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Vijaya Bharathi D Jagadeesh B Hotha KK Patil U Bhushan I 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2011,879(26):2827-2833
A highly sensitive, selective and evaporation free SPE extraction, ESI-LC-MS/MS method has been developed for estimation of misoprostol free acid in human plasma using misoprostol acid-d(5) as an internal standard (IS). The analyte was separated using isocratic mobile phase on reverse phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M-H] anions, m/z 367-249 for misoprostol acid and m/z 372-249 for the IS. The total run time was 5.0 min and the elution of misoprostol acid and misoprostol acid-d(5) (IS) occurred at 3.6 min. The developed method was validated in human plasma with a lower limit of quantification of 2.5 pg/mL. A linear response function was established for the range of concentrations 2.5-1200 pg/mL (r>0.998) for misoprostol acid in human plasma. The intra and inter-day precision values for misoprostol acid met the acceptance as per FDA guidelines. Misoprostol acid was stable in the battery of stability studies viz., bench-top, auto-sampler and freeze/thaw cycles. The developed assay method was applied to an oral pharmacokinetic study in humans. 相似文献
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Subramaniya BR Srinivasan G Sadullah SS Davis N Subhadara LB Halagowder D Sivasitambaram ND 《PloS one》2011,6(4):e18695
Plumbagin, a quinonoid found in the plants of the Plumbaginaceae, possesses medicinal properties. In this study we investigated the anti-proliferative and apoptotic activity of plumbagin by using two human colonic cancer cell lines, HT29 and HCT15. IC50 of Plumbagin for HCT15 and HT29 cells (22.5 μM and 62.5 μM, respectively) were significantly different. To study the response of cancer cells during treatment strategies, cells were treated with two different concentrations, 15 μM, 30 μM for HCT15 and 50 μM, 75 μM for HT29 cells. Though activation of NFκB, Caspases-3, elevated levels of TNF-α, cytosolic Cytochrome C were seen in both HCT15 cells HT29 treated with plumbagin, aberrant apoptosis with decreased level of pEGFR, pAkt, pGsk-3β, PCNA and Cyclin D1was observed only in 15 μM and 30 μM plumbagin treated HCT15 and 75 μM plumbagin treated HT29 cells. This suggests that plumbagin induces apoptosis in both HCT15 cells and HT29 treated, whereas, proliferation was inhibited only in 15 μM and 30 μM plumbagin treated HCT15 and 75 μM plumbagin treated HT29 cells, but not in 50 μM plumbagin treated HT29 cells. Expression of COX-2 was decreased in 75 μM plumbagin treated HT29 cells when compared to 50 μM plumbagin treated HT29 cells, whereas HCT15 cells lack COX. Hence the observed resistance to induction of apoptosis in 50 μM plumbagin treated HT29 cells are attributed to the expression of COX-2. In conclusion, plumbagin induces apoptosis in colonic cancer cells through TNF-α mediated pathway depending on expression of COX-2 expression. 相似文献
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To evaluate the contribution of Sulfate-Reducing Bacteria (SRB) to the carbon flux from marine salterns, sulfate reducing activity (SRA) was measured for a year (Sept 2000–Aug 2001) This covered the pre-monsoon period (Feb–May), the monsoon (June–Sept) and the post-monsoon (Oct–Jan) in the salterns of Ribandar, Goa, India. Sulphate reduction was examined to gain insights into the decomposition characteristics (kinetics) of organic carbon; this connection is based on the theory of stoichiometric coupling between anaerobic carbon degradation and sulphate reduction to sulphide. SRA was measured using radiotracer techniques. Spatially, recalcitrance of the substrates, as depicted by the reaction rate coefficient (k), increased with depth, except during the monsoons. The values ranged from 0.0061 during the pre monsoon to 0.06 month?1 during the post-monsoon at 5–10 cm depth. The recalcitrance was highest at the surface during the monsoons at a k value of 0.014 month?1. The anaerobic degradation of labile organic matter followed first order kinetics or G model during non-monsoon seasons when the substrate was limiting due to closure of the system to the addition of extraneous source of organic matter. However, the integrated recalcitrance or the reaction rate coefficient was much higher during the post monsoon at 0.642 month?1 compared to 0.053 month?1 during the pre-monsoon season. Interestingly, the reaction rate followed zero-order kinetics when the substrate was non-limiting during monsoon due to exposure to external inputs. 相似文献
57.
