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41.
Ashirbad Pradhan Anita Anasuya Madan Mohan Pradhan Kavitha AK Priyanka Kar Krushna Chandra Sahoo Pinaki Panigrahi Ambarish Dutta 《PloS one》2016,11(2)
Background
Although Odisha is the largest contributor to the malaria burden in India, no systematic study has examined its malaria trends. Hence, the spatio-temporal trends in malaria in Odisha were assessed against the backdrop of the various anti-malaria strategies implemented in the state.Methods
Using the district-wise malaria incidence and blood examination data (2003–2013) from the National Vector Borne Disease Control Program, blood examination-adjusted time-trends in malaria incidence were estimated and predicted for 2003–2013 and 2014–2016, respectively. An interrupted time series analysis using segmented regression was conducted to compare the disease trends between the pre (2003–2007) and post-intensification (2009–2013) periods. Key-informant interviews of state stakeholders were used to collect the information on the various anti-malaria strategies adopted in the state.Results
The state annual malaria incidence declined from 10.82/1000 to 5.28/1000 during 2003–2013 (adjusted annual decline: -0.54/1000, 95% CI: -0.78 to -0.30). However, the annual blood examination rate remained almost unchanged from 11.25% to 11.77%. The keyinformants revealed that intensification of anti-malaria activities in 2008 led to a more rapid decline in malaria incidence during 2009–2013 as compared to that in 2003–2007 [adjusted decline: -0.83 (-1.30 to -0.37) and -0.27 (-0.41 to -0.13), respectively]. There was a significant difference in the two temporal slopes, i.e., -0.054 (-0.10 to -0.002, p = 0.04) per 1000 population per month, between these two periods, indicating almost a 200% greater decline in the post-intensification period. Although, the seven southern high-burden districts registered the highest decline, they continued to remain in that zone, thereby, making the achievement of malaria elimination (incidence <1/1000) unlikely by 2017.Conclusion
The anti-malaria strategies in Odisha, especially their intensification since 2008, have helped improve its malaria situation in recent years. These successful measures need to be sustained and perhaps intensified further for eliminating malaria from Odisha. 相似文献42.
43.
Background
Emerging whitefly transmitted begomoviruses are major pathogens of vegetable and fibre crops throughout the world, particularly in tropical and sub-tropical regions. Mutation, pseudorecombination and recombination are driving forces for the emergence and evolution of new crop-infecting begomoviruses. Leaf curl disease of field grown radish plants was noticed in Varanasi and Pataudi region of northern India. We have identified and characterized two distinct monopartite begomoviruses and associated beta satellite DNA causing leaf curl disease of radish (Raphanus sativus) in India.Results
We demonstrate that RaLCD is caused by a complex of two Old World begomoviruses and their associated betasatellites. Radish leaf curl virus-Varanasi is identified as a new recombinant species, Radish leaf curl virus (RaLCV) sharing maximum nucleotide identity of 87.7% with Tomato leaf curl Bangladesh virus-[Bangladesh:2] (Accession number AF188481) while the virus causing radish leaf curl disease-Pataudi is an isolate of Croton yellow vein mosaic virus-[India] (CYVMV-IN) (Accession number AJ507777) sharing 95.8% nucleotide identity. Further, RDP analysis revealed that the RaLCV has a hybrid genome, a putative recombinant between Euphorbia leaf curl virus and Papaya leaf curl virus. Cloned DNA of either RaLCV or CYVMV induced mild leaf curl symptoms in radish plants. However, when these clones (RaLCV or CYVMV) were individually co-inoculated with their associated cloned DNA betasatellite, symptom severity and viral DNA levels were increased in radish plants and induced typical RaLCD symptoms. To further extend these studies, we carried out an investigation of the interaction of these radish-infecting begomoviruses and their associated satellite, with two tomato infecting begomoviruses (Tomato leaf curl Gujarat virus and Tomato leaf curl New Delhi virus). Both of the tomato-infecting begomoviruses showed a contrasting and differential interaction with DNA satellites, not only in the capacity to interact with these molecules but also in the modulation of symptom phenotypes by the satellites.Conclusion
This is the first report and experimental demonstration of Koch's postulate for begomoviruses associated with radish leaf curl disease. Further observations also provide direct evidence of lateral movement of weed infecting begomovirus in the cultivated crops and the present study also suggests that the exchange of betasatellites with other begomoviruses would create a new disease complex posing a serious threat to crop production. 相似文献44.
