全文获取类型
收费全文 | 381287篇 |
免费 | 40732篇 |
国内免费 | 324篇 |
专业分类
422343篇 |
出版年
2018年 | 3611篇 |
2017年 | 3443篇 |
2016年 | 5005篇 |
2015年 | 7083篇 |
2014年 | 8160篇 |
2013年 | 11252篇 |
2012年 | 13056篇 |
2011年 | 12829篇 |
2010年 | 8590篇 |
2009年 | 7568篇 |
2008年 | 11240篇 |
2007年 | 11711篇 |
2006年 | 10983篇 |
2005年 | 10550篇 |
2004年 | 10197篇 |
2003年 | 9792篇 |
2002年 | 9766篇 |
2001年 | 19281篇 |
2000年 | 19619篇 |
1999年 | 15114篇 |
1998年 | 4677篇 |
1997年 | 4873篇 |
1996年 | 4656篇 |
1995年 | 4330篇 |
1994年 | 4288篇 |
1993年 | 4105篇 |
1992年 | 12111篇 |
1991年 | 11737篇 |
1990年 | 11284篇 |
1989年 | 10808篇 |
1988年 | 9998篇 |
1987年 | 9321篇 |
1986年 | 8528篇 |
1985年 | 8379篇 |
1984年 | 6790篇 |
1983年 | 5929篇 |
1982年 | 4448篇 |
1981年 | 3922篇 |
1980年 | 3611篇 |
1979年 | 6475篇 |
1978年 | 4871篇 |
1977年 | 4383篇 |
1976年 | 3983篇 |
1975年 | 4640篇 |
1974年 | 4806篇 |
1973年 | 4686篇 |
1972年 | 4370篇 |
1971年 | 3779篇 |
1970年 | 3406篇 |
1969年 | 3198篇 |
排序方式: 共有10000条查询结果,搜索用时 8 毫秒
991.
992.
Summary Mature spinach plants were held in the dark for several days. The photochemical activities and the activity of some enzymes related to NADP reduction were follwed in the chloroplasts isolated from leaves after dark starvation. Photosystem-II, measured by reduction of DPIP, remained stable during 6 days of darkening. The decrease of NADP reduction which appeared after 2 days of starvation was found to be due to protein autolysis rather than inactivation of the photosystems. The stability of photosystem-I was demonstrated by reactivation of NADP reduction after addition of purified ferredoxin and ferredoxin-NADP-reductase. After 4 days of starvation the restoration of the NADP reduction required in addition another, low-molecular-weight factor. From the isolation procedure and from its properties this factor is assumed to be identical with FRS. However, even in the presence of FRS only half of the total activity is restored after 7 days. The activity of the NADP-reducing system is restored in vivo when plants kept for 7 days in the dark are again illuminated.Abbreviations NADP
nicotinamide-adenine-dinucleotide phosphate
- DPIP
2,6-dichlorophenolindophenol
- DCMU
(3,4-dichlorophenyl)-1,1-dimethylurea
- FRS
ferredoxin-reducing-substance 相似文献
993.
Brain and body weights, contents of DNA and protein and activities of 1,6-diphosphofructoaldolase (aldolase, EC 4.1.2.13), creatine phosphokinase (CPK, EC 2.7.3.2), and isocitric dehydrogenase (ICD, EC 1.1.1.42) in brain (minus cerebellum and brain stem) were studied in control and food-deprived rats at 7, 14 and 21 days of postnatal age. Activities of all three enzymes per brain were less in the food-deprived animals. In both groups of rats the ratios of aldolase/DNA and CPK/DNA increased with maturation, indicating that increasing activity per brain during maturation was the result of both increased activity per cell and increased numbers of cells. The ratio of ICD/DNA decreased with maturation but was essentially the same in both the food-deprived and control groups. Increase of ICD activity per brain with maturation was attributable to increased numbers of cells. Food deprivation in immature animals resulted in lowered activities per brain for aldolase, CPK and ICD because of diminished cell multiplication. 相似文献
994.