Thirugnanam Karthikeyan Mariappan Pravin Velusamy Shanmuganathan Muthusamy Rajaganapathy Bharathi Raja Baddireddi Subhadra Lakshmi 《Probiotics and antimicrobial proteins》2013,5(1):51-58
The current study investigated the immunomodulatory potential of ethyl acetate soluble supernatant of Lactobacillus casei (LC-EAS) in vitro. The effect of LC-EAS on nitric oxide release was analyzed in RAW 264.7 cells, wherein, an inhibition in nitric oxide production through suppression of inducible nitric oxide synthase mRNA expression was observed. Evaluation of LC-EAS on LPS-induced peripheral blood mononuclear cells showed a down-regulation in TNF-α and IL-6 genes and an upregulation of IL-10. An inhibition in the protein expression of NF-κB, ERK1/2 and STAT3 phosphorylation confirms the immunomodulatory potential of LC-EAS. The effect of LC-EAS on in vitro intestinal epithelial cells was investigated using HT-29 human colon adenocarcinoma cancer cells. LC-EAS exhibited an inhibition of NF-κB and ERK1/2 phosphorylation, whereas STAT3 phosphorylation was unregulated. To evaluate the downstream target of STAT3 upregulation, expression of the intestinal trefoil factor TFF3 which is a NF-κB regulator and STAT3 downstream target was studied. LC-EAS was observed to elevate TFF3 mRNA expression. Overall the study shows that the anti-inflammatory potential of LC-EAS is through inhibition of NF-κB in different cell types. 相似文献
58.
Karunakaran E Mukherjee J Ramalingam B Biggs CA 《Applied microbiology and biotechnology》2011,90(6):1869-1881
The observation of biofilm formation is not a new phenomenon. The prevalence and significance of biofilm and aggregate formation
in various processes have encouraged extensive research in this field for more than 40 years. In this review, we highlight
techniques from different disciplines that have been used to successfully describe the extracellular, surface and intracellular
elements that are predominant in understanding biofilm formation. To reduce the complexities involved in studying biofilms,
researchers in the past have generally taken a parts-based, disciplinary specific approach to understand the different components
of biofilms in isolation from one another. Recently, a few studies have looked into combining the different techniques to
achieve a more holistic understanding of biofilms, yet this approach is still in its infancy. In order to attain a global
understanding of the processes involved in the formation of biofilms and to formulate effective biofilm control strategies,
researchers in the next decade should recognise that the study of biofilms, i.e. biofilmology, has evolved into a discipline
in its own right and that mutual cooperation between the various disciplines towards a multidisciplinary research vision is
vital in this field. 相似文献
59.
Ramakrishna S Suresh B Lee EJ Lee HJ Ahn WS Baek KH 《The Journal of biological chemistry》2011,286(12):10505-10514
SDS3 is a key component of the histone deacetylase (HDAC)-dependent Sin3A co-repressor complex, serving to maintain its HDAC activity. Here, we report both exogenous and endogenous functional interaction between deubiquitinating enzyme USP17 and human SDS3 by MALDI-TOF-MS, co-immunoprecipitation assay, and GST pull-down assay. In this study, we demonstrated that SDS3 readily undergoes endogenous polyubiquitination, which is associated specifically with Lys-63-branched polyubiquitin chains and not with Lys-48-branched polyubiquitin chains. Further, we also demonstrated that USP17 specifically deubiquitinates Lys-63-linked ubiquitin chains from SDS3 and regulates its biological functions. The deubiquitinating activity of USP17 on SDS3 negatively regulates SDS3-associated HDAC activity. The constitutive expression of USP17 and its substrate SDS3 was involved in the inhibition of anchorage-independent tumor growth and blocks cell proliferation, leading to apoptosis in cervical carcinoma cells. Furthermore, we showed that USP17 and SDS3 mutually interact with each other to regulate cancer cell viability. These data support the possibility that SDS3, being a substrate of USP17, may play an important role in developing a novel therapeutic means to inhibit specific HDAC activities in cancer. 相似文献
60.
Jiao Chen Tianhong Pan Bharathi Devi Devendran Zhankun Xi Swanand Khare Biao Huang Weiping Zhang Stephan Gabos Dorothy Yu Huang Can Jin 《Analytical biochemistry》2015
Over the past decade, the real-time cell analyzer (RTCA) has provided a good tool to the cell-based in vitro assay. Unlike the traditional systems that label the target cells with luminescence, fluorescence, or light absorption, RTCA monitors cell properties using noninvasive and label-free impedance measuring. However, realization of the maximum value of RTCA for applications will require assurance of within-experiment repeatability, day-to-day repeatability, and robustness to variations in conditions that might occur from different experiments. In this article, the performance and variability of RTCA is evaluated and a novel repeatability index (RI) is proposed to analyze the intra-/inter-E-plate repeatability of RTCA. The repeatability assay involves six cell lines and two media (water [H2O] and dimethyl sulfoxide [DMSO]). First, six cell lines are exposed to the media individually, and time-dependent cellular response curves characterized as a cell index (CI) are recorded by RTCA. Then, the variations along sampling time and among repeated tests are calculated and RI values are obtained. Finally, a discriminating standard is set up to evaluate the degree of repeatability. As opposed to the standardized methodologies, it is shown that the presented index can give the quantitative evaluation for repeatability of RTCA within E-plate and variation on different days. 相似文献