Seasonal dynamics and profiles of soil NO concentrations in a temperate forest

Plant and Soil - Soils are known to be significant sources of atmospheric nitric oxide (NO), a key compound in atmospheric chemistry. NO is a key regulating substance for inter- and intra-species...     

Effect of the magnetic field curvature on magnetic islands in tokamaks

The effect of the magnetic field curvature on magnetic islands in a tokamak is analyzed. It is demonstrated that the original investigation of this effect by Kotschenreuther et al. (1985) is inconsistent: on the one hand, the authors made the correct assumption that this is an ideal effect and, on the other hand, they described it in terms of the parameters characteristic of the “resistive ordering” approach, which is incompatible with the ideal approximation. More recent studies of the magnetic curvature effect have produced further ambiguities; as a result, a branch of the theory of magnetic islands has arisen that is based on the supposition that the effect under discussion can be described in terms of the Glasser-Greene-Johnson parameter D_R. This branch is shown to be erroneous, because the parameter D_R describes the plasma response to magnetic field perturbations on spatial scales of about the dimension of the linear resistive layer, while the characteristic spatial scale of the magnetic islands is much longer. It is concluded that the correct theory developed here for the magnetic curvature effect makes more optimistic predictions about its stabilizing role.     

Viable mutant homozygotes at Adh1 locus in sugar beet and their combination ablility

Selection for an increased frequency of mutant semilethal allele Adh1-S of the gene for alcohol dehydrogenase (ADH) was conducted in inbred families of sugar beet. Starting from the fourth generation, viable plants of mutant Adh1-SS homozygotes appeared. In the sixth generation of selection, the combinative ability of mutant homozygotes SS, normal homozygotes FF, and heterozygotes FS was estimated. The hybrids of mutant homozygotes outperformed the hybrids of normal homozygotes in all parameters examined (germinating capacity of seeds, length and weight of 1-week shoots, chlorophyll content in leaves, and root weight). The hybrids of heterozygotes had intermediate values of the parameters. The results obtained are discussed with regard to the mechanisms underlying the recovery of viability of mutant homozygotes and the formation of a compensating gene complex (CGC).     

Hippocampus morphology in tissue culture]

Explants of the hippocampus of newborn rats were studied neurohistologically and with electron microscope within 5--35 days of explantation. Two zones are found in the culture of the hippocampus: a zone of explant, and a zone of outgrowth. Neurons, glial cells and a network of their fibres are compactly arranged in the center of the former, whereas, the latter involves a layer of migrated glial cells. The explant is surrounded by glia limiting cells. Three types of neurons are identified in the long living culture of the hippocampus: pyramidal, polymorphic and granule cells. Numerous nerve endings observed in the hippocampic explant can be recognized as axodendritic, axosomatic and glomerular synapses. The availability of several types of neurons, a variety of synapses and their complication during outgrowth of the culture are suggestive of a formation in the hippocampic explant of a functional reflex activity.     

In vivo functions of mitogen-activated protein kinases: conclusions from knock-in and knock-out mice

Multicellular organisms achieve intercellular communication by means of signalling molecules whose effect on the target cell is mediated by signal transduction pathways. Such pathways relay, amplify and integrate signals to elicit appropriate biological responses. Protein kinases form crucial intermediate components of numerous signalling pathways. One group of protein kinases, the mitogen-activated protein kinases (MAP kinases) are kinases involved in signalling pathways that respond primarily to mitogens and stress stimuli. In vitro studies revealed that the MAP kinases are implicated in several cellular processes, including cell division, differentiation, cell survival/apoptosis, gene expression, motility and metabolism. As such, dysfunction of specific MAP kinases is associated with diseases such as cancer and immunological disorders. However, the genuine in vivo functions of many MAP kinases remain elusive. Genetically modified mouse models deficient in a specific MAP kinase or expressing a constitutive active or a dominant negative variant of a particular MAP kinase offer valuable tools for elucidating the biological role of these protein kinases. In this review, we focus on the current status of MAP kinase knock-in and knock-out mouse models and their phenotypes. Moreover, examples of the application of MAP kinase transgenic mice for validating therapeutic properties of specific MAP kinase inhibitors, and for investigating the role of MAP kinase in pathogen-host interactions will be discussed.     

Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics

In this study, a novel phase-sensitive surface plasmon resonance (SPR) setup, based on temporal modulation of a pumping beam by a photoelastic modulator, and subsequent extraction of phase information at the second and the third harmonics of the modulation frequency, has been developed to study biomolecular interactions on SPR-supporting gold. We demonstrated that the design setup provides ultra-high phase sensitivity, together with a wide dynamic range of measurements. In particular, the proposed scheme was used to study real-time interaction of biotin-protein and streptavidin-BSA complexes. We have found that the proposed technique has a detection limit as high as 2.89 x 10(-7) in terms of refractive index units (RIU). In terms of biosensing performance, a detection sensitivity of 1.3 nM from the streptavidin-maleimide/thiolated BSA complex binding reaction has also been demonstrated.