Avian oviductal fluids contain phosphorylating-dephosphorylating enzymes that might function in sperm-oocyte interactions. Phosphorprotein phosphatase and protein kinase have been purified 20-fold and 40-fold, respectively. The latter easily aggregates and is highly labile. Other properties are comparable to those of holoenzymes. 相似文献
995.
996.
A 14 kDa polypeptide in rat ileal cytosol has been identified as the major intestinal cytosolic bile acid-binding protein (I-BABP) by photoaffinity labeling with the radiolabeled 7,7-azo derivative of taurocholate (7,7-azo-TC). To further characterize I-BABP, the protein was purified by lysylglycocholate Sepharose 4B affinity and DE-52 anion-exchange chromatography. The purified I-BABP contained a single 14 kDa band on SDS-PAGE. The 14 kDa protein showed a 26-fold increase in binding affinity for [3H]7,7-azo-TC compared to cytosolic protein. Immunoblotting of protein fractions separated by affinity chromatography showed that neither liver fatty acid binding protein (L-FABP) nor intestinal fatty acid binding protein (I-FABP) bind to the affinity column and that the 14 kDa protein which bound to the column and was subsequently eluted with detergent did not cross-react with anti-L-FABP or anti-I-FABP. The 14 kDa protein labeled with [3H]7,7-azo-TC was radioimmunoprecipitated from cytosol by rabbit antiserum raised against purified I-BABP. I-BABP was shown to have a blocked N-terminus; however, its mixed internal sequence generated from cyanogen bromide-cleaved protein and amino acid composition indicated that it was related to (although clearly distinct from) both I-FABP and L-FABP. These studies have isolated a 14 kDa bile acid-binding protein from rat ileal cytosol which is immunologically and biochemically distinct from I-FABP and L-FABP. 相似文献
997.
K Watanabe Y Fujii H Ohkubo S Kuramitsu H Kagamiyama S Nakanishi O Hayaishi 《Biochemical and biophysical research communications》1991,181(1):272-278
The full-length bovine lung prostaglandin(PG) F synthase cDNA was constructed from partial cDNA clones and ligated into bacterial expression vector pUC8 to develop expression plasmid pUCPF1. This plasmid permitted the synthesis of bovine lung PGF synthase in Escherichia coli. The recombinant bacteria overproduced a 36-KDa protein that was recognized by anti-PGF synthase antibody, and the expressed protein was purified to apparent homogeneity. The expressed protein reduced not only carbonyl compounds including PGD2 and phenanthrenequinone but also PGH2; and the Km values for phenanthrenequinone, PGD2, and PGH2 of the expressed protein were 0.1, 100, and 8 microM, respectively, which are the same as those of the bovine lung PGF synthase. The protein produced PGF2 alpha from PGH2, and 9 alpha, 11 beta-PGF2 from PGD2 at different active sites. Moreover, the structure of the purified protein from Escherichia coli was essentially identical to that of the native enzyme in terms of C-terminal sequence, sulfhydryl groups, and CD spectra except that the nine amino acids provided by the lac Z' gene of the vector were fused to the N-terminus. These results indicate that the expressed protein is essentially identical to bovine lung PGF synthase. We confirmed that PGF synthase is a dual function enzyme catalyzing the reduction of PGH2 and PGD2 on a single enzyme and that it has one binding site for NADPH. 相似文献
998.
R K Sharma 《Biochemical and biophysical research communications》1991,181(1):493-497
Polyclonal antibodies raised against bovine heart high molecular weight calmodulin-binding protein were used to study the distribution of this protein in diverse bovine tissues. The high molecular weight calmodulin-binding protein, in addition to bovine heart, is also present in lung and brain at much lower levels, but not in skeletal muscle, spleen, kidney or uterus. 相似文献
999.
1000.