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71.
Kolchinskiĭ AM Griadunov DA Lysov IuP Mikhaĭlovich VM Nasedkina TV Turygin AIu Rubina AIu Barskiĭ VE Zasedatelev AS 《Molekuliarnaia biologiia》2004,38(1):5-16
The review describes the history of creation and development of the microchip technology and its role in the human genome project in Russia. The emphasis is placed on the three-dimensional gel-based microchips developed at the Center of Biological Microchips headed by A.D. Mirzabekov since 1988. The gel-based chips of the last generation, IMAGE chips (Immobilized Micro Array of Gel Elements), have a number of advantages over the previous versions. The microchips are manufactured by photo-initiated copolymerization of gel components and immobilized molecules (DNA, proteins, and ligands). This ensures an even distribution of the immobilized probe throughout the microchip gel element with a high yield (about 50% for oligonucleotides). The use of methacrylamide as a main component of the polymerization mixture resulted in a substantial increase of gel porosity without affecting its mechanical strength and stability, which allowed one to work with the DNA fragments of up to 500 nt in length, as well as with rather large protein molecules. At present, the gel-based microchips are widely applied to address different problems. The generic microchips containing a complete set of possible hexanucleotides are used to reveal the DNA motifs binding with different proteins and to study the DNA-protein interactions. The oligonucleotide microchips are a cheap and reliable tool of diagnostics designed for mass application. Biochips have been developed for identification of the tuberculosis pathogen and its antibiotic-resistant forms; for diagnostics of orthopoxviruses, including the smallpox virus; for diagnostics of the anthrax pathogen; and for identification of chromosomal rearrangements in leukemia patients. The protein microchips can be adapted for further use in proteomics. Bacterial and yeast cells were also immobilized in the gel, maintaining their viability, which open a wide potential for creation biosensors on the basis of microchips. 相似文献
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74.
G G Shershun O F Kagan A A Kiiashko I N Butvin L M Rubina 《Ukrainski? biokhimicheski? zhurnal》1986,58(3):78-80
The total proteolytic activity in blood and tissues and peroxide resistance of erythrocytes were studied in animals at different stages of burn disease. Carbaton, an antioxidant of a pyridine series, is analyzed for its effect on these indices. Considerable activation of proteolysis in tissues and a decrease of the erythrocyte resistance at burn shock and septicotoxemia stages are established. Carbaton being applied as a drug, influences positively the studied processes; a degree of the shift correction depends on the preparation dose. 相似文献
75.
Jolyon P. Mitchell Mark W. Nagel Cathy C. Doyle Rubina S. Ali Valentina I. Avvakoumova J. David Christopher Jorge Quiroz Helen Strickland Terrence Tougas Svetlana Lyapustina 《AAPS PharmSciTech》2010,11(3):1115-1118
The purpose of this study was to resolve an anomalously high measure of extra-fine particle fraction (EPF) determined by the
abbreviated cascade impactor possibly relevant for human respiratory tract (AIM-HRT) in the experiment described in Part 1
of this two-part series, in which the relative precision of abbreviated impactors was evaluated in comparison with a full
resolution Andersen eight-stage cascade impactor (ACI). Evidence that the surface coating used to mitigate particle bounce
was laterally displaced by the flow emerging from the jets of the lower stage was apparent upon microscopic examination of
the associated collection plate of the AIM-HRT impactor whose cut point size defines EPF. A filter soaked in surfactant was
floated on top of this collection plate, and further measurements were made using the same pressurized metered-dose inhaler-based
formulation and following the same procedure as in Part 1. Measures of EPF, fine particle, and coarse particle fractions were
comparable with those obtained with the ACI, indicating that the cause of the bias had been identified and removed. When working
with abbreviated impactors, this precaution is advised whenever there is evidence that surface coating displacement has occurred,
a task that can be readily accomplished by microscopic inspection of all collection plates after allowing the impactor to
sample ambient air for a few minutes. 相似文献
76.
