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991.
Isolation and in vitro expansion of human colonic stem cells 总被引:1,自引:0,他引:1
Jung P Sato T Merlos-Suárez A Barriga FM Iglesias M Rossell D Auer H Gallardo M Blasco MA Sancho E Clevers H Batlle E 《Nature medicine》2011,17(10):1225-1227
Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population. 相似文献
992.
Brito-Echeverría J Lucio M López-López A Antón J Schmitt-Kopplin P Rosselló-Móra R 《Extremophiles : life under extreme conditions》2011,15(3):379-389
We have studied the response of the two closest relative strains M8 and M31 of Salinibacter ruber to environmental changes as the transition from exponential to stationary phase in a batch growth, and the submission to
two different environmental stresses (dilution of the culture medium and temperature decrease). We monitored the changes in
cultivability, ribosomal content by fluorescence in situ hybridization (FISH), and metabolic changes with high-field ion cyclotron
Fourier transform mass spectrometry. In all cases, we could observe an important decrease in cultivability that was not accompanied
by a decrease in FISH counts, pointing to a transition to viable but non-cultivable state rather than cell death. Furthermore,
the metabolomic analyses indicated a common response of both strains to the different conditions assayed. Only a small portion
of the detected masses could be annotated due to database constraints. Among them, the most remarkable changes could be attributed
to modifications in the composition of the cell envelope, and especially in the cell membrane. We could track changes in the
length or saturation of the fatty acids and in the composition of phospholipids involved in aminosugar, glycerolipid, and
glycerophospholipid metabolic pathways. 相似文献
993.
994.
Maravilla P Garza-Rodriguez A Gomez-Diaz B Jimenez-Gonzalez DE Toral-Bastida E Martinez-Ocaña J West B Molina N Garcia-Cortes R Kawa-Karasik S Romero-Valdovinos M Avila-Ramirez G Flisser A 《Parasitology international》2011,60(4):364-370
Chinchilla laniger has been reported as an experimental definitive host for Taenia solium; however no information about its suitability and yield of gravid tapeworm proglottids containing viable and infective eggs has been published. In total 55 outbred female chinchillas were infected with 4 cysticerci each; hosts were immunodeppressed with 6 or 8 mg of methyl-prednisolone acetate every 14 days starting the day of infection and their discomfort was followed. Kinetics of coproantigen ELISA or expelled proglottids was used to define the infection status. Efficiency of tapeworm establishment was 21% and of parasite gravidity was 8%; chinchillas showed some degree of suffering along the infection. Viability of eggs obtained from gravid proglottids was tested comparing methods previously published, our results showed 62% viability with propidium iodide, 54% with trypan blue, 34% with neutral red, 30% by oncosphere activation and 7% with bromide 3-(4,5-dimetil-tiazol-2-il)-2,5-difenil-tetrazolio (MTT) reduction; no statistical differences were obtained between most techniques, except activation. Four piglets were infected with 50,000 eggs each, necropsy was performed 3 months later and, after counting the number of cysticerci recovered, the percentage of infection was similar to data obtained with T. solium eggs recovered from humans. Our results demonstrate that the experimental model of T. solium taeniasis in C. laniger is a good alternative for providing eggs and adult tapeworms to be used in different types of experiments; optimization of the model probably depends on the use of inbred hosts and on the reduction of infected animals' suffering. 相似文献
995.
Suárez-Suárez A López-López A Tovar-Sánchez A Yarza P Orfila A Terrados J Arnds J Marqués S Niemann H Schmitt-Kopplin P Amann R Rosselló-Móra R 《Environmental microbiology》2011,13(6):1488-1499
In situ mesocosm experiments using a calcareous sand flat from a coastal area of the island of Mallorca in the Mediterranean Sea were performed in order to study the response of sulfate-reducing bacteria (SRB) to controlled crude oil contamination, or heavy contamination with naphthalene. Changes in the microbial community caused by the contamination were monitored by a combination of comparative sequence analysis of 16S rRNA genes, fluorescence in situ hybridization, cultivation approaches and metabolic activity rates. Our results showed that crude oil and naphthalene negatively influenced the total microbial community as the natural increase in cell numbers due to the seasonal dynamics was attenuated. However, both contaminants enhanced the sulfate reduction rates, as well as the culturability of SRB. Our results suggested the presence of autochthonous deltaproteobacterial SRBs that were able to degrade crude oil or polycyclic aromatic hydrocarbons such as naphthalene in anaerobic sediment layers. 相似文献
996.
