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71.
Souichi Nukuzuma Chiyoko Nukuzuma Masanori Kameoka Shigeki Sugiura Kazuo Nakamichi Takafumi Tasaki Koushi Hidaka Tsutomu Takegami 《Microbiology and immunology》2018,62(8):524-530
72.
Kousaku Matsubara Masaru Kubota Katsuji Kuwakado Haruyo Hirota Yoshihiro Wakazono Akiro Okuda Rikimaru Bessho Ying Wei Lin Souichi Adachi Yuichi Akiyama 《Experimental cell research》1994,213(2)
We recently reported that treatment with calcium ionophore, A23187, induces apoptosis in human myclogenous leukemia cells but causes necrotic cell death in T-lymphoblastic leukemia cells. To better understand the underlying mechanisms of such different modes of cell death, we established hybridomas between HL-60 promyelocytic and CEM T-lymphoblastic leukemia cells. The resulting hybridomas were divided into three groups in terms of their susceptibility to apoptosis following exposure to A23187: (1) hybridomas highly sensitive to apoptosis, (2) hybridomas with intermediate sensitivity to apoptosis which occurs later and to a lesser extent, and (3) hybridomas resistant to apoptosis. However, growth inhibition after 72 h of incubation and an initial rise in intracellular free calcium concentrations induced by A23187 were similar in the three groups. Expression of Ca2+-independent/Mg2+-dependent endonuclease, which had an optimal pH of 7.5-8.5 and was inhibited by Zn2+, was correlated with the susceptibility of the hybridomas to A23187-induced apoptosis. Thus, this endonuclease may play, at least in part, an important role in the induction of apoptosis in leukemia cell lines. Analysis of hybridomas between apoptosis-sensitive and apoptosis-resistant cells is useful in the elucidation of genetic factors which regulate cell death. 相似文献
73.
Souichi Ohta Futoshi Ono Yasuki Shiomi Teruyuki Nakao Osamu Aozasa Takatoshi Nagate Kunihiro Kitamura Shoji Yamaguchi Masatoshi Nishi Hideaki Miyata 《Journal of applied phycology》1998,10(4):349-356
Among 106 microalgae tested, the cytopathic effect (CPE) upon Vero cells of herpes simplex virus, Type 1 (HSV-1) was inhibited
by four methanol extracts of Dunaliella bioculata C-523, D. primolecta C-525, Lyngbya sp. M-9 and Lyngbya aerugineo-coerulea
M-12. The green alga, D. primolecta, had the highest anti HSV-1 activity, since 10 μg mL-1 of extract from this alga completely
inhibited the CPE. This activity was similar to that of acyclovir at the same concentration. We compared anti-viral activities
against adeno virus, herpes simplex virus-2 (HSV-2), Japanese Encephalitis and Polio viruses. Only the CPE of HSV-2 was inhibited.
Thus, the factor was specific against HSV. The antiviral activity was apparently excited during HSV adsorption and invasion
of the cells. We optimized the conditions for anti HSV-1 activity by prolonging the exposure of HSV-1 to the extract. After
2 h, the CPE of even a high titer of HSV-1 (106 TCID50/0.1 mL) was completely inactivated. By use of various chromatographic
techniques, three green substances having anti-HSV activity were purified from the algal mass of D. primolecta, and 5 μg mL-1
of this purified substances completely inhibited the CPE. From the analysis of NMR and MS, the chemical structures of the
active substances were identified as pheophorbide-like compounds.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
74.
Kouichi Takeshi Tetsuya Ikeda Akiko Kubo Yukako Fujinaga Souichi Makino Keiji Oguma Emiko Isogai Shin-ichi Yoshida Hiroyuki Sunagawa Tohru Ohyama Hiroo Kimura 《Microbiology and immunology》1997,41(10):819-822
Direct detection of Escherichia coli O157 and foodborne pathogens associated with bloody diarrhea were achieved using polymerase chain reaction (PCR) after the preparation of DNA from stool specimens using the microspin technique. PCR was compared with cultivation and toxin production tests with respect to the efficiency of detection of each pathogen; E. coli O157, Vibrio parahaemolyticus, Salmonella serovar Enteritidis and Campylobacter jejuni. Detection of some or all of the above pathogens in clinical stool specimens was achieved using PCR. The minimum number of cells required for the detection of the above pathogens by PCR was 101 CFUs/0.5 g of stool sample. PCR was completed within 6 hr. The above pathogens were also detected in cultivation and toxin production tests. Partial purification of the template DNA using the microspin technique was essential for the elimination of PCR inhibitors from the DNA samples. This PCR method is an accurate, easy-to-read screening method for the detection of Shiga-like toxin producing E. coli O157 and enteropathogens associated with bloody diarrhea in stool specimens. 相似文献
75.
