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991.
Uwe Schlattner Malgorzata Tokarska-Schlattner Sacnicte Ramirez Yulia Y. Tyurina Andrew A. Amoscato Dariush Mohammadyani Zhentai Huang Jianfei Jiang Naveena Yanamala Amal Seffouh Mathieu Boissan Raquel F. Epand Richard M. Epand Judith Klein-Seetharaman Marie-Lise Lacombe Valerian E. Kagan 《The Journal of biological chemistry》2013,288(1):111-121
The nucleoside diphosphate kinase Nm23-H4/NDPK-D forms symmetrical hexameric complexes in the mitochondrial intermembrane space with phosphotransfer activity using mitochondrial ATP to regenerate nucleoside triphosphates. We demonstrate the complex formation between Nm23-H4 and mitochondrial GTPase OPA1 in rat liver, suggesting its involvement in local and direct GTP delivery. Similar to OPA1, Nm23-H4 is further known to strongly bind in vitro to anionic phospholipids, mainly cardiolipin, and in vivo to the inner mitochondrial membrane. We show here that such protein-lipid complexes inhibit nucleoside diphosphate kinase activity but are necessary for another function of Nm23-H4, selective intermembrane lipid transfer. Mitochondrial lipid distribution was analyzed by liquid chromatography-mass spectrometry using HeLa cells expressing either wild-type Nm23-H4 or a membrane binding-deficient mutant at a site predicted based on molecular modeling to be crucial for cardiolipin binding and transfer mechanism. We found that wild type, but not the mutant enzyme, selectively increased the content of cardiolipin in the outer mitochondrial membrane, but the distribution of other more abundant phospholipids (e.g. phosphatidylcholine) remained unchanged. HeLa cells expressing the wild-type enzyme showed increased accumulation of Bax in mitochondria and were sensitized to rotenone-induced apoptosis as revealed by stimulated release of cytochrome c into the cytosol, elevated caspase 3/7 activity, and increased annexin V binding. Based on these data and molecular modeling, we propose that Nm23-H4 acts as a lipid-dependent mitochondrial switch with dual function in phosphotransfer serving local GTP supply and cardiolipin transfer for apoptotic signaling and putative other functions. 相似文献
992.
Martina G. Ramirez Celina M. Oliveri Dina Mismar Amy Barsoum Jasmin Abdulla 《Invertebrate reproduction & development.》2013,57(2):96-103
Male Mastophora cornigera exit egg sacs as adults, which allowed us to determine spiderling sex ratios and patterns of maternal investment in this species. We collected 15 egg sacs produced by seven mothers, which yielded 1945 emergent spiderlings which were sexed, 1850 of which were weighed. Two emergent broods were significantly male and female biased and were unaffected by pre-emergence mortality. The weights of male and female spiderlings differed in eight broods, with males and females being heavier in four cases each. Five of these broods were derived from multiple egg sac sets produced by one mother, and in each case, the total mean male and female spiderling weights for all broods in a set were biased in the same direction as the biased brood(s) within that set. Mean emergent spiderling weight was independent of brood size and sex ratio for both males and females. Despite such independence, sex allocation in M. cornigera can favor sons, daughters, or both equally, and by numbers, by weight, or both at once. The proximate mechanisms and adaptive significance of such variability is unknown. We also review evidence for gender-biased allocations in arachnid offspring and suggested mechanisms for their applicability to M. cornigera. 相似文献
993.
David Requena Ana Chumbe Michael Torres Ofelia Alzamora Manuel Ramirez Hugo Valdivia-Olarte Andres Hazaet Gutierrez Ray Izquierdo-Lara Luis Enrique Saravia Milagros Zavaleta Luis Tataje-Lavanda Ivan Best Manolo Fernández-Sánchez Eliana Icochea Mirko Zimic Manolo Fernández-Díaz 《Bioinformation》2013,9(10):528-536
Background: Avibacterium paragallinarum, the causative agent of infectious coryza, is a highly
contagious respiratory acute disease of poultry, which affects commercial chickens, laying hens and broilers worldwide.
Methodology: In this study, we performed the whole genome sequencing, assembly and annotation of a Peruvian
isolate of A. paragallinarum. Genome was sequenced in a 454 GS FLX Titanium system. De novo assembly was
performed and annotation was completed with GS De Novo Assembler 2.6 using the H. influenzae str. F3031 gene model.
Manual curation of the genome was performed with Artemis. Putative function of genes was predicted with Blast2GO.
Virulence factors were identified by comparison with the Virulence Factor Database.
