exercise 总被引:2,自引:0,他引:2
Mitsuru Saito Tadaaki Mano Satoshi Iwase 《European journal of applied physiology and occupational physiology》1990,60(4):277-281
To test the function of sympathetic vasco-constrictor nerves on blood flow in resting limbs during static muscle contraction, muscle sympathetic nerve activity (MSNA) to the leg muscle was recorded from the tibial nerve microneurographically before, during and after 2 min of static handgrip (SHG). Simultaneously, calf blood flow (CBF) was measured by strain gauge plethysmography. An increase in MSNA, a decrease in CBF and an increase in calf vascular resistance (CVR) in the same resting limb occurred concomitantly during SHG. However, the increase in CVR was blunted in the second minute of handgrip when MSNA was still increasing. The results indicated that the decrease of CBF during SHG reflects the increase in MSNA, while the dissociation between MSNA and CVR at the later period of SHG may be related to metabolic change produced by the vasoconstriction. 相似文献
109.
Tomoaki Koshizawa Toshiharu Morimoto Gen Watanabe Tomoaki Fukuda Nao Yamasaki Sumihiko Hagita Yoshikazu Sawada Ayumu Okuda Kimiyuki Shibuya Tadaaki Ohgiya 《Bioorganic & medicinal chemistry letters》2018,28(19):3236-3241
Herein, we describe the discovery, synthesis, and evaluation of a novel series of spiro[chromane-2,4′-piperidine] derivatives as G-protein-coupled receptor 119 agonists. Their initial design exploited the conformational restriction in the linker-to-tail moiety, which was a key concept in this study, to give lead compound 11 (EC50?=?369?nM, Emax?=?82%). An extensive structure–activity relationship study resulted in the identification of the optimized drug candidate (R)-29 (EC50?=?54?nM, Emax?=?181%). The defining structural features of the series were a terminal benzyl-type bulky substituent and a methylene linker between the sulfonyl and phenyl groups, both of which were in the head moiety as well as the spiro-type scaffold in the linker-to-tail moiety. An in vivo oral glucose-tolerance test using C57BL/6N mice showed that (R)-29 reduced glucose excursion at a dose of 3?mg/kg in a dose-dependent manner. 相似文献
110.
Removal of shoot tops from mulberry plants in autumn hastenedthe accumulation of starch in the stems even though photosynthesisin the remaining uppermost leaves was supressed. The proportionof the amount of 14CO2 assimilate taken into the veins and thecontents of foliar carbohydrates and protein were not affectedby pruning. (Received December 22, 1983; Accepted March 21, 1984) 相似文献