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31.
Eizo Sada Shigeo Katoh Masami Shiozawa Tsunehiko Fukui 《Biotechnology and bioengineering》1981,23(11):2561-2567
The performance of fluidized-bed reactors utilizing a magnetic field was determined by the use of magnetite-containing beads of immobilized unease. The reactors showed similar or higher conversions in comparison with fixed-bed reactors, although some aggregation of the beads in the magnetic field was observed. No effusion of the beads occurred up to a flow rate of 24 cm/min. 相似文献
32.
Shigeo Katoh Tomio Kambayashi Ryuichi Deguchi Fumitake Yoshida 《Biotechnology and bioengineering》1978,20(2):267-280
Performance of affinity chromatography columns was studied by measuring the rates of adsorption and elution of trypsin in a Sepharose 4B-soybean trypsin inhibitor column and a Sepharose 4B-arginine peptides column. The volumetric coefficient for trypsin transfer was evaluated from the break-through curves of trypsin, and elution profiles bed height of Sepharose 4B-STI column was estimated based on these results. 相似文献
33.
The neutral component of d-mannan of bakers' yeast (Saccharomyces cerevisiae), consisting solely of d-mannose residues, was precipitated with concanavalin A to give four fractions. The first three displayed similar reactivities in quantitative precipitin reaction against concanavalin A and homologous anti-S. cerevisiae serum, but the fourth showed different precipitin curves. Analysis of the fractions by acetolysis indicated structural differences. The different behavior of the last fraction in precipitin reactions could be due to a lower content of branching points, or to shorter chain-lengths. 相似文献
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35.
H. Ueda Takeshi Baba Nobuo Terada Yasuko Kato Shigeo Tsukahara Shinichi Ohno 《Histochemistry and cell biology》1997,108(3):243-248
It is known that the retina contains the protein dystrophin in the ribbon synapse, but the ultrastructural analysis is not
yet fully elucidated. Our previous study reported that dystrophin is localized under the rod cell membranes in rat retinas.
In the present study, we have investigated the relationship between dystrophin-rich regions of rod cell membranes and other
neuronal processes in mouse retinas with a monoclonal antibody raised against the human dystrophin C-terminus. Immunoblotting,
immunofluorescence stainings, and immunoelectron microscopy were employed. Immunoblotting analysis indicated that mouse retinas
possessed some of the dystrophin isoforms of approximately 260 kDa, 140 kDa, and 70 kDa molecular weight. Confocal images
showed a punctate appearance in the outer plexiform layer, as previously described. Immunoelectron microscopy showed that
dystrophin immunoreactive products were always observed at submembranous dense regions of the rod spherule abutting bipolar
processes. These results suggest that retinal dystrophin may be closely involved in signal transmission from rods to bipolar
cells.
Accepted: 7 May 1997 相似文献
36.
Shizuya Tanaka Toshiro Kato Shigeo Yamamoto Hirosuke Yoshioka 《Bioscience, biotechnology, and biochemistry》2013,77(10):1953-1959
Since S-n-butyl S′-p-tert-butylbenzyl N-3-pyridyldithiocarbonimidate, a potent fungicide to powdery mildew, is known to inhibit ergosterol biosynthesis in Monilinia fructigena, the activities were assessed on 24 compounds having other substituents than the 3-pyridyl and on 24 compounds having a variety of different structures connecting the 3-pyridyl and the p-tert-butylphenyl group from that of the dithiocarbonimidate.In the former group the 3-pyridyl group was essential for the activities and the substitution at the 2- or 6-position resulted, on available data, in inactive compounds. Several other β-N-heterocyclic analogs were also active. In the latter group, a number of modified compounds from the dithiocarbonimidate structure were shown to be active. 相似文献
37.
Masuko Suzuki Takeshi Mikami Tatsuji Matsumoto Shigeo Suzuki 《Microbiology and immunology》1977,21(8):419-425
The Limulus test has been considered specific for the presence of bacterial endotoxins. To synthesize a simple model of endotoxin, palmitoyldextran phosphate was prepared by modification of dextran by palmitoylation and phosphorylation. The present studies indicated that a variety of polysaccharide derivatives, such as palmitoyldextran phosphate, palmitoyldextran, and dextran phosphate, give a positive Limulus test and show pyrogenic activity, except for low molecular dextran derivatives. On the other hand, polysaccharides, such as dextran, starch (soluble), chitosan, xylan, and lentinan, were negative in these assays. The gelation reaction of Limulus lysate by modified dextran derivatives may depend on the molecular weight or modification of polysaccharides by palmitoylation and/or phosphorylation to a great extent. 相似文献
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40.
Tomoyuki Yamanaka Asako Tosaki Masaru Kurosawa Kazunori Akimoto Tomonori Hirose Shigeo Ohno Nobutaka Hattori Nobuyuki Nukina 《PloS one》2013,8(12)
Cell polarity plays a critical role in neuronal differentiation during development of the central nervous system (CNS). Recent studies have established the significance of atypical protein kinase C (aPKC) and its interacting partners, which include PAR-3, PAR-6 and Lgl, in regulating cell polarization during neuronal differentiation. However, their roles in neuronal maintenance after CNS development remain unclear. Here we performed conditional deletion of aPKCλ, a major aPKC isoform in the brain, in differentiated neurons of mice by camk2a-cre or synapsinI-cre mediated gene targeting. We found significant reduction of aPKCλ and total aPKCs in the adult mouse brains. The aPKCλ deletion also reduced PAR-6β, possibly by its destabilization, whereas expression of other related proteins such as PAR-3 and Lgl-1 was unaffected. Biochemical analyses suggested that a significant fraction of aPKCλ formed a protein complex with PAR-6β and Lgl-1 in the brain lysates, which was disrupted by the aPKCλ deletion. Notably, the aPKCλ deletion mice did not show apparent cell loss/degeneration in the brain. In addition, neuronal orientation/distribution seemed to be unaffected. Thus, despite the polarity complex disruption, neuronal deletion of aPKCλ does not induce obvious cell loss or disorientation in mouse brains after cell differentiation. 相似文献