45.
Eveline Jacques Kathleen Hectors Yves Guisez Els Prinsen Marcel AK Jansen Jean-Pierre Verbelen Kris Vissenberg 《Plant signaling & behavior》2011,6(1):83-85
Upon chronic UV treatment pavement cell expansion in Arabidopsis leaves is reduced, implying alterations in symplastic and apoplastic properties of the epidermal cells. In this study, the effect of UV radiation on microtubule patterning is analysed, as microtubules are thought to serve as guiding rails for the cellulose synthase complexes depositing cellulose microfibrils. Together with hemicelluloses, these microfibrils are regarded as the load-bearing components of the cell wall. Leaves of transgenic plants with fluorescently tagged microtubules (GFP-TUA6) were as responsive to UV as wild type plants. Despite the UV-induced reduction in cell elongation, confocal microscopy revealed that cellular microtubule arrangements were seemingly not affected by the UV treatments. This indicates an unaltered deposition of cellulose microfibrils in the presence of UV radiation. Therefore, we surmise that the reduction in cell expansion in UV-treated leaves is most probably due to changes in cell wall loosening and/or turgor pressure.Key words: arabidopsis, cell expansion, GFP-TUA6, leaf development, microtubule cytoskeleton, UV radiationPhotosynthetic functions such as solar light capture and carbon fixation are highly evolved features of plant leaves. To fulfil these functions in an optimal way, leaf development needs to be tuned to environmental conditions. Leaves are continuously exposed and subjected to environmental influences, which serve as co-regulators of leaf and plant development.1 This ability of plants to adapt, secures the plant''s survival, even under non-optimal conditions. An example of a regulatory environmental parameter is solar light, indispensable for photosynthesis but potentially causing photoinhibition and/or UV-radiation stress. The highly energetic ultraviolet B (UV-B) rays of short wavelengths (280–315 nm) can both cause damage, as well as induce a range of specific metabolic and morphogenic plant responses. It was reported before that exposure to low dose UV radiation reduces Arabidopsis leaf size due to a decreased cell size.2 Expansion of leaf epidermal cells of Arabidopsis thaliana is the combined action of promotion and restriction of growth, resulting in the typical irregular sinuous pavement cells. It has been postulated that cellulose microfibrils are responsible for generating a force opposing isotropic expansion by creating neck regions in between outgrowing lobes.3 As the microtubule cytoskeleton is believed to serve as guiding rails for the cellulose synthase complexes (CESAs),4 the deposition of the cellulose fibrils is intimately linked to the cortical microtubule arrangement. We have studied the UV-effect on microtubule organisation in leaf epidermal cells whose expansion had decreased upon this UV radiation. Microtubules in the adaxial pavement cells of the fourth leaf were monitored on several successive days in a transgenic line containing GFP fused to tubulin A6.5 The chronic UV treatment was started on day 0 when the plants were 2 weeks old, using UV exposure conditions as described in reference 2. First the responsiveness of the GFP-TUA6 plants to UV radiation was evaluated. Similar to wild type (WT) plants,2 the GFP-TUA6 plants had smaller leaves following 8 days of UV treatment (t-test, p < 0.01) (Fig. 1). This was caused by a significant reduction in the generalized cell area average of all measured cells, irrespective of the location within the leaf (Fig. 1; t-test, p < 0.01). In more detail, the average cell area within the base, middle and top zones of the GFP-TUA6 leaf was systematically lower in UV-treated leaves from 8 days after the treatment started onwards (data not shown).Open in a separate windowFigure 1Effect of UV radiation on leaf and cell area after different days of UV radiation. Open asterisks indicate a statistically significant difference in leaf area between UV-treated and control plants, black asterisks indicate statistically significant difference in cell area (t-test, *p < 0.05, **p < 0.01, ***p < 0.001). Error bars indicate the standard error for five different leaves at all measured time-points and 600, 170 and 180 cells at day 0, 8 and 12 respectively.As GFP-TUA6 leaves were as responsive to UV radiation as wild type leaves, confocal microscopy was used to visualize the organisation of the cortical microtubules facing the outer periclinal wall of the adaxial epidermis. No clear difference in microtubule (re)organization could be detected during the development of pavement cells, and throughout the UV treatment period. As shown in Figure 2 at day 2, pavement cells with comparable areas are similarly shaped in control and UV-irradiated plants and contain similar microtubule arrangements (Fig. 2 and marked cells). This means that microtubule organization is not directly affected by the UV exposure and that shape development proceeds in an analoguous manner as under control conditions. This lack of alteration in the microtubule arrangement can be observed for cells at the leaf tip, which were already in the process of lobe formation at the start of the exposure period, as well as for cells at the leaf base. Under our growth conditions, and in the monitored leaf number 4, cell proliferation still took place in this part of the leaf and lobes only started to appear on the cell surface. As microtubules are linked to the deposition of cellulose microfibrils, it can be assumed that no alterations in cellulose deposition occur upon UV treatment either. We can therefore conclude that the process of lobe formation and microtubule patterning is not impeded and that only the extent of cell expansion is restricted upon UV exposure.Open in a separate windowFigure 2Microtubule pattern in control and UV-exposed leaves visualized using GFP-TUA6 and confocal microscopy. Both images are from cells at the mid zone of the fourth leaf at day 2. Microtubules are similarly arranged in equally shaped and sized cells of control and UV-exposed leaves. The marked cells show a pattern whereby the tubules are centred in the neck regions between two outgrowing lobes.According to the Lockhart equation,6 cell (wall) growth is modulated by wall biomechanics and turgor pressure. Concerning turgor pressure, no clear differences in this factor between UV-exposed and control plants of Lactuca sativa L.7 and Pisum sativum8 could be observed, reinforcing the idea that especially the modulation of cell wall properties is the main factor causing the observed UV-induced reduction in cell expansion. Some reports indicate differential expression of wall loosening enzymes like expansins or xyloglucan endotransglycosylase/hydrolases (XTHs),9,10 or cell wall strengthening enzymes as particular peroxidases7 after UV exposure. Another key event could involve UV-mediated changes in the phenylpropanoid pathway, which may cause changes in the lignin biosynthesis. As shown by the literature11–14 lignin may well be an important modulator of cell wall architecture in Arabidopsis and therefore alterations in lignin synthesis could form the basis for morphological modifications. Further research on the cell wall properties of UV-treated plants may resolve this uncertainty.As a general conclusion we can state that the patterning of microtubules is not altered, but that alterations in cell wall composition or arrangements are the most plausible candidates for the observed reduction in pavement cell expansion upon chronic UV treatment. 相似文献
46.
Satapathy SK Ochani M Dancho M Hudson LK Rosas-Ballina M Valdes-Ferrer SI Olofsson PS Harris YT Roth J Chavan S Tracey KJ Pavlov VA 《Molecular medicine (Cambridge, Mass.)》2011,17(7-8):599-606
Obesity, a serious and growing health threat, is associated with low-grade inflammation that plays a role in mediating its adverse consequences. Previously, we have discovered a role for neural cholinergic signaling in controlling inflammation, and demonstrated that the cholinergic agent galantamine suppresses excessive proinflammatory cytokine release. The main objective of this study was to examine the efficacy of galantamine, a clinically-approved drug, in alleviating obesity-related inflammation and associated complications. After 8 wks on a high-fat diet, C57BL/6J mice were treated with either galantamine (4 mg/kg, intraperitoneally [i.p.]) or saline for 4 wks in parallel with mice on a low-fat diet and treated with saline. Galantamine treatment of obese mice significantly reduced body weight, food intake, abdominal adiposity, plasma cytokine and adipokine levels, and significantly improved blood glucose, insulin resistance and hepatic steatosis. In addition, galantamine alleviated impaired insulin sensitivity and glucose intolerance significantly. These results indicate a previously unrecognized potential of galantamine in alleviating obesity, inflammation and other obesity-related complications in mice. These findings are of interest for studying the efficacy of this clinically-approved drug in the context of human obesity and metabolic syndrome. 相似文献
47.