Madhuresh K. Choudhary Jong Moon Yoon Ramon Gonzalez Jacqueline V. Shanks 《Biotechnology and Bioprocess Engineering》2011,16(3):419-437
Improved design of metabolic flux estimation using mixed label 13C labeling experiments and identifiability analysis motivated re-examination of metabolic fluxes during anaerobic fermentation
in the Escherichia coli. Comprehensive metabolic flux maps were determined by using a mixture of differently labeled glucose and compared to conventional
flux maps obtained using extracellular measurements and comprehensive metabolic flux maps obtained using only U-13C glucose as the substrate. As expected, conventional flux analysis performs poorly in comparison to 13C-MFA, especially in the Embden-Meyerhof-Parnas (EMP) and pentose phosphate (PP) pathways. Identifiability analysis indicated
and experiments confirmed that a mixture of 10% U-l3C glucose, 25% 1-13C glucose, and 65% naturally labeled glucose significantly improved the statistical quality of all calculated fluxes in the
PP pathway, the EMP pathway, the anaplerotic reactions, and the tricarboxylic acid cycle. Modifying the network topology for
the presence and absence of the Entner-Doudoroff pathway and the glyoxylate shunt did not affect the value or quality of estimated
fluxes significantly. Extracellular measurement of formate production was necessary for the accurate estimation of the fluxes
around the formate node. 相似文献
997.
Wijsman EM Pankratz ND Choi Y Rothstein JH Faber KM Cheng R Lee JH Bird TD Bennett DA Diaz-Arrastia R Goate AM Farlow M Ghetti B Sweet RA Foroud TM Mayeux R;NIA-LOAD/NCRAD Family Study Group 《PLoS genetics》2011,7(2):e1001308
Late-onset Alzheimer''s disease (LOAD) is the most common form of dementia in the elderly. The National Institute of Aging-Late Onset Alzheimer''s Disease Family Study and the National Cell Repository for Alzheimer''s Disease conducted a joint genome-wide association study (GWAS) of multiplex LOAD families (3,839 affected and unaffected individuals from 992 families plus additional unrelated neurologically evaluated normal subjects) using the 610 IlluminaQuad panel. This cohort represents the largest family-based GWAS of LOAD to date, with analyses limited here to the European-American subjects. SNPs near APOE gave highly significant results (e.g., rs2075650, p = 3.2×10−81), but no other genome-wide significant evidence for association was obtained in the full sample. Analyses that stratified on APOE genotypes identified SNPs on chromosome 10p14 in CUGBP2 with genome-wide significant evidence for association within APOE ε4 homozygotes (e.g., rs201119, p = 1.5×10−8). Association in this gene was replicated in an independent sample consisting of three cohorts. There was evidence of association for recently-reported LOAD risk loci, including BIN1 (rs7561528, p = 0.009 with, and p = 0.03 without, APOE adjustment) and CLU (rs11136000, p = 0.023 with, and p = 0.008 without, APOE adjustment), with weaker support for CR1. However, our results provide strong evidence that association with PICALM (rs3851179, p = 0.69 with, and p = 0.039 without, APOE adjustment) and EXOC3L2 is affected by correlation with APOE, and thus may represent spurious association. Our results indicate that genetic structure coupled with ascertainment bias resulting from the strong APOE association affect genome-wide results and interpretation of some recently reported associations. We show that a locus such as APOE, with large effects and strong association with disease, can lead to samples that require appropriate adjustment for this locus to avoid both false positive and false negative evidence of association. We suggest that similar adjustments may also be needed for many other large multi-site studies. 相似文献
998.
999.
Shafinaz F. Chowdhury Ramon Hurtado Guerrero Reto Brun Luis M. Ruiz-Perez Dolores Gonzalez Pacanowska Ian H. Gilbert 《Journal of enzyme inhibition and medicinal chemistry》2013,28(5):293-302
Dihydrofolate reductase is a drug target that has not been thoroughly investigated in leishmania and trypanosomes. Work has previously shown that 5-benzyl-2,4-diaminopyrimidines are selective inhibitors of the leishmanial and trypanosome enzymes. Modelling predicted that alkyl/aryl substitution on the 6-position of the pyrimidine ring should increase enzyme activity of 5-benzyl-2,4-diaminopyrimidines as inhibitors of leishmanial and trypanosomal dihydrofolate reductase. Various compounds were prepared and evaluated against both the recombinant enzymes and the intact organisms. The presence of a substituent had a small or negative effect on activity against the enzyme or intact parasites compared to unsubstituted compounds. 相似文献
1000.
Vera Hemleben Ales Kovarik Ramon A. Torres‐Ruiz Roman A. Volkov Thengiz Beridze 《分类学与生物多样性》2013,11(3):277-289
Relationships among genomes are often revealed by the occurrence of common or related satellite DNA (satDNA) types. A typical satDNA characterized by specific sites for one (or more) restriction endonuclease(s) is called ‘restriction satellite DNA’. Restriction satDNA comprises ‐ in addition to transposons and retrotransposable elements ‐ often highly repeated genome components present in most higher plants. Large arrays of satDNA elements are concentrated at subtelo‐meric and/or centromeric regions (intermingled with other retrotransposon‐derived elements), however, they can be also located as large intercalating blocks along the chromosome. The head‐to‐tail tandemly arranged repeat units (monomers) of satDNA mostly exhibit lengths of 160 to 180 bp or 320 to 370 bp, but other lengths were also found in plants. In particular, in interspecific hybrids between more distantly related species, which often exist only after polyploidization, the individual repetitive DNA of the crossing partners contribute to recombination and rearrangement processes in the hybrids, thereby stimulating genome evolution. Here, we concentrate on the possible origin, molecular evolution, organization and distribution of highly repeated satDNA in various higher plants with emphasis on hybrids and allopolyploids. 相似文献