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The hydrophobic membrane-spanning sequences of the gp52 glycoprotein are required for the pathogenicity of Friend spleen focus-forming virus. 总被引:3,自引:1,他引:2 下载免费PDF全文
Friend spleen focus-forming virus (SFFV) codes for a transport-defective envelope glycoprotein designated gp52, which is responsible for the leukemogenic properties of the virus. gp52 is a monotopic integral membrane protein anchored in the membrane by a stretch of hydrophobic amino acid residues located near the carboxy terminus of the molecule. We have constructed a mutant SFFV envelope gene in which the sequences that code for the hydrophobic membrane-spanning domain have been deleted, and we expressed this gene by using recombinant vaccinia virus vectors or retroviral vectors. The mutant SFFV envelope gene was found to encode a truncated glycoprotein (gp52t) which was also transport defective; a majority of gp52t remained cell associated, while a small proportion of the molecules underwent oligosaccharide processing. The processed form of gp52t was secreted from the cells. Retroviral vectors carrying the mutant SFFV envelope gene were found to be nonpathogenic in adult mice. These results indicate that the hydrophobic membrane-spanning region of gp52 is required for pathogenicity of SFFV and suggest that these sequences may play a role in signal transduction. The results also indicate that the transport defect of SFFV gp52 is due to structural features of the ectodomain of the molecule. 相似文献
34.
Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes. 总被引:71,自引:0,他引:71
Xenopus eggs contain two distinct cdc2 homologs of 34 and 32 kd. We show that the 32 kd cdc2 protein, like the 34 kd protein, is a kinase. However, unlike the 34 kd homolog, the 32 kd cdc2 kinase activity does not decrease dramatically at the end of mitosis. The 32 kd protein does not associate with mitotic cyclins B1 and B2 but does associate with cyclin A and a novel doublet of proteins of 54 kd that may regulate its activity. We also show that depletion of the 32 kd cdc2 homolog from a Xenopus extract blocks DNA replication, but does not inhibit entry into mitosis. By contrast, depletion of the 34 kd cdc2 homolog or absence of mitotic cyclins from an extract does not inhibit replication, but does block entry into mitosis. Our results indicate that in higher eukaryotes, DNA replication (G1-S) and mitosis (G2-M) may be controlled by distinctly different cdc2 proteins. 相似文献
35.
Rong-Xiang Fang Zhen Pang Dong-Ming Gao Ke-Qiang Mang Nam-Hai Chua 《Plant molecular biology》1991,17(6):1255-1257
36.
M. Yang Fang Ming J. Hubble A. D. Lockett R. R. Rathbone J. A. Howell 《Biotechnology Techniques》1992,6(5):409-412
A simple thermal monitoring technique has been successfully applied to an adsorption system using a novel ion exchanger with
a large internal void volume (voidage) which can be operated at high superficial velocity (SV). Temperature changes resulting
from heats of adsorption could be followed effectively using semi-conductor thermistor devices inserted into the resin through
the column wall. Results show that, despite the high feed rates adopted, the thermal signals generated can be consistently
related to the position of the breakthrough front within the bed. 相似文献
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中药山豆根的研究进展 总被引:3,自引:1,他引:2
本文对中药山豆根进行了本草考证,并概括总结了半个世纪以来国内外对山豆根(广豆根)和北豆根在生药学研究、化学成分、药理作用与临床应用等方面的研究成果,为山豆根的进一步研究提供参考。全文附参考文献105篇。 相似文献
39.
中国大鲵视网膜的光镜和扫描电镜研究 总被引:7,自引:0,他引:7
用光镜和扫描电镜观察了大鲵视网膜各类细胞的形态及分布, 对视细胞和节细胞进行计数。视网腊中三个核层及两个网状层分布均匀,无中央凹。每张视网膜的视细胞总数约130000,节细胞约8000,视杆与视锥之比为8.5:1。扫描电镜下,视杆外节表面的小叶间沟清晰;视杆视锥外节均有从内节伸出的20-30条萼状突起;核周体表面亦有20-30条细胞质突起。文中还报道了幼体视细胞的形态及密度。讨论了上述结构的机能。 相似文献
40.
Carcinogenicity of catechol estrogens in Syrian hamsters 总被引:30,自引:0,他引:30
Estradiol and other estrogens induce renal carcinoma in male Syrian hamsters. The mechanism of carcinogenesis still remains unclear. Activation of estrogens to catechol metabolites has in the past been postulated to play a role in estrogen-induced carcinogenesis. Therefore, the carcinogenic activity of catechol estrogens was investigated. After 175 days of treatment, 4-hydroxyestradiol was found to be as carcinogenic as estradiol in male Syrian hamsters (4/5 and 4/5 animals with kidney tumors, respectively). Animals treated with 2-hydroxyestradiol (0/5) or 2-methoxyestradiol (0/6) did not develop renal carcinoma. The catechol estrogens failed to be mutagenic in the Ames test (reversions of his- S. typhimurium to histidine prototrophy in the TA 100 strain). The lack of carcinogenic activity of 2-hydroxyestradiol was not due to a failure to stimulate estrogen-dependent tumor growth. Growth of H-301 cells, an estrogen-dependent hamster kidney tumor cell line, was supported in vivo by estrogens in the following order: estradiol greater than 4-hydroxyestradiol greater than 2-hydroxyestradiol. Stimulation of tumor growth by 2-methoxyestradiol was not detected. It was concluded that the carcinogenic activity of 4-hydroxyestradiol was consistent with a role of catechol metabolites in estrogen-induced carcinogenesis. However, the intrinsic carcinogenic or hormonal activity of 2-hydroxyestradiol probably can not be assessed accurately in vivo because of its rapid methylation and metabolic clearance. 相似文献