Hanna?B?Kastein Vinoth?AK?Kumar Sripathi?Kandula Sabine?SchmidtEmail author 《Frontiers in zoology》2013,10(1):75
Introduction
Immediate responses towards emotional utterances in humans are determined by the acoustic structure and perceived relevance, i.e. salience, of the stimuli, and are controlled via a central feedback taking into account acoustic pre-experience. The present study explores whether the evaluation of stimulus salience in the acoustic communication of emotions is specifically human or has precursors in mammals. We created different pre-experiences by habituating bats (Megaderma lyra) to stimuli based on aggression, and response, calls from high or low intensity level agonistic interactions, respectively. Then we presented a test stimulus of opposite affect intensity of the same call type. We compared the modulation of response behaviour by affect intensity between the reciprocal experiments.Results
For aggression call stimuli, the bats responded to the dishabituation stimuli independent of affect intensity, emphasising the attention-grabbing function of this call type. For response call stimuli, the bats responded to a high affect intensity test stimulus after experiencing stimuli of low affect intensity, but transferred habituation to a low affect intensity test stimulus after experiencing stimuli of high affect intensity. This transfer of habituation was not due to over-habituation as the bats responded to a frequency-shifted control stimulus. A direct comparison confirmed the asymmetric response behaviour in the reciprocal experiments.Conclusions
Thus, the present study provides not only evidence for a discrimination of affect intensity, but also for an evaluation of stimulus salience, suggesting that basic assessment mechanisms involved in the perception of emotion are an ancestral trait in mammals.48.
Satapathy AK Kulczyk AW Ghosh S van Oijen AM Richardson CC 《The Journal of biological chemistry》2011,286(39):34468-34478
The DNA helicase encoded by gene 4 of bacteriophage T7 assembles on single-stranded DNA as a hexamer of six identical subunits with the DNA passing through the center of the toroid. The helicase couples the hydrolysis of dTTP to unidirectional translocation on single-stranded DNA and the unwinding of duplex DNA. Phe(523), positioned in a β-hairpin loop at the subunit interface, plays a key role in coupling the hydrolysis of dTTP to DNA unwinding. Replacement of Phe(523) with alanine or valine abolishes the ability of the helicase to unwind DNA or allow T7 polymerase to mediate strand-displacement synthesis on duplex DNA. In vivo complementation studies reveal a requirement for a hydrophobic residue with long side chains at this position. In a crystal structure of T7 helicase, when a nucleotide is bound at a subunit interface, Phe(523) is buried within the interface. However, in the unbound state, it is more exposed on the outer surface of the helicase. This structural difference suggests that the β-hairpin bearing the Phe(523) may undergo a conformational change during nucleotide hydrolysis. We postulate that upon hydrolysis of dTTP, Phe(523) moves from within the subunit interface to a more exposed position where it contacts the displaced complementary strand and facilitates unwinding. 相似文献
49.
Siddhartha Sankar Satapathy Bhesh Raj Powdel Malay Dutta Alak Kumar Buragohain Suvendra Kumar Ray 《Journal of molecular evolution》2014,78(1):13-23
The fourfold degenerate site (FDS) in coding sequences is important for studying the effect of any selection pressure on codon usage bias (CUB) because nucleotide substitution per se is not under any such pressure at the site due to the unaltered amino acid sequence in a protein. We estimated the frequency variation of nucleotides at the FDS across the eight family boxes (FBs) defined as Um(g), the unevenness measure of a gene g. The study was made in 545 species of bacteria. In many bacteria, the Um(g) correlated strongly with Nc′—a measure of the CUB. Analysis of the strongly correlated bacteria revealed that the U-ending codons (GGU, CGU) were preferred to the G-ending codons (GGG, CGG) in Gly and Arg FBs even in the genomes with G+C % higher than 65.0. Further evidence suggested that these codons can be used as a good indicator of selection pressure on CUB in genomes with higher G+C %. 相似文献
50.