窄带成像内镜、染色内镜及常规内镜模式诊断结直肠增生性病变的应用价值比较研究

目的:探讨窄带成像内镜(NBI)、染色内镜及常规内镜模式鉴别诊断非肿瘤性、肿瘤性结直肠增生性病变的应用价值。方法:选择2017年2月至2019年3月西安市中心医院消化科收治的结直肠增生性病变患者,均行NBI、染色内镜、常规内镜检查。比较三种模式图像清晰度以及鉴别诊断非肿瘤性、肿瘤性结直肠增生性病变的效能。结果:NBI、染色内镜模式图像质量评分分布优于常规内镜(P<0.05),NBI图像质量评分分布优于染色内镜模式(P<0.05)。以病理结果为准,常规内镜结直肠增生性病变检出率73.13%,NBI 91.04%,染色内镜96.26%,NBI、染色内镜结直肠增生性病变检出率高于常规内镜(P<0.05),NBI、染色内镜比较无统计学差异(P>0.05)。NBI模式下检测NBI分型与病理组织学结果一致性较好(kappa值=0.801,P<0.05)。NBI、染色内镜诊断肿瘤性结直肠增生性病变的灵敏度、特异度、阳性预测值、阴性预测值、准确度均明显高于常规内镜,染色内镜、NBI、常规内镜诊断肿瘤性结直肠增生性病变的曲线下面积(AUC)分别为0.844(95%CI:0.812~0.956)、0.921(95%CI:0.860~0.982)、0.750(95%CI:0.651~0.848)。结论:NBI、染色内镜在鉴别非肿瘤性和肿瘤性结直肠增生性病变方面效能相似,均优于常规内镜,NBI分型与病理组织学结果一致性高,更适合结直肠增生性病变的鉴别诊断。     

Intraclonal and interclonal differences of ploidy in transplantable rat rhabdomyosarcoma RA-2 detected using DNA flow cytometry

DNA contents were determined using flow cytometry in cells of 71 clones of the rat transplantable rhabdomyosarcoma passed through more than 70 cycles of selection for increased malignancy. 58 clones (82%) consisted of cells with diploid DNA content, whereas 13 clones (18%) displayed not only diploid, but also near tetraploid cell subpopulations, the latter covering from 20 to 60% of the total number of cells measured. The data obtained show the existence of intra- and interclonal karyotypic heterogeneity in populations of tumor clonogenic cells.     

The effect of caffeine on the duration of the mitotic phase cycle in CHO-K1 Chinese hamster cells, irradiated with x-rays

Alterations in the duration of mitotic cycle phases in X-irradiated Chinese hamster cells CHO K1 after caffeine (CF) treatment are studied. Delays in S- and G2-phases, induced by 1 and 5 Gr of X-irradiation, are partially or completely decreased by 1 mM or 5 mM CF, respectively. When CF is removed from the medium after irradiation, delays in S- and G2-phases are seen again, however long (0-12 hours) CF remains in the medium. The data obtained allow to suggest that since CF results in a radioresistant DNA synthesis, it may also postpone delays in S- and G2-phases, while cells are progressing through the cell cycle.     

Efficiency of laser energy input into a hohlraum through a hole

Results are presented from the two-dimensional numerical simulations of laser energy input into a hohlraum through a hole. This problem is of interest for ICF research, specifically, for optimization of laser microtarget design. The optimum relations are found between the hole size and the effective laser spot radius under conditions close to those of present-day ICF experiments.     

The interaction of lipoic acid and its metabolites with aspartate and alanine aminotransferases in a multienzyme complex from rat liver and brain mitochondria]

It is shown that lipoic acid and its metabolites--lipoamide and 4,6-dithiohexanoic acid can interact and inhibit the mitochondrial aspartate- and alanine-aminotransferase activities. This effect is much more pronounced in the case of multienzyme complexes--metabolon.     

The parasite-host relationships between the glochidia of the European pearl oyster Margaritifera margaritifera (Margaritiferidae: Bivalvia) and mass fish species in the European north of the USSR

Narrow specificity of larvae (glochidia) of Margaritifera margaritifera to salmon in the rivers of the Kola Peninsula was proved experimentally. It was found that in the gills of minnow, the other mass fish in the northern rivers of the USSR, larvae of M. margaritifera cannot develop and perish. Reasons causing the narrow specificity of M. margaritifera to Salmonidae are discussed.