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Ethanol from fermentation is usually estimated by gas chromatography after centrifuging or distilling the broth. In this paper a more efficient and rapid method is described in which ethanol is extracted by an organic solvent such as n-butanol and the extract is analysed by gas chromatography. The distribution factor determined has a value close to unity and is dependent on ethanol concentration, but independent of sugar concentration. 相似文献
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Ajay Kumar N. Usha P. D. Sawant R. M. Tripathi Sanu S. Raj Manish Mishra 《人类与生态风险评估》2011,17(2):381-393
Traces of uranium were measured by laser fluorimeter in 235 subsurface water samples collected from four districts of Punjab state in India. The concentration of U in water samples ranged between <2–644 μg/L with a mean value of 73.1 μg/L. The radiological risk was observed to be in the range of 5.55 × 10?6–1.78 × 10?3 with a mean value of 2.03 × 10?4, which is around 22% more than the maximum acceptable level (l.67 × 10?4) as per guidelines of India's Atomic Energy Regulatory Board. The mean of chemical toxicity risk, expressed as life time average daily dose (LADD) was worked out to be 5.56 μg/kg/day with a range of 0.15–48 μg/kg/day by considering a bodyweight of 51.5 ± 8.5 kg, water ingestion rate of 4.05 L/d, and life expectancy of 63.7 yrs for an adult Indian reference man and compared with the reference dose (4.53 μg/kg/day). The average exposure level of U was comparatively high and the chemical toxicity was expected to be more. The mean of hazard quotient (LADD/ RfD) for all four districts was found to be greater than 1, indicating that groundwater may not be suitable for consumption from a chemical toxicity point of view. 相似文献
166.
P. A. Parment C. Svanborg-Ede'n M. J. Chaknis A. D. Sawant L. Hagberg L. A. Wilson Dr. D. G. Ahearn 《Current microbiology》1992,25(2):113-118
The capacity of 59 isolates of Serratia marcescens, obtained from urinary tract infections, wounds, and contact lenses or their paraphernalia, to agglutinate erythrocytes from different animal species was tested. Three main patterns were found: mannose-sensitive agglutination of guinea-pig, fowl or horse erythrocyte; mannose-resistant agglutination of chicken or pigeon erythrocytes alone or in combination with mannose-sensitive agglutination; and no agglutination. Hemagglutination capacity was associated with isolates from urinary tract infection, but not with isolates associated with contact lenses. Adherence to human urinary tract epithelium did not correlate with the hemagglutination patterns nor with the origin of the isolates. Some strains of different hemagglutination pattern were selected for the study of hydrophobicity and adherence to contact lens polymers. Hydrophobicity, as determined by degree of partition in hexadecane and water (BATH-values), correlated neither with degree of adherence to contact lens polymers nor with the hemagglutination pattern. For a representative strain there was an excellent correlation (r2 = 0.98) between adherence and the water content (hydrophobicity) of the lens polymers. These results suggest that, as with tissues, other factors interact with hydrophobicity in causing adherence to plastics. 相似文献
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V. Hegde M. Vijayan S. Kumar Md Akheruzzaman N. Sawant N.V. Dhurandhar P.H. Reddy 《生物化学与生物物理学报:疾病的分子基础》2019,1865(11):165531
Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder worldwide. While the causes of AD are unclear, several risk factors have been identified, including impaired glycemic control, which significantly increases the risk of cognitive decline and AD. In vitro and in vivo studies show that human adenovirus 36 (Ad36) improves glycemic control by increasing cellular glucose uptake in cells, experimental animal models and in humans who are naturally exposed to the virus. This study, tested improvement in glycemic control by Ad36 and delay in onset of cognitive decline in APPswe transgenic mice (Tg2576 line), a model of genetic predisposition to impaired glycemic control and AD. Three-month old APPswe mice were divided into Ad36 infected (Ad36) or mock infected (control) groups and baseline glycemic control measured by glucose tolerance test (GTT) prior to infection. Changes in glycemic control were determined 10- and 24-week post infection. Serum insulin was also measured during GTT. Cognition was determined by Y-maze test, while motor coordination and skill acquisition by rotarod test. Glycemic control as determined by GTT showed less deterioration in Ad36 infected mice over time, accompanied by a significant attenuation of cognitive decline. Analysis of brain tissue lysate showed significantly reduced levels of amyloid beta 42 in Ad36 mice relative to control mice. Golgi-Cox staining analysis also revealed reduced dendritic spines and synaptic gene expression in control mice compared to Ad36 infected mice. This proof of concept study shows that in a mouse model of AD, Ad36 improves glycemic control and ameliorates cognitive decline. 相似文献
169.
D. L. Price A. D. Sawant D. G. Ahearn 《Journal of industrial microbiology & biotechnology》1991,8(2):83-89
Summary Growth and survival ofStaphylococcus aureus, Pseudomonas aeruginosa., Klebsiella pneumoniae andAspergillus niger were reduced by a low-solubility polysubstituted.-quaternary amine complex (InterseptR) processed into the matrices of ethylene vinyl acetate, polystyrene and polyethylene., Recoveries of challenge microorganisms from agar film overlays and determination of the effects of the complex on radiolabelled-leucine transport by adhered cells and bacterial biofilms were more suitable for assessing inhibitory efficacies than standard agar diffusion or serial dilution procedures. 相似文献
170.
V. M. Hanchinal S. A. Survase S. K. Sawant U. S. Annapure 《Plant Cell, Tissue and Organ Culture》2008,93(2):123-132
Plants are a valuable source of a vast array of chemical compounds including fragrances, flavours, food additives, colours,
natural sweeteners, industrial feedstocks, anti-microbials and pharmaceuticals. The present study reports on application of
Response Surface Methodology (RSM) in media optimization for suspension culture for the production of β-carotene. Growth kinetics
of carrot cells in suspension culture has been carried out to understand the relationship between growth and β-carotene formation.
The maximum production of β-carotene obtained using the optimized medium was 13.614 μg/g dry weight cell mass. The μ (specific growth rate) and t
d
(doubling time) were found to be higher for 20 g DW/l inoculum size. 相似文献