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81.
Rohan R. Singh Jodie A. Kemp James F. Kollmorgen Javed A. Qureshi Geoffrey B. Fincher 《Plant Cell, Tissue and Organ Culture》1997,49(2):121-127
Fertile plants were regenerated from both cell suspension and protoplast-derived cultures of the two-row barley, Hordeum vulgare
L. cv. Schooner. Embryogenic calluses, derived from immature embryos, were used to establish suspension cultures. More than
100 plants, with variable seed set, have been regenerated from six embryogenic cell suspension cultures. Protoplasts isolated
from three suspension cultures divided and when the resultant embryogenic proto-calluses were transferred to regeneration
medium both green and albino shoots were produced. The green shoots were transferred to growth regulator-free medium and plantlets
that developed strong root systems were potted in soil and grown to maturity in the glasshouse. Root tip analysis of plants
regenerated from cell suspension cultures revealed the expected 2N = 14 complement of chromosomes. However, chromosomal analysis
of protoplast-derived plants showed numerical variation among a proportion of the regenerants.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
82.
Rohan?M.?Brooker Mark?E.?Hay Danielle?L.?DixsonEmail author 《Coral reefs (Online)》2016,35(4):1263-1270
Coral reefs worldwide are shifting from high-diversity, coral-dominated communities to low-diversity systems dominated by seaweeds. This shift can impact essential recovery processes such as larval recruitment and ecosystem resilience. Recent evidence suggests that chemical cues from certain corals attract, and from certain seaweeds suppress, recruitment of juvenile fishes, with loss of coral cover and increases in seaweed cover creating negative feedbacks that prevent reef recovery and sustain seaweed dominance. Unfortunately, the level of seaweed increase and coral decline that creates this chemically cued tipping point remains unknown, depriving managers of data-based targets to prevent damaging feedbacks. We conducted flume and field assays that suggest juvenile fishes sense and respond to cues produced by low levels of seaweed cover. However, the herbivore species we tested was more tolerant of degraded reef cues than non-herbivores, possibly providing some degree of resilience if these fishes recruit, consume macroalgae, and diminish negative cues. 相似文献
83.
Hartmut Jahns Rohan Degaonkar Peter Podbevsek Swati Gupta Anna Bisbe Krishna Aluri John Szeto Pawan Kumar Sarah LeBlanc Tim Racie Christopher
R Brown Adam Castoreno Dale
C Guenther Vasant Jadhav Martin
A Maier Janez Plavec Martin Egli Muthiah Manoharan Ivan Zlatev 《Nucleic acids research》2021,49(18):10250
84.
85.
A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis 下载免费PDF全文
Hugo Valdez Juan Martín Laborde Fabricio Maschi Miguel Ayala Rohan Shah Marcelo Fernandez Lahore Maria Eugenia Rodriguez 《Microbiology and immunology》2017,61(10):407-415
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87.
Fink JL Karunaratne S Mittal A Gardiner DM Hamilton N Mahony D Kai C Suzuki H Hayashizaki Y Teasdale RD 《Genome biology》2008,9(1):R15-8
Background
The nucleus is a complex cellular organelle and accurately defining its protein content is essential before any systematic characterization can be considered.Results
We report direct evidence for 2,568 mammalian proteins within the nuclear proteome: the nuclear subcellular localization of 1,529 proteins based on a high-throughput subcellular localization protocol of full-length proteins and an additional 1,039 proteins for which clear experimental evidence is documented in published literature. This is direct evidence that the nuclear proteome consists of at least 14% of the entire proteome. This dataset was used to evaluate computational approaches designed to identify additional nuclear proteins.Conclusion
This represents direct experimental evidence that the nuclear proteome consists of at least 14% of the entire proteome. This high-quality nuclear proteome dataset was used to evaluate computational approaches designed to identify additional nuclear proteins. Based on this analysis, researchers can determine the stringency and types of lines of evidence they consider to infer the size and complement of the nuclear proteome. 相似文献88.
Liviu R Totir Rohan L Fernando Jack CM Dekkers Soledad A Fernández Bernt Guldbrandtsen 《遗传、选种与进化》2003,35(7):585-604
An increased availability of genotypes at marker loci has prompted the development of models that include the effect of individual genes. Selection based on these models is known as marker-assisted selection (MAS). MAS is known to be efficient especially for traits that have low heritability and non-additive gene action. BLUP methodology under non-additive gene action is not feasible for large inbred or crossbred pedigrees. It is easy to incorporate non-additive gene action in a finite locus model. Under such a model, the unobservable genotypic values can be predicted using the conditional mean of the genotypic values given the data. To compute this conditional mean, conditional genotype probabilities must be computed. In this study these probabilities were computed using iterative peeling, and three Markov chain Monte Carlo (MCMC) methods – scalar Gibbs, blocking Gibbs, and a sampler that combines the Elston Stewart algorithm with iterative peeling (ESIP). The performance of these four methods was assessed using simulated data. For pedigrees with loops, iterative peeling fails to provide accurate genotype probability estimates for some pedigree members. Also, computing time is exponentially related to the number of loci in the model. For MCMC methods, a linear relationship can be maintained by sampling genotypes one locus at a time. Out of the three MCMC methods considered, ESIP, performed the best while scalar Gibbs performed the worst. 相似文献
89.
Nanofactories are nano-dimensioned and comprised of modules serving various functions that alter the response of targeted cells when deployed by locally synthesizing and delivering cargo to the surfaces of the targeted cells. In its basic form, a nanofactory consists of a minimum of two functional modules: a cell capture module and a synthesis module. In this work, magnetic nanofactories that alter the response of targeted bacteria by the localized synthesis and delivery of the "universal" bacterial quorum sensing signal molecule autoinducer AI-2 are demonstrated. The magnetic nanofactories consist of a cell capture module (chitosan-mag nanoparticles) and an AI-2 biosynthesis module that contains both AI-2 biosynthetic enzymes Pfs and LuxS on a fusion protein (His-LuxS-Pfs-Tyr, HLPT) assembled together. HLPT is hypothesized to be more efficient than its constituent enzymes (used separately) at conversion of the substrate SAH to product AI-2 on account of the proximity of the two enzymes within the fusion protein. HLPT is demonstrated to be more active than the constituent enzymes, Pfs and LuxS, over a wide range of experimental conditions. The magnetic nanofactories (containing bound HLPT) are also demonstrated to be more active than free, unbound HLPT. They are also shown to elicit an increased response in targeted Escherichia coli cells, due to the localized synthesis and delivery of AI-2, when compared to the response produced by the addition of AI-2 directly to the cells. Studies investigating the universality of AI-2 and unraveling AI-2 based quorum sensing in bacteria using magnetic nanofactories are envisioned. The prospects of using such multi-modular nanofactories in developing the next generation of antimicrobials based on intercepting and interrupting quorum sensing based signaling are discussed. 相似文献
90.