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971.
Hideaki Otsuka Toshiyuki Akiyama Ken-Ichi Kawai Shoji Shibata Osamu Inoue Yukio Ogihara 《Phytochemistry》1978,17(8):1349-1352
Stepwise chemical and enzymatic hydrolyses of jujubosides A and B, the saponins of the seeds of Zizyphus jujuba, afforded prosapogenins I, II and III. The sequence and configuration of the sugar linkages in the saponins and the pro-sapogenins were determined from the PMR data and the application of Klyne's rule of molecular rotations and structures were assigned to jujubosides A and B. 相似文献
972.
973.
Shigehisa Sato Masahiro Tsuchiya Ken-ichiro Komaki Shin-ichiro Kusunoki Shinobu Tsuchiya Naoto Haruyama Ichiro Takahashi Yasuyuki Sasano Makoto Watanabe 《Histochemistry and cell biology》2009,131(5):583-591
The expression of type I collagen, the most component of dentin extracellular matrix proteins (ECMs) in odontoblast is correlated
with the activity of dentin formation. Since odontoblast possesses a distinct cellular process for protein transport into
the dentinal tubule, it is important to examine the intracellular protein localization. However, a study focusing on odontoblast
processes has not been performed. Type I collagen is synthesized as procollagen, which is immediately converted to collagen
upon secretion. After characterization of antiserum to rat type I procollagen, we investigated the intracellular localization
of type I procollagen in odontoblasts during and after dentinogenesis, using immunohistochemistry and in situ hybridization.
The level of mRNA expression decreased during dentinogenesis, whereas the intracellular localization of type I procollagen
in odontoblast processes become more distinct. The percentage of dentinal tubules with type I procollagen increased significantly
with aging. Odontoblasts in pulp horn, in particular, showed moderate expression of type I procollagen after dentinogenesis.
Since loss of occlusion also caused a significant decrease in type I procollagen, we concluded that occlusal stimulation activated
type I procollagen synthesis in odontoblasts. We also suggest that analysis of intracellular transport of type I procollagen
via odontoblast processes may be a new approach to evaluation of odontoblast function. 相似文献
974.
Akira Kikuchi Masashi Asahina Motoki Tanaka Shinobu Satoh Hiroshi Kamada 《Journal of plant research》2013,126(2):243-250
Totipotency is the ability of a cell to regenerate the entire organism, even after previous differentiation as a specific cell. When totipotency is coupled with active cell division, it was presumed that cell division is essential for this expression. Here, using the stress-induction system of somatic embryos in carrots, we show that cell division is not essential for the expression of totipotency in somatic/embryonic conversion. Morphological and histochemical analyses showed that the cell did not divide during embryo induction. Inhibitors of cell division did not affect the rate of somatic embryo formation. Our results indicate that the newly acquired trait of differentiation appears without cell division, but does not arise with cell division as a newborn cell. 相似文献
975.
A pair of leaflets excised from Mimosa pinna and floated onwater for a day, responded to light and mechanical stimuli.Only when a small spot of light was shone on the abaxial surfaceof pulvinule, did the opening response of the leaflets occur.No translocatable effect from suchirradiation was observed.
1 Present address: Biological Institute, Faculty of Science,Kobe University, Kobe 657, Japan. (Received May 17, 1973; ) 相似文献
976.
Yamakawa Seiyei; Watanabe Masao; Isogai Akira; Takayama Seiji; Satoh Shinobu; Hinata Kokichi; Suzuki Akinori 《Plant & cell physiology》1993,34(1):173-175
A cDNA clone encoding NS3-glycoprotein was isolated from stigmasof Brassica campestris. NS-glycoproteins correspond to the SLRI-glycoproteinsof B. oleracea and are highly conserved within the species.These data suggest that the NS-glycoproteins may play a rolein discrimination between species in the fertilization systemof Brassica. (Received September 4, 1992; Accepted November 9, 1992) 相似文献
977.
Ogihara Mari H. Yoshiyama Mikio Morimoto Nobuo Kimura Kiyoshi 《Applied Entomology and Zoology》2020,55(2):189-197
Applied Entomology and Zoology - Varroa destructor Anderson and Trueman (Acari: Varroidae) are ectoparasitic mites found in the western honeybee Apis mellifera Linnaeus (Hymenoptera: Apidae).... 相似文献
978.
979.
980.
Shinobu Sueyoshi Hitomi Nagakura Akira Kato Setsuyoshi Uetsuki Yasuo Nakayama Masakazu Adachi 《Glycoconjugate journal》1992,9(2):99-108
The antigen structure of a mouse monoclonal antibody, GOM-2, established by immunization with KATO-III human gastric cancer cells, was examined. GOM-2 reactive glycolipids were prepared from KATO-III cells and treated with endoglycoceramidase. Structural studies of ten GOM-2 reactive oligosaccharides by a combination of glycosidase digestions, methylation, and affinity chromatography on anUlex europeus agglutinin I (UEA-I) column revealed that nine of them had a Y-related B-active difucosylated determinant (B-Ley) and one had a B-active determinant. Affinity chromatography of the purified and modified oligosaccharides on an immobilized GOM-2 column demonstrated that GOM-2 has a novel binding specificity; it binds tightly to the biantennary structure carrying the B-Ley determinant at the termini or the branched structure carrying the B-Ley structure at two nonreducing termini.Abbreviations UEA-I
Ulex europeus agglutinin I
- PNA
Arachis hypogaea agglutinin
- Fuc
l-fucose
- Gal
d-galactose
- Glcol
glucitol
- GlcNAc
N-acetyl-d-glucosamine
- TBS
10mm Tris-HCl, pH 7.8, containing 150mm NaCl
- PBS
10mm sodium phosphate buffer, pH 7.5, containing 150mm NaCl
- HPLC
high performance liquid chromatography. 相似文献