Results: The genome obtained has a length of 2.47 Mb with 40.66% of GC content. Seventy five large contigs
(>500 nt) were obtained, which comprised 1,204 predicted genes. All the contigs are available in Genbank
[GenBank: PRJNA64665]. A total of 103 virulence factors, reported in the Virulence Factor Database, were
found in A. paragallinarum. Forty four of them are present in 7 species of Haemophilus, which are related
with pathogenesis, virulence and host immune system evasion. A tetracycline-resistance associated transposon
(Tn10), was found in A. paragallinarum, possibly acting as a defense mechanism.
Discussion and conclusion: The availability of A. paragallinarum genome represents an important source of information for
the development of diagnostic tests, genotyping, and novel antigens for potential vaccines against infectious coryza.
Identification of virulence factors contributes to better understanding the pathogenesis, and planning efforts for prevention
and control of the disease. 相似文献
994.
E. E. Becerril‐Garcia D. Petatan‐Ramirez I. Ortiz‐Aguirre A. Ayala‐Bocos 《Journal of fish biology》2018,92(4):1207-1210
This is the first record of the Pacific seahorse Hippocampus ingens at a northern oceanic island from the eastern Pacific Ocean. The photographic record of the juvenile female H. ingens was made in November 2015 during a cage diving trip at Guadalupe Island, Mexico. The presence of H. ingens in this area could be related to long distance dispersal mechanisms, as has been observed in other species of seahorses. 相似文献
995.
Early postnatal development of electrophysiological and histological properties of sensory sural nerves in male rats that were maternally deprived and artificially reared: Role of tactile stimulation 下载免费PDF全文
Rene Zempoalteca Mercedes G. Porras Suelem Moreno‐Pérez Gabriela Ramirez‐Funez Elsa L. Aguirre‐Benítez Margarita González del Pliego Silvia Mariscal‐Tovar Maria E. Mendoza‐Garrido Kurt Leroy Hoffman Ismael Jiménez‐Estrada Angel I. Melo 《Developmental neurobiology》2018,78(4):351-362
Early adverse experiences disrupt brain development and behavior, but little is known about how such experiences impact on the development of the peripheral nervous system. Recently, we found alterations in the electrophysiological and histological characteristics of the sensory sural (SU) nerve in maternally deprived, artificially reared (AR) adult male rats, as compared with maternally reared (MR) control rats. In the present study, our aim was to characterize the ontogeny of these alterations. Thus, male pups of four postnatal days (PND) were (1) AR group, (2) AR and received daily tactile stimulation to the body and anogenital region (AR‐Tactile group); or (3) reared by their mother (MR group). At PND 7, 14, or 21, electrophysiological properties and histological characteristics of the SU nerves were assessed. At PND 7, the electrophysiological properties and most histological parameters of the SU nerve did not differ among MR, AR, and AR‐Tactile groups. By contrast, at PND 14 and/or 21, the SU nerve of AR rats showed a lower CAP amplitude and area, and a significant reduction in myelin area and myelin thickness, which were accompanied by a reduction in axon area (day 21 only) compared to the nerves of MR rats. Tactile stimulation (AR‐Tactile group) partially prevented most of these alterations. These results suggest that sensory cues from the mother and/or littermates during the first 7–14 PND are relevant for the proper development and function of the adult SU nerve. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 78: 351–362, 2018 相似文献
996.
Julian Ramirez‐Villegas Alexandre B. Heinemann Adriano Pereira de Castro Flávio Breseghello Carlos Navarro‐Racines Tao Li Maria C. Rebolledo Andrew J. Challinor 《Global Change Biology》2018,24(5):2035-2050
Rice is the most important food crop in the developing world. For rice production systems to address the challenges of increasing demand and climate change, potential and on‐farm yield increases must be increased. Breeding is one of the main strategies toward such aim. Here, we hypothesize that climatic and atmospheric changes for the upland rice growing period in central Brazil are likely to alter environment groupings and drought stress patterns by 2050, leading to changing breeding targets during the 21st century. As a result of changes in drought stress frequency and intensity, we found reductions in productivity in the range of 200–600 kg/ha (up to 20%) and reductions in yield stability throughout virtually the entire upland rice growing area (except for the southeast). In the face of these changes, our crop simulation analysis suggests that the current strategy of the breeding program, which aims at achieving wide adaptation, should be adjusted. Based on the results for current and future climates, a weighted selection strategy for the three environmental groups that characterize the region is suggested. For the highly favorable environment (HFE, 36%–41% growing area, depending on RCP), selection should be done under both stress‐free and terminal stress conditions; for the favorable environment (FE, 27%–40%), selection should aim at testing under reproductive and terminal stress, and for the least favorable environment (LFE, 23%–27%), selection should be conducted for response to reproductive stress only and for the joint occurrence of reproductive and terminal stress. Even though there are differences in timing, it is noteworthy that stress levels are similar across environments, with 40%–60% of crop water demand unsatisfied. Efficient crop improvement targeted toward adaptive traits for drought tolerance will enhance upland rice crop system resilience under climate change. 相似文献
997.