Souichi Ohta Yasuki Shiomi Akira Kawashima Osamu Aozasa Teruyuki Nakao Takatoshi Nagate Kunihiro Kitamura Hideaki Miyata 《Journal of applied phycology》1995,7(2):121-127
Methanol extracts fromChlorococcum strain HS-101 andDunaliella primolecta strongly inhibited the growth of a strain of methicillin-resistantStaphylococcus aureus (MRSA), which is causing serious problems in Japanese hospitals. So that the anti-MRSA substance(s) could be purified and identified, the growth medium was improved for antibiotic production. When the two strains were cultured in their improved media, antibiotic production byChlorococcum strain HS-101 was 1.8-fold that in the standard BG-11 medium, and production byD. primolecta was 2.3-fold. The activity pattern of fractions eluted by silica-gel or gel-permeation chromatography suggested that both strains produced two antibiotic substances. Identification of the purified substances by NMR and GC-MS showed that one of the active substances in both strains was-linolenic acid. Ten fatty acids from other sources were tested, and it was found that unsaturated fatty acids had antibiotic activity against MRSA, with the highest activity that of -linolenic acid. 相似文献
76.
Souichi Nukuzuma Chiyoko Nukuzuma Masanori Kameoka Shigeki Sugiura Kazuo Nakamichi Takafumi Tasaki Tsutomu Takegami 《Microbiology and immunology》2017,61(6):232-238
77.
Akiro Okuda Masaru Kubota Machiko Sawada Seiji Koishi Akihiro Kataoka Rikimaru Bessho Ikuya Usami Ying Wei Lin Souichi Adachi Kenshi Furusho 《Journal of cellular physiology》1996,168(1):183-187
Treatment of circulating human neutrophils with recombinant human granulocyte colony-stimulating factor (rhG-CSF) for 30 min augmented superoxide generation and chemotaxis induced by N-formylmethionyl-leucyl-phenylalanine (fMLP) in a dose dependent manner. When neutrophils were treated with 1 μM of methotrexate (MTX) for 60 min after incubation with rhG-CSF (10 ng/ml), the effects of rhG-CSF on superoxide generation and chemotaxis were inhibited by approximately 49 and 29%, respectively. Although inhibitory effects of MTX were also seen in neutrophils not pretreated with rhG-CSF, the degree of inhibition was much less. The addition of either hypoxanthine or guanosine at a concentration of 100 μM to the culture medium significantly attenuated the effects of MTX. However, in neutrophils obtained from a patient with Lesch-Nyhan syndrome, which lacked hypoxanthine-guanine phosphoribosyl transferase activity, neither hypoxanthine nor guanosine had any rescue effect. These results suggest that MTX inhibits superoxide generation and chemotaxis in rhG-CSF-activated neutrophils, at least in part, by disturbing purine nucleotide biosynthesis. © 1996 Wiley-Liss, Inc. 相似文献
78.
79.
Shuetsu Usami Satoru Motoyama Souichi Koyota Kaori Hayashi-Shibuya Kiyotomi Maruyama Hajime Saito Shin Takasawa Toshihiro Sugiyama 《Biochemical and biophysical research communications》2010,392(1):4-8
Regenerating gene (REG) I plays important roles in cancer cell biology. The purpose of this study was to determine whether REG I affects cytokine production in cancer cells. We transfected TE-5 and TE-9 squamous esophageal cancer cells with REG Iα and Iβ and examined its effects on cytokine expression. We found that transfecting TE-5 and TE-9 cells with REG I Iα and Iβ led to significantly increased expression of interleukin (IL)-6 mRNA and protein, but it had little or no effect on expression of IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17A, interferon-γ, tumor necrosis factor-α, granulocyte-colony stimulating factor or transforming growth factor-β1. The elevated IL-6 expression seen in REG Iα transfectants was silenced by small interfering RNA-mediated knockdown. These finding suggest that REG I may act through IL-6 to exert effects on squamous esophageal cancer cell biology. 相似文献
80.
Kanzawa N Nishigaki K Hayashi T Ishii Y Furukawa S Niiro A Yasui F Kohara M Morita K Matsushima K Le MQ Masuda T Kannagi M 《FEBS letters》2006,580(30):6807-6812
Severe acute respiratory syndrome (SARS) is characterized by rapidly progressing respiratory failure resembling acute/adult respiratory distress syndrome (ARDS) associated with uncontrolled inflammatory responses. Here, we demonstrated that, among five accessory proteins of SARS coronavirus (SARS-CoV) tested, 3a/X1 and 7a/X4 were capable of activating nuclear factor kappa B (NF-κB) and c-Jun N-terminal kinase (JNK), and significantly enhanced interleukin 8 (IL-8) promoter activity. Furthermore, 3a/X1 and 7a/X4 expression in A549 cells enhanced production of inflammatory chemokines that were known to be up-regulated in SARS-CoV infection. Our results suggest potential involvement of 3a/X1 and 7a/X4 proteins in the pathological inflammatory responses in SARS. 相似文献