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71.
In six liquid culture media, all of which stimulated Candida albicans to grow in the hyphal form, the rates of hyphal extension and increase in cellular ATP concentration, hyphal diameters, times of evagination of hyphae, times of septum formation and positions of septa in the hyphae appeared to vary independently. There were no discernible associations between properties such as length or volume of hyphal compartments at the time of septation and temporal parameters of hyphal growth. The results suggest that growth environment influences many of the processes contributing to hyphal development, but that these processes are not necessarily interrelated. 相似文献
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74.
Growth and macromolecular content of the dimorphic fungus Aureobasidium pullulans and the effect of hydroxyurea and other inhibitors 总被引:1,自引:0,他引:1
The growth kinetics and the macromolecular content of the yeast and ethanol-induced hyphal forms of Aureobasidium pullulans were studied. During the morphological transition from yeasts to hyphae, both the protein and RNA content decreased significantly, the mycelial form containing only 76% of the amount of protein in the yeasts, and 38% of the RNA. The DNA was the only component tested whose level increased during the transition. Among several compounds inhibiting macromolecular synthesis, only hydroxyurea showed a remarkable effect on the morphology of A. pullulans, inducing the mycelial morphology. The macromolecular composition of hydroxyurea-treated cultures changed with time in a way similar to that of the ethanol-Tween 80-ammonia medium, and to that of carbon-starved cultures, without ethanol or glucose. 相似文献
75.
76.
Sánchez Fernández JF González-López CV Acién Fernández FG Fernández Sevilla JM Molina Grima E 《Applied microbiology and biotechnology》2012,94(3):613-624
This paper focuses on modelling the growth rate and exopolysaccharides production of Anabaena sp. ATCC 33047, to be used in carbon dioxide removal and biofuels production. For this, the influence of dilution rate, irradiance
and aeration rate on the biomass and exopolysaccharides productivity, as well as on the CO2 fixation rate, have been studied. The productivity of the cultures was maximum at the highest irradiance and dilution rate
assayed, resulting to 0.5 gbio l−1 day−1 and 0.2 geps l−1 day−1, and the CO2 fixation rate measured was 1.0 gCO2 l−1 day−1. The results showed that although Anabaena sp. was partially photo-inhibited at irradiances higher than 1,300 μE m−2 s−1, its growth rate increases hyperbolically with the average irradiance inside the culture, and so does the specific exopolysaccharides
production rate. The latter, on the other hand, decreases under high external irradiances, indicating that the exopolysaccharides
metabolism hindered by photo-damage. Mathematical models that consider these phenomena have been proposed. Regarding aeration,
the yield of the cultures decreased at rates over 0.5 v/v/min or when shear rates were higher than 60 s−1, demonstrating the existence of thus existence of stress damage by aeration. The behaviour of the cultures has been verified
outdoors in a pilot-scale airlift tubular photobioreactor. From this study it is concluded that Anabaena sp. is highly recommended to transform CO2 into valuable products as has been proved capable of metabolizing carbon dioxide at rates of 1.2 gCO2 l−1 day−1 outdoors. The adequacy of the proposed equations is demonstrated, resulting to a useful tool in the design and operation
of photobioreactors using this strain. 相似文献
77.
del Olmo E Barboza B Ybarra MI López-Pérez JL Carrón R Sevilla MA Boselli C San Feliciano A 《Bioorganic & medicinal chemistry letters》2006,16(10):2786-2790
Several series of dihydrostilbenamide, imidazo[2,1-a]isoindole, pyrimido[2,1-a]isoindole and phthalazinone derivatives were obtained and their vasorelaxant activity was measured on isolated rat aorta rings pre-contracted with phenylephrine (10(-5)M). Some phthalazinones attained, practically, the total relaxation of the organ at micromolar concentrations. For the most potent compound 9h (EC(50)=0.43microM) the affinities for alpha(1A), alpha(1B) and alpha(1D) adrenergic sub-receptors were determined. 相似文献
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79.
Nader Ben Amor Wided Megdiche Ana Jiménez Francisca Sevilla Chedly Abdelly 《Acta Physiologiae Plantarum》2010,32(3):453-461
The purpose of the current investigation was to study the effect of Ca2+ (0, 3.5 and 20 mM concentrations) on the antioxidant systems in the halophyte Cakile maritima under NaCl stress (0, 100, 200 and 400 mM NaCl). Plants treated with both moderate calcium (3.5 mM) and salt levels (100 mM)
showed the maximum growth, and the addition of 20 mM calcium to the nutrient media did not significantly reduce the growth
under the moderate salt treatment. The absence of calcium associated with high salt concentration induced a strong reduction
of biomass production. The tolerance of C. maritima at moderate salinity and calcium was related with the lowest values of the parameters indicative of oxidative stress (malondialdehyde,
electrolyte leakage and hydrogen peroxide concentration). This was accompanied with a higher peroxidase, superoxide dismutase,
ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase activities. In
contrast, in the absence of calcium, those enzymes showed the lowest activities under all salt treatments. As a whole, it
can be noticed that salt tolerance was improved by moderate calcium concentration; however, the absence of calcium has a drastic
effect on C. maritima. 相似文献
80.
Barea PL Calvo E Rodriguez JA Rementeria A Calcedo R Sevilla MJ Pontón J Hernando FL 《FEMS immunology and medical microbiology》1999,23(4):343-354
The use of a two-dimensional polyacrylamide gel electrophoresis joined with Western blotting allowed us to investigate the reactivities of antibodies present in sera from mice and humans to antigens of Candida albicans blastoconidia. The analysis of the antibody response in the two models studied and the comparison between the antibody response in infected and noninfected individuals showed that the infection by C. albicans produces changes in the antibody response which may be of relevance in the serodiagnosis of invasive candidiasis. These changes include the induction of antibodies against new antigens, the disappearance of antibodies against a group of antigens and variations in the reactivity of antibodies directed to a different group of antigens. The technique used resolved the isoforms of several antigens including enolase. It is concluded that the antibody response in humans and mice with candidiasis is not homogeneously directed to all the isoforms of an antigen. 相似文献