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71.
Water-relations parameters were measured on sections of secondaryphloem from red oak (Quercus borealis michx. f.) and white ash(Fraxinus americana var. biltmoreana [Beadle] J. Wright) usinga linear displacement transducer. Changes in tissue thicknessin response to changes in the osmotic pressure of the bathingsolution were used to calculate the volumetric elastic modulusplus osmotic pressure (v + ) of the tissue, and an applied forcemethod was used to estimate the time constant for water equilibration(T). The hydraulic conductivity of the cell membranes (Lp) wascalculated utilizing v + and r values. The time-dependent behaviour of the tissue was much more complexthan originally expected. A correction for a time-dependentprocess that we call drift was required to obtainnumbers for v + . Furthermore, v + was calculated on two assumptionsin order to relate changes in tissue dimensions to sieve elementparameters. In the first case, a lower limit for v + of thesieve elements was determined by attributing all changes intissue dimensions to these cells. For red oak the average v+ on this assumption is 72 bars. Assuming that all cell typeswere equally responsible for the changes in tissue dimensionsresulted in an v + value of 192 bars for oak. If v + and rare the same for all cells in the tissue, Lp for the sieve elementsof oak is 9.6 x 108 cm s1 bar1. Exudationfrom the sieve elements of white ash during excision of thephloem led to artificially high values of v + for that species. Quercus borealis michx. f., Fraxinus americana var, biltmoreana (Beadle) J. Wright, red oak, white ash, water relations, phloem, volumetric elastic modulus, membrane hydraulic conductivity 相似文献
72.
SYNOPSIS. The mechanisms of ferritin uptake and digestion differ in bloodstream and culture forms of Trypanosoma brucei. Ferritin enters bloodstream forms from the flagellar pocket by pinocytosis in large spiny-coated vesicles. These vesicles become continuous with straight tubular extensions of a complex, mostly tubular, collecting membrane system where ferritin is concentrated. From the collecting membrane system the tracer enters large digestive vacuoles. Small spiny-coated vesicles, which never contain ferritin, are found in the Golgi region, fusing with the collecting membrane system, and around the flagellar pocket. Acid phosphatase activity is present in some small spiny-coated vesicles which may represent primary lysosomes. This enzymic activity is also found in the flagellar pocket, pinocytotic vesicles, the collecting membrane system, the Golgi (mature face), and digestive vacuoles of bloodstream forms. About 50% of the acid phosphatase activity of blood forms is latent. The remaining nonlatent activity is firmly cell-associated and probably represents activity in the flagellar pocket. The structures involved in ferritin uptake and digestion are larger and more active in the short stumpy than in the long slender bloodstream forms. The short stumpy forms also have more autophagic vacuoles. No pinocytotic large, spiny-coated vesicles or Golgi-derived, small spiny-coated vesicles are seen in culture forms. Ferritin leaves the flagellar pocket of these forms and enters small smooth cisternae located just beneath bulges in the pocket membrane. The tracer then passes through a cisternal collecting membrane network, where it is concentrated, and then into multivesicular bodies. In the culture forms, acid phosphatase activity is localized in the cisternal system, multivesicular bodies, the Golgi (mature face), and small vesicles in the Golgi and cisternal regions. The flagellar pocket has no acid phosphatase activity, and almost all the activity is latent in these forms. The culture forms do not release acid phosphatase into culture medium during 4 days growth. Uptake of ferritin by all forms is almost completely inhibited by low temperature. These differences among the long slender and short stumpy bloodstream forms and culture forms are undoubtedly adaptive and reflect different needs of the parasite in different life cycle stages. 相似文献
73.