A. Yu. Rubina M. V. Tsybulskaya O. A. Zasedateleva S. B. Popletaeva M. A. Filippova V. I. Butvilovskaya S. A. Surzhikov A. A. Stomakhin A. S. Zasedatelev 《Molecular Biology》2012,46(4):596-604
The sequence specificity of protein—oligonucleotide interactions based on the example of binase interaction with oligodeoxyribonucleotides immobilized in biochip gel elements has been studied. Constants of the preferable binding of binase to the selected nucleotide sequences were measured. The GAGAGAG and GAGAGAGAG oligodeoxyribonucleotides, which specifically bind to binase, were used as molecular probes to develop affine sorptive media for binase isolation and concentration from diluted water solutions. The volume capacity of affinity sorbents with immobilized oligodeoxyribonucleotides was found to be 2.6 and 2.3 mg of binase per 1 mL of sorbent for GAGAGAG and GAGAGAGAG, respectively. It was shown that, after affinity chromatography and elution from sorptive media, the oligonucleotide specificity of binase corresponds to the specificity of the initial sample. 相似文献
77.
Tabassum R Jaiswal A Chauhan G Dwivedi OP Ghosh S Marwaha RK Tandon N Bharadwaj D 《PloS one》2012,7(4):e33162
Background
Hyperhomocysteinemia is regarded as a risk factor for cardiovascular diseases, diabetes and obesity. Manifestation of these chronic metabolic disorders starts in early life marked by increase in body mass index (BMI). We hypothesized that perturbations in homocysteine metabolism in early life could be a link between childhood obesity and adult metabolic disorders. Thus here we investigated association of common variants from homocysteine metabolism pathway genes with obesity in 3,168 urban Indian children.Methodology/Principal Findings
We genotyped 90 common variants from 18 genes in 1,325 children comprising of 862 normal-weight (NW) and 463 over-weight/obese (OW/OB) children in stage 1. The top signal obtained was replicated in an independent sample set of 1843 children (1,399 NW and 444 OW/OB) in stage 2. Stage 1 association analysis revealed association between seven variants and childhood obesity at P<0.05, but association of only rs2796749 in AMD1 [OR = 1.41, P = 1.5×10-4] remained significant after multiple testing correction. Association of rs2796749 with childhood obesity was validated in stage 2 [OR = 1.28, P = 4.2×10-3] and meta-analysis [OR = 1.35, P = 1.9×10-6]. AMD1 variant rs2796749 was also associated with quantitative measures of adiposity and plasma leptin levels that was also replicated and corroborated in combined analysis.Conclusions/Significance
Our study provides first evidence for the association of AMD1 variant with obesity and plasma leptin levels in children. Further studies to confirm this association, its functional significance and mechanism of action need to be undertaken. 相似文献78.
79.
Sofia Conde Margarida Borrego Tania Teixeira Rubina Teixeira Anabela Sá Paula Soares 《Reports of Practical Oncology and Radiotherapy》2013,18(2):67-75
Aim
To evaluate the prognostic factors and impact on survival of neoadjuvant oral and infusional chemoradiotherapy in patients with locally advanced rectal cancer.Background
There is still no definitive consensus about the prognostic factors and the impact of neoadjuvant chemoradiotherapy on survival. Some studies have pointed to an improvement in overall survival (OS) and progression-free survival (PFS) in patients with tumor downstaging (TD) and nodal downstaging (ND).Materials and methods
A set of 159 patients with LARC were treated preoperatively. Group A – 112 patients underwent concomitant oral chemoradiotherapy: capecitabine or UFT + folinic acid. Group B – 47 patients submitted to concomitant chemoradiation with 5-FU in continuous infusion. 63.6% of patients were submitted to adjuvant chemotherapy.Results
Group A: pathologic complete response (pCR) – 18.7%; TD – 55.1%; ND – 76%; loco-regional response – 74.8%. Group B: pCR – 11.4%; TD – 50%; ND – 55.8%; LRR – 54.5%. The loco-regional control was 95.6%. There was no difference in survival between both groups. Those with loco-regional response had better PFS.Conclusions
Tumor and nodal downstaging, loco-regional response and a normal CEA level turned out to be important prognostic factors in locally advanced rectal cancer. Nodal downstaging and loco-regional response were higher in Group A. Those with tumor downstaging and loco-regional response from Group A had better OS. Adjuvant chemotherapy had no impact on survival except in those patients with loco-regional response who achieved a higher PFS. 相似文献80.
WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition 总被引:1,自引:0,他引:1
Ho H Kapadia R Al-Tahan S Ahmad S Ganesan AK 《The Journal of biological chemistry》2011,286(14):12509-12523