Effect of partially replacing a barley‐based concentrate with flaxseed‐based products on the rumen bacterial population of lactating Holstein dairy cows 下载免费PDF全文
E. Castillo‐Lopez J. Moats N.D. Aluthge H.A. Ramirez Ramirez D.A. Christensen T. Mutsvangwa G.B. Penner S.C. Fernando 《Journal of applied microbiology》2018,124(1):42-57
Aims
The effects of partial replacement of a barley‐based concentrate with flaxseed‐based products on the rumen bacterial population of lactating Holstein dairy cows were evaluated.Methods and Results
Treatments fed were CONT, a normal diet that included barley silage, alfalfa hay and a barley‐based concentrate that contained no flaxseed or faba beans; FLAX, inclusion of a nonextruded flaxseed‐based product containing 55·0% flaxseed, 37·8% field peas and 6·9% alfalfa; EXT, similar to FLAX, but the product was extruded and EXTT, similar to FLAX, but product was extruded and field peas were replaced by high‐tannin faba beans. The rumen bacterial population was evaluated by utilizing 16S rRNA gene sequencing. Most abundant phyla, families and genera were unaffected. However, some taxa were affected; for example, unsaturated fatty acid content was negatively correlated with Clostridiaceae, and tannin content was negatively correlated with BS11 and Paraprevotellaceae.Conclusions
Predominant rumen bacterial taxa were not affected, but the abundance of some taxa found in lower proportions shifted, possibly due to sensitivity to unsaturated fatty acids or tannins.Significance and Impact of the Study
Flaxseed‐based products were effective for partially replacing barley‐based concentrate in rations of lactating dairy cows. No negative effects of these products were observed on the abundance of predominant rumen bacterial taxa, with only minor shifts in less abundant bacteria. 相似文献998.
Luigia Petti Paola Karina Sanchez Ramirez Marzia Traverso Cassia Maria Lie Ugaya 《The International Journal of Life Cycle Assessment》2018,23(3):569-580
Purpose
The main purpose of this study is to present an implementation of the subcategory assessment method (SAM) to the life cycle of an Italian variety of tomato called “Cuore di Bue” produced by an Italian cooperative. The case study was used to use the methodology proposed in compliance with the guidelines of social life cycle assessment (S-LCA) in order to highlight issues for the improvement of SAM. A summary of strengths and weaknesses of the methodology as well as the social performance of the considered Italian tomato is an important result of this case study.Methods
The methodology used is based on SAM. The UNEP/SETAC guidelines of S-LCA and the complementary methodological sheets were used as main references to carry out SAM, and it was used to assess the social performances of Cuore di Bue. The focus was on the assessment of the following three out of five stakeholder groups presented in the guidelines: workers, local community and consumers. Specific questionnaires have been developed to collect the inventory data related to each stakeholder group and along the product life cycle.Results and discussion
SAM of Cuore di Bue showed a range of values, between 2 and 3 (C-B) for consumer stakeholder group and mainly 3 (B) for the local community and worker stakeholders. Because the best performance (A) is related to a numerical value of 4, better performances were not identified, owing to no propagation of actions in the value chain. The collective bargaining, transparency, feedback mechanism and privacy are the subcategories with the worst performance, but at the same time with more potential for improvements.Conclusions
The implementation of SAM on Cuore di Bue allowed us to demonstrate how SAM transforms qualitative data into semi-quantitative information through a score scale that can help a decision maker achieve a product overview. SAM has been implemented on Cuore di Bue; the product assessment, the strengths and weaknesses of the methodology are identified and discussed as well. It has been possible to present the best and worst performances in product life cycle, by identifying the phase or the subcategories with good or bad performance. However, in this case study, as the same company owns most of the product life cycle taken into account, the majority of social performances are identical, and this may represent a limit of the methodology or that more organisations along the life cycle must be taken into account (for example, energy, distribution).999.
1000.
The epidemiology of cryptosporidiosis, an infection caused by several genotypically and phenotypically diverse Cryptosporidium species, has been dynamically changing over the past decade from that of a rare, largely asymptomatic infection to an acute enteric disease of animals and humans. In this review, the current understanding of factors (biology and epidemiology) contributing to the emergence of cryptosporidiosis in animals, including parasite biology, genetic diversity, environmental spread, livestock production trends, presence of the parasite in livestock and companion animals, and potential risk of transmission from animals to humans is highlighted. Potential control measures and the role of veterinary and medical professionals in the prevention of cryptosporidiosis are also discussed. 相似文献