全文获取类型
收费全文 | 237篇 |
免费 | 20篇 |
出版年
2021年 | 2篇 |
2020年 | 2篇 |
2018年 | 5篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 16篇 |
2014年 | 12篇 |
2013年 | 6篇 |
2012年 | 8篇 |
2011年 | 11篇 |
2010年 | 7篇 |
2009年 | 10篇 |
2008年 | 4篇 |
2007年 | 10篇 |
2006年 | 4篇 |
2005年 | 4篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 6篇 |
2001年 | 6篇 |
1999年 | 4篇 |
1998年 | 11篇 |
1997年 | 10篇 |
1996年 | 9篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 6篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1985年 | 8篇 |
1984年 | 3篇 |
1983年 | 6篇 |
1982年 | 5篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 10篇 |
1976年 | 7篇 |
1975年 | 6篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1968年 | 1篇 |
1959年 | 1篇 |
排序方式: 共有257条查询结果,搜索用时 140 毫秒
41.
42.
43.
JC. Brisset F. Gazeau C. Corot N. Nighoghossian Y. Berthezène E. Canet-Soulas M. Wiart 《IRBM》2018,39(2):93-102
Background
Inflammation is a share process in atherosclerosis and stroke and is thought to be a key player in the evolution of these diseases. Ten years ago, inflammation imaging with magnetic resonance imaging (MRI) was considered very promising for both pre-clinical and clinical studies of atherosclerosis and stroke.Contribution
We report here contributions to the field of inflammation imaging with USPIO-enhanced MRI. The goal was to investigate the life cycle of USPIOs in the body, and how the MRI signal has been impacted during their bio-interactions and bioprocessing. Those mechanisms were applied to pre-clinical longitudinal studies of inflammation in atherosclerosis and at the acute stage of ischemic stroke thus allowing the monitoring of treatment effects.Conclusion
This review presents the contribution of the collaborative research project under the “TecSan” grant from the French Research Agency (ANR) as well as pre-clinical and clinical perspectives of USPIO's inflammation MRI in atherosclerosis and stroke. 相似文献44.
Nonneutral evolution of tandem repeats in the mitochondrial DNA control region of lagomorphs 总被引:7,自引:0,他引:7
Casane D; Dennebouy N; de Rochambeau H; Mounolou JC; Monnerot M 《Molecular biology and evolution》1997,14(8):779-789
The mitochondrial DNA of the European rabbit (Oryctolagus cuniculus)
contains a tandem array of 153-bp repeats in the vicinity of the
replication origin of the H-stand. Variation among molecules in the number
of these repeats results in inter- and intraindividual length polymorphism
(heteroplasmy). Generally, in an individual, one predominant molecular type
is observed, the others representing a low percentage of the mtDNA content.
At the tissue level, we observe a particular distribution of this
polymorphism in the gonads compared with liver, kidneys, or brain, implying
a relationship between the differentiation status of the cells and the
types of new mtDNA molecules which appear and accumulate during lifetime.
Similar tandem repeats were also found in the mtDNA noncoding region of
European hares (Lepus europaeus), a cottontail (Sylvilagus floridanus), and
a pika (Ochotona rufescens). The lengths and the sequences of these units
evolve rapidly and in a concerted way, but the number of repeats is
maintained in a narrow range, and an internal 20-bp segment is highly
conserved. Constraints restrict the evolution of the primary sequence of
these repeated units, the number of which is probably controlled by a
stabilizing selection.
相似文献
45.
Distinguishing morphologically cryptic taxa, by definition, requires genetic data such as DNA sequences. However, DNA sequences may not be obtained easily for taxa from remote sites. Here we provide the details of a high-resolution melt-curve-based method using taxon-specific primers that can distinguish two taxa of Adélie penguins, and that will be usable in Antarctica when combined with some of the newly developed field-deployable thermal cyclers. We suggest that the wider adoption of field-deployable polymerase-chain-reaction-based techniques will enable faster assignation of haplotype to individuals in situ, and so allow the targeting of observations and sample collection to specimens relevant to the research question. Targeting individuals will also reduce the need to repeatedly handle animals and reduce the time and travel required to complete field work. 相似文献
46.
47.
