全文获取类型
收费全文 | 1629篇 |
免费 | 234篇 |
出版年
2021年 | 24篇 |
2020年 | 15篇 |
2019年 | 20篇 |
2018年 | 21篇 |
2017年 | 21篇 |
2016年 | 42篇 |
2015年 | 43篇 |
2014年 | 43篇 |
2013年 | 77篇 |
2012年 | 69篇 |
2011年 | 68篇 |
2010年 | 52篇 |
2009年 | 35篇 |
2008年 | 70篇 |
2007年 | 81篇 |
2006年 | 72篇 |
2005年 | 56篇 |
2004年 | 52篇 |
2003年 | 46篇 |
2002年 | 47篇 |
2001年 | 54篇 |
2000年 | 38篇 |
1999年 | 40篇 |
1998年 | 25篇 |
1997年 | 17篇 |
1996年 | 24篇 |
1995年 | 24篇 |
1994年 | 19篇 |
1993年 | 17篇 |
1992年 | 43篇 |
1991年 | 35篇 |
1990年 | 33篇 |
1989年 | 42篇 |
1988年 | 32篇 |
1987年 | 40篇 |
1986年 | 29篇 |
1985年 | 38篇 |
1984年 | 24篇 |
1983年 | 29篇 |
1982年 | 23篇 |
1980年 | 12篇 |
1979年 | 17篇 |
1978年 | 12篇 |
1977年 | 22篇 |
1976年 | 11篇 |
1975年 | 17篇 |
1973年 | 11篇 |
1971年 | 14篇 |
1969年 | 10篇 |
1967年 | 10篇 |
排序方式: 共有1863条查询结果,搜索用时 812 毫秒
101.
Morphogenetic movements and multicellular development in the fruiting Myxobacterium, Stigmatella aurantiaca. 总被引:5,自引:0,他引:5
Stigmatella aurantiaca, strain DW-4, is a bacterium that grows as single cells in liquid culture but will synchronously aggregate and construct multicellular fruiting bodies when starved on an agar surface. The fruiting body consists of a stalk and several sporangia housing differentiated myxospores. Fruiting body development is stimulated by exposure of the aggregating cells to incandescent light. 相似文献
102.
Carol M. Warner Ruth M. Graves Carla M. Tollefson Mary Jo F. Schmerr Thomas J. Stephens Carmen F. Merryman Paul H. Maurer 《Immunogenetics》1976,3(1):337-348
The immune response of allophenic mice of type C57BL/6(A × SJL) F1 to GL administered in complete Freund's adjuvant was tested. Control mice of the three strains C57BL/6, A, and SJL are all nonresponders to this antigen. However, the F1 generations of C57BL/6 × A, C57BL/6 × SJL, and A × SJL were all responders to the antigen, so that the complementarity of at least two genes is confirmed. The allophenic mice showed no further complementation beyond the F1 generation, a result which may argue against the possibility that more than two genes control the response to GL in these mouse strains. Characterization of the allophenic mice over several months showed that they exhibit chimeric drift, both in their coat color and in peripheral white blood cell population. There is no apparent correlation of coat color to the lymphocyte composition of the mice at any one time. The mice are true chimeras, since killing of the two populations of white blood cells with two different anti-H-2 sera produced a 100 percent killing. The immune response of individual allophenic mice to GL showed a good correlation to the number of A × SJL lympho-cytes in the animal.Abbreviations used in this paper are GL
an amino acid polymer of 57 %l-glutamic acid, 38%l-lysine, and 5%l-phenylalanine
- GLT15
an amino acid polymer ofl-glutamic acid,l-lysine, and 15 %l-tyrosine
- (T,G)-A-L
an amino acid polymer having a polylysine backbone with side chains of polyd-l-alanine, terminating in short sequences of tyrosine and glutamic acid
- GAT10
an amino acid polymer of 60%l-glutamic acid, 30%l-alanine, and 10%l-tyrosine
- GLA5
an amino acid polymer of 57%l-glutamic acid, 38%l-lysine, and 5%l-alanine
- DNP
2,4 dinitrophenyl
- BGG
bovine gamma globulin
- FCS
fetal calf serum
- PWBC
peripheral white blood cell
- SWBC
spleen white blood cell
- T cell
thymus-derived lymphocyte
- B cell
bone marrow-derived lymphocyte 相似文献
103.
