全文获取类型
收费全文 | 912篇 |
免费 | 43篇 |
国内免费 | 1篇 |
出版年
2023年 | 3篇 |
2022年 | 4篇 |
2021年 | 21篇 |
2020年 | 13篇 |
2019年 | 10篇 |
2018年 | 24篇 |
2017年 | 20篇 |
2016年 | 27篇 |
2015年 | 27篇 |
2014年 | 41篇 |
2013年 | 60篇 |
2012年 | 81篇 |
2011年 | 67篇 |
2010年 | 44篇 |
2009年 | 36篇 |
2008年 | 47篇 |
2007年 | 57篇 |
2006年 | 47篇 |
2005年 | 44篇 |
2004年 | 43篇 |
2003年 | 41篇 |
2002年 | 28篇 |
2001年 | 7篇 |
2000年 | 9篇 |
1999年 | 10篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 10篇 |
1995年 | 7篇 |
1994年 | 8篇 |
1993年 | 6篇 |
1992年 | 10篇 |
1991年 | 6篇 |
1990年 | 8篇 |
1989年 | 4篇 |
1988年 | 5篇 |
1987年 | 9篇 |
1986年 | 9篇 |
1985年 | 5篇 |
1984年 | 3篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1977年 | 5篇 |
1976年 | 6篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1966年 | 2篇 |
排序方式: 共有956条查询结果,搜索用时 390 毫秒
951.
952.
Decolorization of three triphenylmethane dyes by three bird's nest fungi—Cyathus bulleri, C. stercoreus, and C. striatus—was studied. Cyathus bulleri was found to be the most efficient in decolorization as demonstrated by the disappearance of the dyes from cultures, monitored by decreases in absorbance. Growth of the Cyathus spp. was not affected by the presence of dyes in the cultures. Decolorization of dyes was also observed with extracellular culture filtrates, indicating exocellular activity. Laccase activity, tested in replicate cultures, was found to be maximum during the decolorization period. Nitrogen in the medium had no effect on decolorization. 相似文献
953.
Triacylglycerol ester hydrolase was isolated from bat adipose tissue and characterized. The partially purified enzyme had
pH optimum of 8.6 and a Km value of 0.6 mM. The enzyme was denaturated upon freezing and thawing, which was prevented by 25% glycerol. The enzyme was
activated by EDTA and NaCl, while it was inhibited by serum and bovine serum albumin. Heparin, sodium fluoride and diisopropyl
fluorophosphate had no effect on triacylglycerol ester hydrolase activity. It hydrolyzed triglycerides partially. Triacylglycerol
ester hydrolase lost its activity during delipidation but it was reactivated by endogenous lipids and phospholipids, viz.
phosphatidyl ethanolamine, phosphatidyl choline and sphingomyelin. The enzyme shows kinetic properties altogether different
from lipoprotein lipase and hormone sensitive lipase 相似文献
954.
A polyvalent radiovaccine of Salmonella was shown to induce protective immunity in mice. The results revealed that the immunized mice progressively eliminated the challenged organisms and no Salmonella could be isolated after a period of 21 days. In contrast, Salmonella grew in the control mice and reached levels of 10(8) to 10(9) cfu/spleen resulting in the death of animals. Sera from both control and the immune mice were found to lack bactericidal activity. It was further observed that the vaccine induced delayed type of hypersensitivity and that antibody production as measured by bacterial agglutination and passive hemagglutination were low in response to the vaccine. However, the phagocytic activity of the reticuloendothelial system was considerably enhanced by the immunization. The results of experiments with immunosuppressed mice suggested the role of thymus derived (T) lymphocytes in the protection. 相似文献
955.
956.
Curtis S. Fullmer Subhash Chandra Christina A. Smith George H. Morrison Robert H. Wasserman 《Histochemistry and cell biology》1996,106(2):215-222
A combination of ion microscopic and conventional radionuclide techniques was employed to investigate the temporal-spatial
dynamics of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]-stimulated intestinal calcium (Ca) absorption. At varying times following the administration of a single intravenous dose
of 1,25(OH)2D3, to vitamin D-deficient chicks, transepithelial transport and tissue retention of Ca were quantitated in vivo, using the
ligated duodenal loop technique and47Ca as the tracer. The localization of Ca in the intestinal tissue during absorption was monitored by ion microscopy, using
the stable Ca isotope,44Ca, as the absorbed species. There was little transepithelial absorption of Ca in the vitamin D-deficient animals despite
a substantial tissue accumulation of luminally derived Ca, the latter localizing predominantly in the brush border region
of the enterocyte, as shown by the44Ca-ion microscopic images. The early (30 min-1 h) response to 1,25(OH)2D3 was an increased tissue uptake of luminal47Ca, which also primarily associated with the brush border region, again as shown by ion microscopy. At 2–4 h after the 1,25(OH)2)D3 dose, there was a progressive redistribution of Ca from the brush border region throughout the cytoplasm and into the lamina
propria. At 8–16 h,47Ca absorption was maximal and44Ca was sparsely distributed in the intestinal tissue.47Ca absorption gradually declined and reached pre-dose levels by 72 h. At this time, tissue44Ca was again largely limited to the brush border region. These results provide support for the multiple actions of 1,25(OH)2D3 on the intestinal Ca absorption 相似文献