Rupert CM Jones Maria Dickson-Spillmann Martin JC Mather Dawn Marks Bryanie S Shackell 《Respiratory research》2008,9(1):62
Background
Guidelines on COPD diagnosis and management encourage primary care physicians to detect the disease at an early stage and to treat patients according to their condition and needs. Problems in guideline implementation include difficulties in diagnosis, using spirometry and the disputed role of reversibility testing. These lead to inaccurate diagnostic registers and inadequacy of administered treatments. This study represents an audit of COPD diagnosis and management in primary care practices in Devon.Methods
Six hundred and thirty two patients on COPD registers in primary care practices were seen by a visiting Respiratory Specialist Nurse. Diagnoses were made according to the NICE guidelines. Reversibility testing was carried out either routinely or based on clinical indication in two sub-samples. Dyspnoea was assessed. Data were entered into a novel IT-based software which computed guideline-based treatment recommendations. Current and recommended treatments were compared.Results
Five hundred and eighty patients had spirometry. Diagnoses of COPD were confirmed in 422 patients (73%). Thirty nine patients were identified as asthma only, 94 had normal spirometry, 23 were restrictive and 2 had a cardiac disorder. Reversibility testing changed diagnosis of 11% of patients with airflow obstruction, and severity grading in 18%. Three quarters of patients with COPD had been offered practical help with smoking cessation. Short and long-acting anticholinergics and long acting beta-2 agonists had been under-prescribed; in 15–18% of patients they were indicated but not received. Inhaled steroids had been over-prescribed (recommended in 17%; taken by 60%), whereas only 4% of patients with a chronic productive cough were receiving mucolytics. Pulmonary rehabilitation was not available in some areas and was under-used in other areas.Conclusion
Diagnostic registers of COPD in primary care contain mistakes leading to inaccurate prevalence estimates and inappropriate treatment decisions. Use of pre-bronchodilator readings for diagnosis overestimates the prevalence and severity in a significant minority, thus post bronchodilator readings should be used. Management of stable COPD does often not correspond to guidelines. The IT system used in this study has the potential to improve diagnosis and management of COPD in primary care. 相似文献48.
PJS Amaral LFM Finotelo EHC De Oliveira A Pissinatti CY Nagamachi JC Pieczarka 《BMC evolutionary biology》2008,8(1):169
Background
Chromosomal painting, using whole chromosome probes from humans and Saguinus oedipus, was used to establish karyotypic divergence among species of the genus Cebus, including C. olivaceus, C. albifrons, C. apella robustus and C. apella paraguayanus. Cytogenetic studies suggested that the species of this genus have conservative karyotypes, with diploid numbers ranging from 2n = 52 to 2n = 54. 相似文献49.
Maria João Fernandes Martins Francesc Mezquita Olivier Schmit Juan Rueda Giampaolo Rossetti Tadeusz Namiotko 《Hydrobiologia》2009,636(1):219-232
Eucypris virens is a freshwater ostracod in which both sexual reproduction and parthenogenesis occur. Sympatric coexistence of both reproductive
modes is known in zones of overlap. This renders the species a potentially valuable model organism to study the ‘queen of
evolutionary problems’, i.e. why sex is so successful despite its costs (paradox of sex). In order to maximally exploit this
potential, a broad knowledge of the species’ ecology is essential, including an understanding of its life history and population
dynamics. Here, the phenology of the species was followed in three temporary ponds through monthly (Spain) or fortnightly
(Poland) samplings, throughout an inundation period. This study confirms the wide ecological tolerances of E. virens. Although the species is generally assumed to be univoltine, two hatching periods were observed in the Spanish sites. Biotic
interactions, especially predation, appear to be the important determinants of population dynamics in long-hydroperiod sites.
Abiotic conditions may influence population dynamics through their impact on egg hatching. In the site with male presence,
the initially female-biased sex ratio evolved towards a balanced sex ratio through the season. No consistent differences in
limb morphology were observed between females originating from the three study sites. On the other hand, valve size of adult
females varied among sites, possibly influenced by local environmental conditions (mainly salinity and pH) as well as the
expected genetic diversity. 相似文献
50.
Sylvie Tordjman George M. Anderson Michel Botbol Sylvie Brailly-Tabard Fernando Perez-Diaz Rozenn Graignic Michèle Carlier Gérard Schmit Anne-Catherine Rolland Olivier Bonnot Séverine Trabado Pierre Roubertoux Guillaume Bronsard 《PloS one》2009,4(8)