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461.
The insect-hormone ecdysterone causes a de novo synthesis of RNA in the fat body cells of blowfly larvae. Hybridization of the RNA induced by the hormone with 3H-Poly(U) demonstrates that it contains poly(A) sequences and therefore is probably informational. DNA-RNA-hybridization experiments further show that qualitatively new mRNA species are synthesized under the influence of ecdysterone. The inducibility of RNA synthesis is strongly correlated to specific developmental stages. 相似文献
462.
Molecular dissection of photosystem I in higher plants: topology, structure and function 总被引:2,自引:0,他引:2
Poul Erik Jensen Anna Haldrup Lisa Rosgaard Henrik Vibe Scheller 《Physiologia plantarum》2003,119(3):313-321
Photosystem I catalyses the light driven electron transfer from plastocyanin/cytochrome c 6 on the lumenal side of the thylakoid membrane to ferredoxin/flavodoxin at the stromal side. Photosystem I of higher plants consists of 18 different protein subunits. Fourteen of these make up the chlorophyll a -containing core, which also contains the cofactors involved in the electron transfer reactions, and four make up the peripheral chlorophyll a / b -containing antenna. Arabidopsis plants devoid of the nuclear-encoded photosystem I subunits have been obtained either by different suppression techniques or by insertional knock-out of the genes. This has allowed a detailed analysis of the role and function of the individual subunits. This review is focused on recent developments in the role of the individual subunit in the structure and function of photosystem I of higher plants. 相似文献
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Photosystem I Is an Early Target of Photoinhibition in Barley
Illuminated at Chilling Temperatures 总被引:11,自引:0,他引:11 下载免费PDF全文
Staffan Erling Tjus Birger Lindberg M?ller Henrik Vibe Scheller 《Plant physiology》1998,116(2):755-764
Light-induced damage to photosystem I (PSI) was studied during low-light illumination of barley (Hordeum vulgare L.) at chilling temperatures. A 4-h illumination period induced a significant inactivation of PSI electron transport activity. Flash-induced P700 absorption decay measurements revealed progressive damage to (a) the iron-sulfur clusters FA and FB, (b) the iron-sulfur clusters FA, FB, and FX, and (c) the phylloquinone A1 and the chlorophyll A0 or P700 of the PSI electron acceptor chain. Light-induced PSI damage was also evidenced by partial degradation of the PSI-A and PSI-B proteins and was correlated with the appearance of smaller proteins. Aggravated photodamage was observed upon illumination of barley leaves infiltrated with KCN, which inhibits Cu,Zn-superoxide dismutase and ascorbate peroxidase. This indicates that the photodamage of PSI in barley observed during low-light illumination at chilling temperatures arises because the defense against active oxygen species by active oxygen-scavenging enzymes is insufficient at these specific conditions. The data obtained demonstrate that photoinhibition of PSI at chilling temperatures is an important phenomenon in a cold-tolerant plant species. 相似文献