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排序方式: 共有293条查询结果,搜索用时 156 毫秒
71.
Takayuki Imai Keiichi Tamai Sayuri Oizumi Kyoko Oyama Kazunori Yamaguchi Ikuro Sato Kennichi Satoh Kazuto Matsuura Shigeru Saijo Kazuo Sugamura Nobuyuki Tanaka 《PloS one》2013,8(4)
Cancer stem cells contribute to the malignant phenotypes of a variety of cancers, but markers to identify human hypopharyngeal cancer (HPC) stem cells remain poorly understood. Here, we report that the CD271+ population sorted from xenotransplanted HPCs possesses an enhanced tumor-initiating capability in immunodeficient mice. Tumors generated from the CD271+ cells contained both CD271+ and CD271− cells, indicating that the population could undergo differentiation. Immunohistological analyses of the tumors revealed that the CD271+ cells localized to a perivascular niche near CD34+ vasculature, to invasive fronts, and to the basal layer. In accordance with these characteristics, a stemness marker, Nanog, and matrix metalloproteinases (MMPs), which are implicated in cancer invasion, were significantly up-regulated in the CD271+ compared to the CD271− cell population. Furthermore, using primary HPC specimens, we demonstrated that high CD271 expression was correlated with a poor prognosis for patients. Taken together, our findings indicate that CD271 is a novel marker for HPC stem-like cells and for HPC prognosis. 相似文献
72.
Satoshi Yamamoto Yuki Ooshima Mitsugu Nakata Takashi Yano Kunio Matsuoka Sayuri Watanabe Ryouta Maeda Hideki Takahashi Michiyasu Takeyama Yoshio Matsumoto Tadatoshi Hashimoto 《Transgenic research》2013,22(3):537-547
Gene-targeting technology using mouse embryonic stem (ES) cells has become the “gold standard” for analyzing gene functions and producing disease models. Recently, genetically modified mice with multiple mutations have increasingly been produced to study the interaction between proteins and polygenic diseases. However, introduction of an additional mutation into mice already harboring several mutations by conventional natural crossbreeding is an extremely time- and labor-intensive process. Moreover, to do so in mice with a complex genetic background, several years may be required if the genetic background is to be retained. Establishing ES cells from multiple-mutant mice, or disease-model mice with a complex genetic background, would offer a possible solution. Here, we report the establishment and characterization of novel ES cell lines from a mouse model of Alzheimer’s disease (3xTg-AD mouse, Oddo et al. in Neuron 39:409–421, 2003) harboring 3 mutated genes (APPswe, TauP301L, and PS1M146V) and a complex genetic background. Thirty blastocysts were cultured and 15 stable ES cell lines (male: 11; female: 4) obtained. By injecting these ES cells into diploid or tetraploid blastocysts, we generated germline-competent chimeras. Subsequently, we confirmed that F1 mice derived from these animals showed similar biochemical and behavioral characteristics to the original 3xTg-AD mice. Furthermore, we introduced a gene-targeting vector into the ES cells and successfully obtained gene-targeted ES cells, which were then used to generate knockout mice for the targeted gene. These results suggest that the present methodology is effective for introducing an additional mutation into mice already harboring multiple mutated genes and/or a complex genetic background. 相似文献
73.
Satoshi Kusuda Yuka Kakizoe Koji Kanda Tomoko Sengoku Yohei Fukumoto Itsuki Adachi Yoko Watanabe Osamu Doi 《Zoo biology》2011,30(3):285-295
This study aimed to validate the measurements of body temperature and fecal progesterone concentrations as minimally invasive techniques for assessing ovarian cycle in a single sexually mature female killer whale. Rectal temperature data, fecal and blood samples were collected in the dorsal position using routine husbandry training on a voluntary basis. The correlations between rectal temperature and plasma progesterone concentration and between fecal and plasma progesterone concentrations were investigated. Fecal progesterone metabolites were identified by a combination of high‐performance liquid chromatography and enzyme immunoassay. Plasma progesterone concentrations (range: 0.2–18.6 ng/ml) and rectal temperature (range: 35.3–35.9°C) changed cyclically, and cycle lengths were an average (±SD) of 44.9±4.0 days (nine cycles) and 44.6±5.9 days (nine cycles), respectively. Rectal temperature positively correlated with the plasma progesterone concentrations (r=0.641, P<0.01). There was a visual trend for fecal progesterone profiles to be similar to circulating plasma progesterone profiles. Fecal immunoreactive progestagen analysis resulted in a marked immunoreactive peak of progesterone. The data from the single killer whale indicate that the measurement of rectal temperature is suitable for minimally invasive assessment of the estrous cycle and monitoring the fecal progesterone concentration is useful to assess ovarian luteal activity. Zoo Biol 30:285–295, 2011. © 2010 Wiley‐Liss, Inc. 相似文献
74.
Ishii A Nonaka T Taniguchi S Saito T Arai T Mann D Iwatsubo T Hisanaga S Goedert M Hasegawa M 《FEBS letters》2007,581(24):4711-4717
In Lewy body diseases and multiple system atrophy, α-synuclein is hyperphosphorylated at Ser129, suggesting a role in pathogenesis. Here, we report purification of the protein kinase in rat brain that phosphorylates Ser129 and its identification as casein kinase-2 (CK2). We show that most of the activity can be inhibited by heparin, an inhibitor of CK2. Phosphorylated Ser129 was detected in primary cultured neurons and inhibited by CK2 inhibitors. In some cases of Lewy body disease, CK2-like immunoreactivity was recovered in the sarkosyl-insoluble fraction, which was enriched in phosphorylated α-synuclein. Taken together, these findings suggest that CK2 may be involved in the hyperphosphorylation of α-synuclein in α-synucleinopathies. 相似文献
75.