A potential enzymic mechanism for the degradation of glycosaminogly cans was characterised using enzymes found in rheumatoid synovial fluid from the knee joint. This mechanism involves a true hyluronidase together with the concerted action of beta-glucuronidase and beta-N-acetylhexosaminidase. The contribution of the exopolysaccharidases to hyaluronate degradation was demonstrated by the use of specific inhibitors, while the distinct identity of a true hyaluronidase was shown by ammonium sulphate and agarose gel column fractionations. Only the hyluronidase fraction was capable of degrading high molecular weight hyaluronate. The exopolysaccharidase activities were shown to be markedly elevated in rheumatoid as compared to osteoarthritic synovial fluid and also normal serum. On the other hand, hyluronidase was similarly active in rheumatoid and osteoarthritic synovial fluids; both these levels were lower than that of normal human serum. Hyaluronidase in synovial fluid may thus be derived by diffusion from serum, since it is of relatively low molecular weight (60 000). The pH requirements of this enzyme system and the strong inhibition of hyaluronidase by synovial fluid make it unlikely that the mechanism operates extracellularly. It is proposed that as a lysosomal mechanism, however, it is an important contributing factor in the chronic erosion process characteristic of rheumatoid arthritis. 相似文献
104.
Demonstration of a tumor-associated surface antigen in Marek's disease. 总被引:13,自引:0,他引:13
R L Witter E A Stephens J M Sharma K Nazerian 《Journal of immunology (Baltimore, Md. : 1950)》1975,115(1):177-183
Surface antigenic markers were detected on three classes of Marek's disease (MD) tumor cells, i.e., MD lymphoma cells, cultured cells of the MSB-1 lymphoblastoid cell line, and JMV lymphoblastic leukemia cells, by indirect membrane immunofluorescent staining with serum from chickens immunized with JMV cells or from rabbits immunized with MSB-1 cells. This surface antigen was not detected on normal chicken lymphocytes, RPL-16 tumor cells (tranedormed by an avian RNA virus, or MD virus-infected fibroblasts that were positive for viral membrane antigen (MA). Furthermore, the surface antigen appeared unrelated to embryonic or histocompatibility antigens. This antigen is provisionally designated as a Marek's disease tumor-associated surface antigen (MATSA). The MATSA's on JMV, MSB-1 and MD lymphoma cells were related but not identical as demonstrated by antiserum titration, absorption and blocking tests with homologous and heterologous systems. 相似文献
105.
P A Lorkin A D Stephens M E Beard P F Wrigley L Adams H Lehmann 《BMJ (Clinical research ed.)》1975,4(5990):200-202
A new haemoglobin with increased oxygen affinity, beta82 (EF6) lysine leads to threonine (Hb Rahere), was found during the investigation of a patient who was found to have a raised haemoglobin concentration after a routine blood count. The substitution affects one of the 2, 3-diphosphoglycerate binding sites, resulting in an increased affinity for oxygen, but both the haem-haem interaction and the alkaline Bohr effect are normal in the haemolysate. This variant had the same mobility as haemoglobin A on electrophoresis at alkaline pH but was detected by measuring the whole blood oxygen affinity; it could be separated from haemoglobin A, however, by electrophoresis in agar at acid pH. The raised haemoglobin concentration was mainly due to a reduction in plasma volume (a relative polycythaemia) and was associated with a persistently raised white blood count. This case emphasises the need to measure the oxygen affinity of haemoglobin in all patients with absolute or relative polycythaemia when some obvious cause is not evident. 相似文献
106.
107.
108.
Adjuvant disease-prevention with complete Freund's adjuvant and with mineral oil alone 总被引:1,自引:0,他引:1
109.
Ouimette Andrew P. Ollinger Scott V. Lepine Lucie C. Stephens Ryan B. Rowe Rebecca J. Vadeboncoeur Matthew A. Tumber-Davila Shersingh J. Hobbie Erik A. 《Ecosystems》2020,23(4):715-729
Ecosystems - Carbon (C) fluxes among different components of plant growth are important to forest ecosystem C cycling and are strongly influenced by species composition and resource availability.... 相似文献
110.