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31.
32.
Qingping?Yao Dai-Wu?Seol Zhibao?Mi Paul?D?RobbinsEmail author 《Arthritis research & therapy》2005,8(1):R16
We demonstrated previously that local, intra-articular injection of an adenoviral vector expressing human tumor necrosis factor-related
apoptosis-inducing ligand (TRAIL) in a rabbit knee model of inflammatory arthritis stimulated synovial apoptosis and reduced
inflammation. To examine whether intra-articular injection of recombinant chimeric human TRAIL protein (rTRAIL) also induces
apoptosis of proliferating rabbit synovium and reduces inflammation, we used an experimental rabbit arthritis model of rheumatoid
arthritis, induced by intra-articular introduction of allogeneic fibroblasts genetically engineered to secrete human IL-1β.
Analysis of synovium isolated from the rabbits treated with intra-articular injection of rTRAIL, relative to saline control,
showed areas of extensive acellular debris and large fibrous regions devoid of intact cells, similar to adenoviral mediated
TRAIL gene transfer. Extensive apoptosis of the synovial lining was demonstrated using TUNEL analysis of the sections, corresponding
to the microscopic findings in hematoxylin and eosin staining. In addition, leukocyte infiltration into the synovial fluid
of the inflamed knee joints following rTRAIL treatment was reduced more than 50% compared with the saline control. Analysis
of the glycosaminoglycan synthetic rate by cultured cartilage using radiolabeled sulfur and cartilage histology demonstrated
that rTRAIL did not adversely affect cartilage metabolism and structure. Analysis of serum alanine aminotransferase showed
that intra-articular injection of rTRAIL did not have adverse effects on hepatic function. These results demonstrate that
intra-articular injection of rTRAIL could be therapeutic for treating pathologies associated with rheumatoid arthritis. 相似文献
33.
34.
Kim YS Kim JM Jung DL Kang JE Lee S Kim JS Seol W Shin HC Kwon HS Van Lint C Hernandez N Hur MW 《The Journal of biological chemistry》2005,280(22):21545-21552
35.
We investigated the potential use of a spectral surface plasmon resonance (SPR) biosensor in a high-throughput analysis of mumps virus and a mumps virus-specific mAb on the arrays of a cationic polyelectrolyte, poly(diallyldimethylammonium chloride) (PDDA). The PDDA surface was constructed by electrostatic adsorption of the polyelectrolyte onto a monolayer of 11-mercaptoundecanoic acid (MUA). Poly-L-lysine was also adsorbed onto the MUA monolayer and compared with the PDDA surface in the capacity of mumps virus immobilization. The PDDA surface showed a higher adsorption of mumps virus than the poly-L-lysine surface. The SPR signal caused by the virus binding onto the PDDA surface was proportional to the concentration of mumps virus from 0.5 x 10(5) to 14 x 10(5) pfu/mL. The surface structure of the virus arrays was visualized by atomic force microscopy. Then, a dose-dependent increase in the SPR signal was observed when various concentrations of the antimumps virus antibody in buffer or human serum were applied to the virus arrays, and their interaction was specific. Thus, it is likely that the spectral SPR biosensor based on the cationic polyelectrolyte surface may provide an efficient system for a high-throughput analysis of intact virus and serodiagnosis of infectious diseases. 相似文献
36.
The Rey-Osterrieth Complex Figure Test (ROCF), which was developed by Rey in 1941 and standardized by Osterrieth in 1944, is a widely used neuropsychological test for the evaluation of visuospatial constructional ability and visual memory. Recently, the ROCF has been a useful tool for measuring executive function that is mediated by the prefrontal lobe. The ROCF consists of three test conditions: Copy, Immediate Recall and Delayed Recall. At the first step, subjects are given the ROCF stimulus card, and then asked to draw the same figure. Subsequently, they are instructed to draw what they remembered. Then, after a delay of 30 min, they are required to draw the same figure once again. The anticipated results vary according to the scoring system used, but commonly include scores related to location, accuracy and organization. Each condition of the ROCF takes 10 min to complete and the overall time of completion is about 30 min. 相似文献
37.
38.
Seol B Gomercić MD Naglić T Gomercić T Galov A Gomercić H 《Journal of wildlife diseases》2006,42(3):709-711
Bacteriologic examination of an abscess found between blubber and musculature of a striped dolphin (Stenella coeruleoalba) found dead on 30 March 2002 in the Adriatic Sea, Croatia, was performed and an aerotolerant, nontoxogenic Clostridium tertium was isolated in pure culture. National Collections of Industrial Food and Marine Bacteria (NCIMB Ltd., Aberdeen, Scotland, UK) confirmed the results. Sequencing results showed it to be C. tertium with 100% similarity. The strain was named Clostridium tertium strain Zagreb, acceded to the culture collection and assigned the accession number NCIMB 13970. This is the first record of C. tertium in marine mammals. 相似文献
39.
40.
Shin JN Park SY Cha JH Park JY Lee BR Jung SA Lee ST Yun CW Seol DW Kim TH 《Experimental cell research》2006,312(19):3892-3898
TRAIL has been suggested to induce the cell death in various tumor cells but not in normal cells; however, several studies have provided the evidence that TRAIL can induce the cell death in some normal cells including human normal hepatocytes, suggesting that TRAIL may show hepatic toxicity in human. In this study, we designed a pro-form of TRAIL (sTRAIL:IL-18) in that soluble TRAIL (sTRAIL) is fused to IL-18, and a matrix metalloproteinases (MMPs) cleavage site is introduced at the connecting site. We showed that sTRAIL:IL-18 has significantly diminished the killing activity in HeLa cells but regains the activity by releasing the free sTRAIL through MMP-2-mediated cleavage. In addition, the killing activity of sTRAIL:IL-18 was significantly increased in HeLa cells when active MMP-2 was produced by TNF-alpha. Taken together, the data suggested that the sTRAIL:IL-18 can be reactivated at the specialized areas where MMPs are pathologically produced. 相似文献