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21.
João Gonçalves Sofia Nolasco Rute Nascimento Mónica Lopez Fanarraga Juan Carlos Zabala Helena Soares 《EMBO reports》2010,11(3):194-200
In animal cells the centrosome is positioned at the cell centre in close association with the nucleus. The mechanisms responsible for this are not completely understood. Here, we report the first characterization of human TBCC‐domain containing 1 (TBCCD1), a protein related to tubulin cofactor C. TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE‐1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1‐depleted cells are larger, less efficient in primary cilia assembly and their migration is slower in wound‐healing assays. However, the major microtubule‐nucleating activity of the centrosome is not affected by TBCCD1 silencing. We propose that TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization. 相似文献
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Molecular evolution and the role of oxidative stress in the expansion and functional diversification of cytosolic glutathione transferases 总被引:1,自引:0,他引:1
Rute R da Fonseca Warren E Johnson Stephen J O'Brien Vítor Vasconcelos Agostinho Antunes 《BMC evolutionary biology》2010,10(1):281
Background
Cytosolic glutathione transferases (cGST) are a large group of ubiquitous enzymes involved in detoxification and are well known for their undesired side effects during chemotherapy. In this work we have performed thorough phylogenetic analyses to understand the various aspects of the evolution and functional diversification of cGSTs. Furthermore, we assessed plausible correlations between gene duplication and substrate specificity of gene paralogs in humans and selected species, notably in mammalian enzymes and their natural substrates. 相似文献23.
Cristina Branquinho Paula MatosAna Rute Vieira Maria Margarida Prestello Ramos 《Environmental and Experimental Botany》2011,72(1):84-92
The relative impact of lichen photobiont and mycobiont was evaluated by submitting nine lichen species with: (i) different photobiont types; (ii) different lichen growth forms; and (iii) different nutrients, pH, humidity preferences; to a range of Cu concentrations (μM) supplied in repeated cycles to simulate the natural process of uptake under field conditions. The physiological performance of the photosystem II photochemical reactions was measured using Fv/Fm and the metabolic activity of the mycobiont was evaluated using ergosterol and intracellular K-loss as indicators. Lichens with higher cation exchange capacity showed higher intracellular Cu uptake and their ecology seemed to be associated with low-nutrient environments. Thus the wall and external matrix, mainly characteristic of the mycobiont partner, cannot be ignored as the first site of interaction of metals with lichens. No common intracellular Cu concentration threshold was found for the physiological impacts observed in the different species. Most physiological effects of Cu uptake in sensitive lichens occurred for intracellular Cu below 200 μg/g dw whereas more tolerant species were able to cope with intracellular Cu at least 3 times higher. Cyanobacterial lichens showed to be more sensitive to Cu uptake than green-algal lichens. Within the Trebouxia lichens, different species showed different sensitivities to Cu uptake, suggesting that the mycobiont may change the microenvironment close to the photobiont partner providing different degrees of protection. Despite the fact that the photobiont is the productive partner, the metabolic activity of the mycobiont of lichen species adapted to environments rich in nutrients, showed to be more sensitive to Cu uptake than the photochemical performance of the photobiont. 相似文献
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Agnieszka M. Szemiel Andres Merits Richard J. Orton Oscar A. MacLean Rute Maria Pinto Arthur Wickenhagen Gauthier Lieber Matthew L. Turnbull Sainan Wang Wilhelm Furnon Nicolas M. Suarez Daniel Mair Ana da Silva Filipe Brian J. Willett Sam J. Wilson Arvind H. Patel Emma C. Thomson Massimo Palmarini Alain Kohl Meredith E. Stewart 《PLoS pathogens》2021,17(9)
