全文获取类型
收费全文 | 113篇 |
免费 | 14篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2015年 | 5篇 |
2014年 | 5篇 |
2013年 | 4篇 |
2012年 | 4篇 |
2011年 | 8篇 |
2010年 | 5篇 |
2009年 | 12篇 |
2008年 | 9篇 |
2007年 | 12篇 |
2006年 | 10篇 |
2005年 | 5篇 |
2004年 | 6篇 |
2003年 | 6篇 |
2002年 | 4篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有127条查询结果,搜索用时 15 毫秒
121.
A marked reduction in uptake of α-santonin, accompanied by loss of ability of cells to transform the substrate, is observed
on shocking Sphingomonas paucimobilis strain S ATCC 43388 cells by freeze — thaw method. The shock fluid shows a 26% quench in fluorescence at 350nm on incubation
with the substrate. Addition of shock fluid to the freeze thawed cells restores both uptake as well as transformation of α-santonin
to near normal. 相似文献
122.
123.
A conserved domain of the large subunit of replication factor C binds PCNA and acts like a dominant negative inhibitor of DNA replication in mammalian cells. 总被引:4,自引:0,他引:4 下载免费PDF全文
R Fotedar R Mossi P Fitzgerald T Rousselle G Maga H Brickner H Messier S Kasibhatla U Hübscher A Fotedar 《The EMBO journal》1996,15(16):4423-4433
Replication factor C (RF-C), a complex of five polypeptides, is essential for cell-free SV40 origin-dependent DNA replication and viability in yeast. The cDNA encoding the large subunit of human RF-C (RF-Cp145) was cloned in a Southwestern screen. Using deletion mutants of RF-Cp145 we have mapped the DNA binding domain of RF-Cp145 to amino acid residues 369-480. This domain is conserved among both prokaryotic DNA ligases and eukaryotic poly(ADP-ribose) polymerases and is absent in other subunits of RF-C. The PCNA binding domain maps to amino acid residues 481-728 and is conserved in all five subunits of RF-C. The PCNA binding domain of RF-Cp145 inhibits several functions of RF-C, such as: (i) in vitro DNA replication of SV40 origin-containing DNA; (ii) RF-C-dependent loading of PCNA onto DNA; and (iii) RF-C-dependent DNA elongation. The PCNA binding domain of RF-Cp145 localizes to the nucleus and inhibits DNA synthesis in transfected mammalian cells. In contrast, the DNA binding domain of RF-Cp145 does not inhibit DNA synthesis in vitro or in vivo. We therefore conclude that amino acid residues 481-728 of human RF-Cp145 are critical and act as a dominant negative mutant of RF-C function in DNA replication in vivo. 相似文献
124.
125.
Aayeti Shailaja Mote Srinath Byreddi Venkata Bhavani Bindu Charu Chandra Giri 《Physiology and Molecular Biology of Plants》2021,27(2):223
The full length Andrographis paniculate 4-hydroxy 3-methyl 2-butenyl 4-diphosphate reductase (ApHDR) gene of MEP pathway was isolated for the first time. The ApHDR ORF with 1404 bp flanked by 100 bp 5′UTR and 235 bp 3′UTR encoding 467 amino acids (NCBI accession number: ) and cloned in pET 102, transformed and expressed in E. coli BL21. The ApHDR protein physico-chemical properties, secondary and tertiary structure were analyzed. The Ramachandran plot showed 93.8% amino acids in the allowed regions, suggesting high reliability. The cluster of 16 ligands for binding site in ApHDR involved six amino acid residues having 5–8 ligands. The Fe-S cluster binding site was formed with three conserved residues of cysteine at positions C123, C214, C251 of ApHDR. The substrate HMBPP and inhibitors clomazone, paraquat, benzyl viologen’s interactions with ApHDR were also assessed using docking. The affinity of Fe-S cluster binding to the cleft was found similar to HMBPP. The HPLC analysis of different type of tissue (plant parts) revealed highest andrographolide content in young leaves followed by mature leaves, stems and roots. The differential expression profile of ApHDR suggested a significant variation in the expression pattern among different tissues such as mature leaves, young leaves, stem and roots. A 16-fold higher expression of ApHDR was observed in the mature leaves of A. paniculata as compared to roots. The young leaves and stem showed 5.5 fold and fourfold higher expression than roots of A. paniculata. Our result generated new genomic information on ApHDR which may open up prospects of manipulation for enhanced diterpene lactone andrographolide production in A. paniculata.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12298-021-00952-0. MK503970相似文献
126.
Russian Journal of Bioorganic Chemistry - A series of Schiff base conjugate of isatin with 2-thiopheneethylamine and its Mannich bases 3?(2-(thiophen-2-yl) ethylimino)indolin-2-ones... 相似文献
127.
Florent Angly Merry Youle Bahador Nosrat Shailaja Srinagesh Beltran Rodriguez-Brito Patrick McNairnie Gordafaried Deyanat-Yazdi Mya Breitbart Forest Rohwer 《Environmental microbiology》2009,11(11):2863-2873
Roseophage SIO1 is a lytic marine phage that infects Roseobacter SIO67, a member of the Roseobacter clade of near-shore alphaproteobacteria. Roseophage SIO1 was first isolated in 1989 and sequenced in 2000. We have re-sequenced and re-annotated the original isolate. Our current annotation could only assign functions to seven additional open reading frames, indicating that, despite the advances in bioinformatics tools and increased genomic resources, we are still far from being able to translate phage genomic sequences into biological functions. In 2001, we isolated four new strains of Roseophage SIO1 from California near-shore locations. The genomes of all four were sequenced and compared against the original Roseophage SIO1 isolated in 1989. A high degree of conservation was evident across all five genomes; comparisons at the nucleotide level yielded an average 97% identity. The observed differences were clustered in protein-encoding regions and were mostly synonymous. The one strain that was found to possess an expanded host range also showed notable changes in putative tail protein-coding regions. Despite the possibly rapid evolution of phage and the mostly uncharacterized diversity found in viral metagenomic data sets, these findings indicate that viral genomes such as the genome of SIO1-like Roseophages can be stably maintained over ecologically significant time and distance (i.e. over a decade and ∼50 km). 相似文献