The sesquiterpene botrydial produced by Botrytis cinerea induces the hypersensitive response on plant tissues and its action is modulated by salicylic acid and jasmonic acid signaling

Botrytis cinerea, as a necrotrophic fungus, kills host tissues and feeds on the remains. This fungus is able to induce the hypersensitive response (HR) on its hosts, thus taking advantage on the host's defense machinery for generating necrotic tissues. However, the identity of HR effectors produced by B. cinerea is not clear. The aim of this work was to determine whether botrydial, a phytotoxic sesquiterpene produced by B. cinerea, is able to induce the HR on plant hosts, using Arabidopsis thaliana as a model. Botrydial induced the expression of the HR marker HSR3, callose deposition, and the accumulation of reactive oxygen species and phenolic compounds. Botrydial also induced the expression of PR1 and PDF1.2, two pathogenesis-related proteins involved in defense responses regulated by salicylic acid (SA) and jasmonic acid (JA), respectively. A. thaliana and tobacco plants defective in SA signaling were more resistant to botrydial than wild-type plants, as opposed to A. thaliana plants defective in JA signaling, which were more sensitive. It can be concluded that botrydial induces the HR on its hosts and its effects are modulated by host signaling pathways mediated by SA and JA.     

The impact of temperature on balancing immune responsiveness and growth in Arabidopsis

Plants have evolved polymorphic immune receptors to recognize pathogens causing disease. However, triggering of resistance needs to be tuned to the local environment to maintain a balance between defense and growth. We consider here the impact of temperature as a key environmental factor influencing immune pathway activation in Arabidopsis. Genetic compensatory and molecular buffering mechanisms affecting the diversification, functionality and subcellular dynamics of immune receptors, reveal multiple points at which temperature intersects with host resistance signaling systems, including a role of at least one receptor in sensing temperature change. Analysis of temperature-dependent autoimmunity caused by allelic mismatches in hybrids of evolutionary diverged Arabidopsis accessions is illuminating processes by which plants maintain 'poise' between immune responsiveness and fitness in natural populations.