全文获取类型
收费全文 | 276篇 |
免费 | 15篇 |
出版年
2021年 | 2篇 |
2020年 | 2篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 3篇 |
2014年 | 9篇 |
2013年 | 50篇 |
2012年 | 14篇 |
2011年 | 17篇 |
2010年 | 6篇 |
2009年 | 3篇 |
2008年 | 13篇 |
2007年 | 9篇 |
2006年 | 5篇 |
2005年 | 9篇 |
2004年 | 9篇 |
2003年 | 7篇 |
2002年 | 8篇 |
2001年 | 7篇 |
2000年 | 7篇 |
1999年 | 7篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 8篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 5篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1979年 | 6篇 |
1978年 | 2篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 1篇 |
1971年 | 2篇 |
1969年 | 1篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1965年 | 2篇 |
1962年 | 1篇 |
排序方式: 共有291条查询结果,搜索用时 281 毫秒
111.
Kimura T Horibe T Sakamoto C Shitara Y Fujiwara F Komiya T Yamamoto A Hayano T Takahashi N Kikuchi M 《Journal of biochemistry》2008,144(2):187-196
This report demonstrates for the first time that P5, a member of the protein disulphide isomerase (PDI) family, is present in the mitochondria. Various organelles were screened for proteins bearing the CGHC motif using an affinity column conjugated with the phage antibody 5E, which cross-reacts with PDI family proteins. P5 was found in bovine liver mitochondrial extract and identified by Western blot analysis using anti-P5 antibody and by mass spectrometric analysis. Results of cell fractionation, proteinase sensitivity experiments and immuno-electron microscopy supported the mitochondrial localization of P5 and also indicated the presence of ERp57, another PDI family protein, in mitochondria. Our findings will be useful for the elucidation of the translocation mechanism of PDI family proteins and their roles in mitochondria. 相似文献
112.
113.
Wnt signal transduction pathways 总被引:5,自引:0,他引:5
The Wnt signaling pathway is an ancient and evolutionarily conserved pathway that regulates crucial aspects of cell fate determination, cell migration, cell polarity, neural patterning and organogenesis during embryonic development. The Wnts are secreted glycoproteins and comprise a large family of nineteen proteins in humans hinting to a daunting complexity of signaling regulation, function and biological output. To date major signaling branches downstream of the Fz receptor have been identified including a canonical or Wnt/β-catenin dependent pathway and the non-canonical or β-catenin-independent pathway which can be further divided into the Planar Cell Polarity and the Wnt/Ca2+ pathways, and these branches are being actively dissected at the molecular and biochemical levels. In this review, we will summarize the most recent advances in our understanding of these Wnt signaling pathways and the role of these pathways in regulating key events during embryonic patterning and morphogenesis.Key words: Wnt, frizzled, dishevelled, canonical, non-canonical, β-catenin, Planar Cell Polarity 相似文献
114.
A microsatellite-enriched genomic library was obtained for the Japanese gudgeon Sarcocheilichthys variegatus microoculus, and 15 dinucleotide markers were successfully isolated and characterized. These markers were also available for other Japanese congeners, Sarcocheilichthys variegatus variegatus and Sarcocheilichthys biwaensis. In three populations of Sarcocheilichthys from Lake Biwa and the Ashida River in western Japan, seven to 27 alleles were observed for each locus. Linkage equilibrium was observed among most loci, and only one locus showed significant deviation from Hardy-Weinberg equilibrium in a population. These microsatellite markers will be useful for studies of the genetic structure of the Japanese gudgeons. 相似文献
115.
Influence of radiofrequency–electromagnetic waves from 3rd‐generation cellular phones on fertilization and embryo development in mice 下载免费PDF全文
116.
M Yokouchi T Kondo A Sanjay A Houghton A Yoshimura S Komiya H Zhang R Baron 《The Journal of biological chemistry》2001,276(37):35185-35193
The protooncogene c-Cbl has recently emerged as an E3 ubiquitin ligase for activated receptor tyrosine kinases. We report here that c-Cbl also mediates the ubiquitination of another protooncogene, the non-receptor tyrosine kinase c-Src, as well as of itself. The c-Cbl-dependent ubiquitination of Src and c-Cbl requires c-Cbl's RING finger, Src kinase activity, and c-Cbl's tyrosine phosphorylation, probably on Tyr-371. In vitro, c-Cbl forms a stable complex with the ubiquitin-conjugating enzyme UbcH7, but active Src destabilizes this interaction. In contrast, Src inhibition stabilizes the c-Cbl. UbcH7.Src complex. Finally, c-Cbl reduces v-Src protein levels and suppresses v-Src-induced STAT3 activation. Thus, in addition to mediating the ubiquitination of activated receptor tyrosine kinases, c-Cbl also acts as a ubiquitin ligase for the non-receptor tyrosine kinase Src, thereby down-regulating Src. 相似文献
117.
Takashi Komiya Shunro Kawakishi Hiroo Aoki Mitsuo Namiki 《Bioscience, biotechnology, and biochemistry》2013,77(3):349-356
In relation to the bactericidal action of γ-irradiated halogenophenol solution, radiolysis of p-bromophenol in the aqueous system under anaerobic condition was investigated. Several phenolic compounds could be isolated from the radiolysis products, and three of them were elucidated for their structures. One of them was hydroquinone and the other two were assigned as new asymmetrical dimers, 5-bromo-2,4′-dihydroxybiphenyl and 2-bromo-5,4′-dihydroxybiphenyl, which were confirmed by the syntheses of their methyl ethers. 相似文献
118.
119.
120.
Abstract: The growth cone is responsible for axonal elongation and pathfinding by responding to various modulators for neurite growth, including neurotransmitters, although the sensor mechanisms are not fully understood. Among neurotransmitters, GABA is most likely to demonstrate activity in vivo because GABA and the GABAA receptor appear even in early stages of CNS development. We investigated the GABAA receptor-mediated signaling pathway in the growth cone using isolated growth cones (IGCs). Both the GABAA binding site and the benzodiazepine modulatory site were enriched in the growth cone membrane. In the intact IGC, GABA induced picrotoxin-sensitive Cl− flux (not influx but efflux) and increased the intracellular Ca2+ concentration in a picrotoxin- and verapamil-sensitive manner. Protein kinase C (PKC)-dependent phosphorylation of two proteins identified as GAP-43 and MARCKS protein was enhanced in the intact IGC stimulated by GABA, resulting in the release of MARCKS protein and GAP-43 from the membrane. Collectively, our results suggest the following scheme: activation of the functional GABAA receptor localized in the growth cone membrane → Cl− efflux induction through the GABAA -associated Cl− channel → Ca2+ influx through an L-type voltage-sensitive Ca2+ channel → Ca2+ -dependent phosphorylation of GAP-43 and MARCKS protein by PKC. 相似文献