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排序方式: 共有260条查询结果,搜索用时 140 毫秒
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Effects of seven potential probiotic strains on specific immune responses in healthy adults: a double-blind, randomized, controlled trial 总被引:1,自引:0,他引:1
Paineau D Carcano D Leyer G Darquy S Alyanakian MA Simoneau G Bergmann JF Brassart D Bornet F Ouwehand AC 《FEMS immunology and medical microbiology》2008,53(1):107-113
This pilot study investigated the immunomodulatory properties of seven probiotic strains. Eighty-three healthy volunteers aged 18-62 years consumed 2 x 10(10) CFU of bacteria or a placebo (maltodextrin) over 3 weeks (D0-D21). Subjects received an oral cholera vaccine at D7 and at D14; blood and saliva samples were collected at D0, D21 and D28. Serum samples were analyzed for specific IgA, IgG and IgM, and saliva samples were analyzed for specific IgA only, by ELISA. Statistical analyses were based on Wilcoxon's signed-rank test (intragroup analyses) and exact median t-test (intergroup analyses). Salivary analysis showed no difference in specific IgA concentrations between groups. Serum analysis indicated an effect of some of the tested strains on specific humoral responses. Between D0 and D21, IgG increased in two probiotic groups, for example, Bifidobacterium lactis Bl-04 and Lactobacillus acidophilus La-14, compared with controls (P=0.01). Trends toward significant changes in immunoglobulin serum concentrations compared with controls (P<0.1) were found for six out of the seven probiotic strains. In conclusion, some strains of probiotics demonstrated a faster immune response measured with serum immunoglobulin indicators, especially IgG, although overall vaccination was not influenced. Specific strains of probiotics may thus act as adjuvants to the humoral immune response following oral vaccination. 相似文献
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Fishing for lectins from diverse sequence libraries by yeast surface display - an exploratory study 总被引:1,自引:0,他引:1
The establishment of a robust technology platform for the expression cloning of carbohydrate-binding proteins remains a key challenge in glycomics. Here we explore the utility of using yeast surface display (YSD) technology in the interaction-based lectin cloning from complete cDNA libraries. This should pave the way for more detailed studies of protein-carbohydrate interactions. To evaluate the performance of this system, lectins representing three different subfamilies (galectins, siglecs, and C-type lectins) were successfully displayed on the surface of Saccharomyces cerevisiae and Pichia pastoris as a-agglutinin and/or alpha-agglutinin fusions. The predicted carbohydrate-binding activity could be detected for three out of five lectins tested (galectin-1, galectin-3, and siaoadhesin). For galectin-4 and E-selectin, no specific carbohydrate-binding activity could be detected. We also demonstrate that proteins with carbohydrate affinity can be specifically isolated from complex metazoan cDNA libraries through multiple rounds of FACS sorting, employing multivalent, fluorescent-labeled polyacrylamide-based glycoconjugates. 相似文献
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Roland Abi Nahed Guillaume Martinez Jessica Escoffier Sandra Yassine Thomas Karaouzène Jean-Pascal Hograindleur John Turk George Kokotos Pierre F. Ray Serge Bottari Gérard Lambeau Sylviane Hennebicq Christophe Arnoult 《The Journal of biological chemistry》2016,291(6):3076-3089
Phospholipase A2 (PLA2) activity has been shown to be involved in the sperm acrosome reaction (AR), but the molecular identity of PLA2 isoforms has remained elusive. Here, we have tested the role of two intracellular (iPLA2β and cytosolic PLA2α) and one secreted (group X) PLA2s in spontaneous and progesterone (P4)-induced AR by using a set of specific inhibitors and knock-out mice. iPLA2β is critical for spontaneous AR, whereas both iPLA2β and group X secreted PLA2 are involved in P4-induced AR. Cytosolic PLA2α is dispensable in both types of AR. P4-induced AR spreads over 30 min in the mouse, and kinetic analyses suggest the presence of different sperm subpopulations, using distinct PLA2 pathways to achieve AR. At low P4 concentration (2 μm), sperm undergoing early AR (0–5 min post-P4) rely on iPLA2β, whereas sperm undergoing late AR (20–30 min post-P4) rely on group X secreted PLA2. Moreover, the role of PLA2s in AR depends on P4 concentration, with the PLA2s being key actors at low physiological P4 concentrations (≤2 μm) but not at higher P4 concentrations (∼10 μm). 相似文献
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J. Marc Fremy Lilian Puech Sophie Krys Sylviane Dragacci 《Journal of applied phycology》1999,11(4):377-384
Okadaic acid and dinophysistoxins are produced by some marine unicellular algae from the plankton and also benthic microalgae
and may accumulate in shellfish. These phycotoxins are involved in a gastrointestinal syndrome called diarrhetic shellfish
poisoning (DSP), which occurs in humans after consumption of bivalve molluscs. Thousands cases of human poisonings in Europe
were caused by consumption of toxic shellfish during the past decade. The rapid detection and the reliable determination of
the main phycotoxins implicated in DSP are a major concern for governmental institutions in charge of the sanitary control
of seafood safety. Analytical procedures for the detection and determination of DSP toxins can be classified as: bioassays,
biochemical methods including immunoassays, or physicochemical methods. Although a large number of methods have been developed,
none have been officially validated. A complete panel of tools for DSP toxin analysis should include screening, investigation,
and confirmation methods. This paper presents a compilation of recent developments and optimisations of these methods.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Carine Chavey Gwendal Lazennec Sylviane Lagarrigue Cyrielle Clapé Irena Iankova Jacques Teyssier Jean-Sébastien Annicotte Julien Schmidt Chikage Mataki Hiroyasu Yamamoto Rosario Sanches Anna Guma Vladimir Stich Michaela Vitkova Bénédicte Jardin-Watelet Eric Renard Robert Strieter Antoinette Tuthill Gôkhan S. Hotamisligil Antonio Vidal-Puig Lluis Fajas 《Cell metabolism》2009,9(4):339-349