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11.
L A Drachev A A Jasaitis H Mikelsaar I B Nemecek A Y Semenov E G Semenova I I Severina V P Skulachev 《The Journal of biological chemistry》1976,251(22):7077-7082
1. Generation of a transmembrane electric potential difference by oligomycin-sensitive ATPase complex, incorporated into spherical or planar phospholipid membrane, has been demonstrated. To this end, penetrating anion probe and direct voltmeter measurement of electric potential across phospholipid membrane were used. It was found that ATP-induced electric response is sensitive to oligomycin and protonophorous uncouplers. 2. The effect of variations in the phospholipid component of proteoliposomes on the electric generation was studied. It was revealed that the usage of mitochondrial phospholipids and phosphatidylethanolamine allows the highest values of membrane potential to be obtained in the case of ATPase proteoliposomes. In the case of cytochrome oxidase and bacteriorhodopsin proteoliposomes, phosphatidylserine was also shown to be quite suitable. Phosphatidylcholine was absolutely ineffective in all cases. 3. In proteoliposomes, containing both ATPase and bacteriorhodopsin, ATP and light induced generation of the electric field of the same direction. 4. In ATPase + cytochrome oxidase proteoliposomes, ATP hydrolysis and ascorbate oxidation was found to support electric generation of the same direction if cytochrome c was inside vesicles. Oxidation via external cytochrome c resulted in formation of electric field of the direction, opposite to that induced by ATP hydrolysis. 5. The data obtained in experiments with proteoliposomes of different types are discussed. The conclusion is made that conversion of energy of different resources into electric form is a common feature of membraneous energy transducers, which is in agreement with the Mitchellian principle of cellular energetics. 相似文献
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Doklady Biological Sciences - This paper presents an updated mathematical model of the evolutionary dynamics of the sexual conflict (“battle of the sexes”) and its simulated... 相似文献
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Stroganov A. N. Bleil M. Oeberst R. Winkler H. Semenova A. V. 《Russian Journal of Genetics》2013,49(9):937-944
Russian Journal of Genetics - Using the AGP*, PGI-1*, PGI-2*, LDH*, IDH*, and PGM* allozyme markers, the differentiation of cod groups during the spawning period in Baltic Sea was evaluated. It was... 相似文献
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Kuzishschin K. V. Semenova A. V. Gruzdeva M. A. Pavlov D. S. 《Journal of Ichthyology》2020,60(6):839-857
Journal of Ichthyology - The diversity of life strategy, population structure, size, age and sex composition, growth and variability of 11 microsatellite loci in mykiss (rainbow trout) Parasalmo... 相似文献
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Natalya Semenova Irina Madaeva Tatyana Bairova Sergey Kolesnikov Lubov Kolesnikova 《Chronobiology international》2013,30(10):1399-1408
ABSTRACTA comparative analysis of lipid peroxidation processes and antioxidant defense system in Caucasian menopausal women with/without insomnia depending on the genotype of Clock 3111T/C gene polymorphism was performed. Two hundred and fourteen Caucasian menopausal women divided into control (without insomnia) and main group (with insomnia) were examined. Lipid peroxidation (conjugated dienes, thiobarbituric acid reactants) and antioxidant defense system parameters (?-tocopherol, retinol, reduced and oxidized glutathione, glutathione S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase) were determined by spectrofluorophotometer and immunoenzymometric methods. Patients with insomnia carriers of the TT-genotype had a significantly higher thiobarbituric acid reactants level and glutathione peroxidase activity as compared to group with insomnia carriers of the minor 3111C-allele (p < .05). A comparative analysis of the parameters in the women of the main and control groups showed higher conjugated dienes, thiobarbituric acid reactants levels and lower retinol, reduced glutathione levels, glutathione reductase activity in women with insomnia carriers of the TT-genotype (p < .05). The carriers of the minor allele with insomnia had a higher conjugated dienes levels and lower glutathione peroxidase activity as compared to control (p < .05). Thus, lipid peroxidation and antioxidant system parameters in Caucasian menopausal women with insomnia depend on the Clock 3111T/C gene polymorphism. 相似文献
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A. P. Smolov A. M. Butanaev G. A. Semenova G. N. Shirshikova 《Cell and Tissue Biology》2013,7(6):578-585
This paper presents a comparative analysis of obtained experimental data on the effect of exogenous ammonium on the protein and chlorophyll content, on the number of ribosomal structures, and on expression of the ribosomal genes encoding protein of small subunit rpS6 and 18S rRNA in cells of the soybean callus culture Glycine max and of unicellular green alga Chlamydomonas reinhardtii. It has been shown that, under the effect of exogenous ammonium. an increase of the number ribosomal structures in cells of the soybean callus, as well as in the alga cells, is not caused by an increase in the activity of the corresponding ribosomal genes. Possible mechanisms of action of ammonium on the content both of ribosomes in cells and of their protein and chlorophyll are discussed. 相似文献
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S. P. Makarenko V. N. Shmakov L. V. Dudareva A. V. Stolbikova N. V. Semenova I. N. Tret’yakova Yu. M. Konstantinov 《Russian Journal of Plant Physiology》2016,63(2):252-258
Fatty acid (FA) composition of total lipids in embryogenic and nonembryogenic calli of Siberian larch (Larix sibirica Ledeb.) was investigated by the method of GC-MS. We detected a high content of oleic acid in total lipids of embryogenic cell culture (32–56% by weight of total FA), which apparently depends on a high activity of acyl carrier protein (stearoyl-ACP-Δ9-desat-urase). At the same time, activity of Δ12-desaturase in the cells of embryogenic calli was considerably (2–3 times) lower than in nonembryogenic calli. We discuss a possibility that concentration of FA (oleic and linoleic acids) may be used as a marker of embryogenic potential when promising cell lines of Siberian larch are screened in the stage of early embryogenesis. 相似文献