Remdesivir (RDV), a broadly acting nucleoside analogue, is the only FDA approved small molecule antiviral for the treatment of COVID-19 patients. To date, there are no reports identifying SARS-CoV-2 RDV resistance in patients, animal models or in vitro. Here, we selected drug-resistant viral populations by serially passaging SARS-CoV-2 in vitro in the presence of RDV. Using high throughput sequencing, we identified a single mutation in RNA-dependent RNA polymerase (NSP12) at a residue conserved among all coronaviruses in two independently evolved populations displaying decreased RDV sensitivity. Introduction of the NSP12 E802D mutation into our SARS-CoV-2 reverse genetics backbone confirmed its role in decreasing RDV sensitivity in vitro. Substitution of E802 did not affect viral replication or activity of an alternate nucleoside analogue (EIDD2801) but did affect virus fitness in a competition assay. Analysis of the globally circulating SARS-CoV-2 variants (>800,000 sequences) showed no evidence of widespread transmission of RDV-resistant mutants. Surprisingly, we observed an excess of substitutions in spike at corresponding sites identified in the emerging SARS-CoV-2 variants of concern (i.e., H69, E484, N501, H655) indicating that they can arise in vitro in the absence of immune selection. The identification and characterisation of a drug resistant signature within the SARS-CoV-2 genome has implications for clinical management and virus surveillance. 相似文献
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Patrícia Pečnerová Genís Garcia-Erill Xiaodong Liu Casia Nursyifa Ryan K. Waples Cindy G. Santander Liam Quinn Peter Frandsen Jonas Meisner Frederik Filip Stæger Malthe Sebro Rasmussen Anna Brüniche-Olsen Christian Hviid Friis Jørgensen Rute R. da Fonseca Hans R. Siegismund Anders Albrechtsen Rasmus Heller Ida Moltke Kristian Hanghøj 《Current biology : CB》2021,31(9):1862-1871.e5
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Isolation and characterization of ten microsatellite loci in stingless bee Trigona spinipes (Apidae: Meliponini) 下载免费PDF全文
Leandro Rodrigues Santiago Fabiana Curtopassi Pioker‐Hara Flávio de Oliveira Francisco Rute Magalhães Brito Paulo Henrique Pereira Gonçalves Alayne Magalhães Trindade Domingues‐Yamada Maria Cristina Arias 《Entomological Science》2015,18(1):138-140
Stingless bees are the most abundant pollinators of Brazilian tropical flora. Trigona spinipes has some of the largest colonies of any stingless bee species found in several types of environment. This work describes the isolation and characterization of microsatellite loci for this species. A microsatellite‐enriched genomic library was constructed and ten primer pairs were designed for T. spinipes. The primers were tested in 20 unrelated individuals. The mean number of alleles was 8.10 and mean observed and expected heterozygosity were 0.655 and 0.680, respectively. Primers were also tested in cross‐species amplification and five loci were successfully amplified in Trigona chanchamayoensis, Trigona hyalinata, Tetragonisca angustula, Partamona mulata and Frieseomelitta varia. The microsatellite primers described herein will be useful for evaluating genetic variability and gaining a better understanding of the population structure of T. spinipes as well as other species of stingless bees. 相似文献
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Boonstra A Rajsbaum R Holman M Marques R Asselin-Paturel C Pereira JP Bates EE Akira S Vieira P Liu YJ Trinchieri G O'Garra A 《Journal of immunology (Baltimore, Md. : 1950)》2006,177(11):7551-7558
We have previously reported that mouse plasmacytoid dendritic cells (DC) produce high levels of IL-12p70, whereas bone marrow-derived myeloid DC and splenic DC produce substantially lower levels of this cytokine when activated with the TLR-9 ligand CpG. We now show that in response to CpG stimulation, high levels of IL-10 are secreted by macrophages, intermediate levels by myeloid DC, but no detectable IL-10 is secreted by plasmacytoid DC. MyD88-dependent TLR signals (TLR4, 7, 9 ligation), Toll/IL-1 receptor domain-containing adaptor-dependent TLR signals (TLR3, 4 ligation) as well as non-TLR signals (CD40 ligation) induced macrophages and myeloid DC to produce IL-10 in addition to proinflammatory cytokines. IL-12p70 expression in response to CpG was suppressed by endogenous IL-10 in macrophages, in myeloid DC, and to an even greater extent in splenic CD8alpha(-) and CD8alpha(+) DC. Although plasmacytoid DC did not produce IL-10 upon stimulation, addition of this cytokine exogenously suppressed their production of IL-12, TNF, and IFN-alpha, showing trans but not autocrine regulation of these cytokines by IL-10 in plasmacytoid DC. 相似文献
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Maria Rosário Sambo Maria Jesus Trovoada Carla Benchimol Vatúsia Quinhentos Lígia Gon?alves Rute Velosa Maria Isabel Marques Nuno Sepúlveda Taane G. Clark Stefan Mustafa Oswald Wagner António Coutinho Carlos Penha-Gon?alves 《PloS one》2010,5(6)