SUSAN L. ANKROM BILL CHOBOTAR JOHN V. ERNST† 《The Journal of eukaryotic microbiology》1975,22(3):317-323
SYNOPSIS. The life cycle of Eimeria ferrisi is described from experimentally infected Mus musculus. The prepatent period was 3 days and the patent period was 3–4 days. The endogenous stages were found only in the cecum and colon. Three generations of schizonts were found. Mature 1st-generation schizonts first seen 24 hr postinoculation (PI) measured 10.9 (7–14) × 10.2 (6–13) μm and had 9.6 (7–14) merozoites. Some 2nd-generation schizonts had uninucleate merozoites and others had multinucleate merozoites. The former were first seen in small numbers 36 hr PI and were most abundant 48 hr PI. They measured 9.6 (5–13) × 7.9 (6–12) μm and had 18 (6–25) merozoites. Schizonts with multinucleate merozoites were seen 72 hr PI. Mature 3rd-generation schizonts were seen 72 hr PI. They measured 14.0 (12–18) × 11-0 (9–13) μm and had 12.5 (5–16) merozoites. Macrogamonts were first seen in 72 hr sections. Each young macrogamont had a large nucleus with a prominent nucleolus. Only one type of cytoplasmic granule appeared to be involved in the formation of the oocyst wall. Mature macrogamonts were 11.0 (5–14) × 10.0 (6–13) μm. Crescent-shaped bodies were observed in the parasitophorous vacuole of trophozoites and young macrogamonts. Early microgamonts were first recognized at 96 hr by the presence of darkly stained and irregularly shaped nuclei. Usually, mature microgametes were arranged in long, narrow whorls at the periphery of the microgamont or in whorls at the surface of 2–5 compartments. 相似文献
74.
Amphetamine Response in Rat after Dopamine Neurone Destruction 总被引:2,自引:0,他引:2
AMPHETAMINE increases spontaneous locomotor activity and induces stereotyped motor responses in rats. The magnitude of these two behavioural responses varies according to the dose and which optical isomer of the drug is used1,2. Amphetamine is known to influence the uptake and release of catecholamines in the brain both in vitro3 and in vivo4,5 and the finding that inhibition of catecholamine synthesis by α methyl-p-tyrosine abolishes the behavioural effect of the drug6,7 suggests that a release of catecholamines may mediate these effects. The amine transmitters noradrenaline (NA) and dopamine (DA) are localized in different anatomical systems in the brain8,9 and attempts have been made to correlate the specific behavioural effects of amphetamine with one or other of these aminergic systems. Taylor and Snyder, on the basis of comparisons of the potency of d- and l-amphetamine in behavioural and biochemical tests, suggested that a release of NA mediates the locomotor activity and DA the stereotypy responses. We have attempted to pursue these hypotheses with lesions to aminergic pathways. 相似文献
75.
A new lizard-like reptile (Diapsida: Lepidosauromorpha) from the Middle Jurassic of England 总被引:1,自引:0,他引:1
SUSAN E. EVANS 《Zoological Journal of the Linnean Society》1991,103(4):391-412
The Middle Jurassic (Late Bathonian) Mammal Bed at Kirtlington, Oxfordshire, has yielded a rich assemblage of small vertebrates including mammals, frogs, salamanders and small reptiles. This paper describes the skull of a new diapsid reptile, Marmorelta oxoniensis, which was common within the fauna. The skull is gracile with large orbits and relatively long external nares. The frontals and parietals are single in the adult and almost separated on the roof of the skull by the postfrontals. The temporal region shows a mosaic of primitive and derived character states. The quadrate was firmly held to the skull by a large squamosal and a small, free, quadratojugal was retained. However, reduction of the anterior process of the quadratojugal and of the posterior process of the jugal left the lower temporal fenestra broadly open. Comparison with other diapsids leads to the conclusion that Marmoretta was a lepidosauromorph, most probably the sister taxon of Lepidosauria. 相似文献
76.
ROBERT E. HILLMAN SUSAN E. FORD HAROLD H. HASKIN 《The Journal of eukaryotic microbiology》1990,37(5):364-368
ABSTRACT. A new species of ascetosporan parasitizing tissues of woodboring mollusks of the genus Teredo , including T. navalis Linnaeus, T. furcifera von Martens, and T. bartschi Clapp, is described from light and transmission electron microscopical observations. The new species is assigned the name Minchinia teredinis sp. n. (Phylum Ascetospora, Class Stellatosporea, Order Balanosporida, Family Haplosporidiidae). Plasmodia, sporonts, sporocysts, and mature spores are found in all host tissues, but primarily in the gill. Spores are obovate, operculate, and characterized by four projections from the epispore membrane. The species is found from Long Island Sound to Virginia on the east coast of the United States. The parasite causes extensive damage to host tissues and is correlated with reductions in host populations. 相似文献
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