Carreira RS Miyamoto S Di Mascio P Gonçalves LM Monteiro P Providência LA Kowaltowski AJ 《Journal of bioenergetics and biomembranes》2007,39(4):313-320
This study tests the hypothesis that ischemic preconditioning (IP) changes fatty acid (FA)-dependent uncoupling between mitochondrial
respiration and oxidative phosphorylation. We found that IP does not alter mitochondrial membrane integrity or FA levels,
but enhances membrane potential decreases when FA are present, in an ATP-sensitive manner. FA hydroperoxides had equal effects
in control and preconditioned mitochondria, and GTP did not abrogate the IP effect, suggesting uncoupling proteins were not
involved. Conversely, thiol reductants and atractyloside, which inhibits the adenine nucleotide translocator, eliminated the
differences in responses to FA. Together, our results suggest that IP leads to thiol oxidation and activation of the adenine
nucleotide translocator, resulting in enhanced FA transport and mild mitochondrial uncoupling. 相似文献
76.
Mesenchymal progenitor cells in adult human dental pulp and their ability to form bone when transplanted into immunocompromised mice 总被引:3,自引:0,他引:3
Otaki S Ueshima S Shiraishi K Sugiyama K Hamada S Yorimoto M Matsuo O 《Cell biology international》2007,31(10):1191-1197
The technique of tissue engineering is developing for the restoration of lost tissues. This new technique requires cells that fabricate tissue. Mesenchymal stem cells in bone marrow have been used as the cell source for this technique; however, dental pulp cells have recently been shown to possess stem-cell-like properties. We earlier demonstrated that dental pulp cells proliferate and produce an extracellular matrix that subsequently becomes mineralized in vitro. We now report that such dental pulp cells (first to eighth passage) produced bone instead of dentin when those cells were implanted into subcutaneous sites in immunocompromised mice with HA/TCP powder as their carrier. This evidence shows that dental pulp cells are the common progenitors of odontoblasts and osteoblasts, or dental pulp cells are mesenchymal stem cells themselves. It is expected that dental pulp cells can be a useful candidate cell source for tissue engineering, and contain the potential of new therapeutic approaches for the restoration of damaged or diseased tissue. 相似文献
77.
David Cícera Edna Barbosa Lucas Aline Maria Brito Cunha Pedro Lourenzo Oliveira Viana Yuana Ivia Ponte Yoshinaga Marcos Yukio Miyamoto Sayuri Filho Adriano Brito Chaves Varela Anna Lídia Nunes Kowaltowski Alicia Juliana Facundo Heberty Tarso 《Journal of physiology and biochemistry》2022,78(1):283-294
Journal of Physiology and Biochemistry - Typically, healthy cardiac tissue utilizes more fat than any other organ. Cardiac hypertrophy induces a metabolic shift leading to a preferential... 相似文献
78.
Odagiri Naoshi Matsubara Tsutomu Higuchi Moe Takada Sayuri Urushima Hayato Sato-Matsubara Misako Teranishi Yuga Yoshizato Katsutoshi Kawada Norifumi Ikeda Kazuo 《Molecular and cellular biochemistry》2019,455(1-2):7-19
Molecular and Cellular Biochemistry - Senescent hepatic stellate cells (senescent HSCs) are found in patients with liver cirrhosis and have been thought to be involved in the development of... 相似文献
79.
80.
Takushi Nomura Hiroyuki Yamamoto Masako Nishizawa Trang Thi Thu Hau Shigeyoshi Harada Hiroshi Ishii Sayuri Seki Midori Nakamura-Hoshi Midori Okazaki Sachie Daigen Ai Kawana-Tachikawa Noriyo Nagata Naoko Iwata-Yoshikawa Nozomi Shiwa Shun Iida Harutaka Katano Tadaki Suzuki Eun-Sil Park Ken Maeda Yuriko Suzaki Yasushi Ami Tetsuro Matano 《PLoS pathogens》2021,17(7)
SARS-CoV-2 infection presents clinical manifestations ranging from asymptomatic to fatal respiratory failure. Despite the induction of functional SARS-CoV-2-specific CD8+ T-cell responses in convalescent individuals, the role of virus-specific CD8+ T-cell responses in the control of SARS-CoV-2 replication remains unknown. In the present study, we show that subacute SARS-CoV-2 replication can be controlled in the absence of CD8+ T cells in cynomolgus macaques. Eight macaques were intranasally inoculated with 105 or 106 TCID50 of SARS-CoV-2, and three of the eight macaques were treated with a monoclonal anti-CD8 antibody on days 5 and 7 post-infection. In these three macaques, CD8+ T cells were undetectable on day 7 and thereafter, while virus-specific CD8+ T-cell responses were induced in the remaining five untreated animals. Viral RNA was detected in nasopharyngeal swabs for 10–17 days post-infection in all macaques, and the kinetics of viral RNA levels in pharyngeal swabs and plasma neutralizing antibody titers were comparable between the anti-CD8 antibody treated and untreated animals. SARS-CoV-2 RNA was detected in the pharyngeal mucosa and/or retropharyngeal lymph node obtained at necropsy on day 21 in two of the untreated group but undetectable in all macaques treated with anti-CD8 antibody. CD8+ T-cell responses may contribute to viral control in SARS-CoV-2 infection, but our results indicate possible containment of subacute viral replication in the absence of CD8+ T cells, implying that CD8+ T-cell dysfunction may not solely lead to viral control failure. 相似文献