Biofilm form and function: carbon availability affects biofilm architecture, metabolic activity and planktonic cell yield

Aims: To investigate carbon transformation by biofilms and changes in biofilm architecture, metabolic activity and planktonic cell yield in response to fluctuating carbon availability. Methods and Results: Pseudomonas sp. biofilms were cultured under alternating carbon‐replete and carbon‐limited conditions. A shift to medium without added carbon led to a 90% decrease in biofilm respiration rate and a 40% reduction in planktonic cell yield within 1 h. Attached cell division and progeny release were shown to contribute to planktonic cell numbers during carbon limitation. Development of a significantly enlarged biofilm surface area during carbon limitation facilitated a rapid increase in whole‐biofilm metabolic activity, cell yield and biomass upon the re‐introduction of carbon after 8 days of limitation. The cumulative number of planktonic cells (>10¹⁰ CFU) released from the biofilm during the cultivation period contained only 1·0% of the total carbon available to the biofilm, with 6·5% of the carbon retained in the biofilm and 54% mineralized to CO₂. Conclusions: Biofilm‐derived planktonic cell yield is a proliferation mechanism. The rapid response of biofilms to environmental perturbations facilitates the optimal utilization of resources to promote both proliferation and survival. Biofilms function as efficient catalysts for environmental carbon transformation and mineralization. Significance and Impact of the study: A greater understanding of the relationship between biofilm form and function can inform strategies intended to control and/or promote biofilm formation.     

Anti-transforming growth factor ß antibody treatment rescues bone loss and prevents breast cancer metastasis to bone

Breast cancer often metastasizes to bone causing osteolytic bone resorption which releases active TGFβ. Because TGFβ favors progression of breast cancer metastasis to bone, we hypothesized that treatment using anti-TGFβ antibody may reduce tumor burden and rescue tumor-associated bone loss in metastatic breast cancer. In this study we have tested the efficacy of an anti-TGFβ antibody 1D11 preventing breast cancer bone metastasis. We have used two preclinical breast cancer bone metastasis models, in which either human breast cancer cells or murine mammary tumor cells were injected in host mice via left cardiac ventricle. Using several in vivo, in vitro and ex vivo assays, we have demonstrated that anti-TGFβ antibody treatment have significantly reduced tumor burden in the bone along with a statistically significant threefold reduction in osteolytic lesion number and tenfold reduction in osteolytic lesion area. A decrease in osteoclast numbers (p?=?0.027) in vivo and osteoclastogenesis ex vivo were also observed. Most importantly, in tumor-bearing mice, anti-TGFβ treatment resulted in a twofold increase in bone volume (p<0.01). In addition, treatment with anti-TGFβ antibody increased the mineral-to-collagen ratio in vivo, a reflection of improved tissue level properties. Moreover, anti-TGFβ antibody directly increased mineralized matrix formation in calverial osteoblast (p?=?0.005), suggesting a direct beneficial role of anti-TGFβ antibody treatment on osteoblasts. Data presented here demonstrate that anti-TGFβ treatment may offer a novel therapeutic option for tumor-induced bone disease and has the dual potential for simultaneously decreasing tumor burden and rescue bone loss in breast cancer to bone metastases. This approach of intervention has the potential to reduce skeletal related events (SREs) in breast cancer survivors.     

Anti-Transforming Growth Factor ? Antibody Treatment Rescues Bone Loss and Prevents Breast Cancer Metastasis to Bone

Breast cancer often metastasizes to bone causing osteolytic bone resorption which releases active TGFβ. Because TGFβ favors progression of breast cancer metastasis to bone, we hypothesized that treatment using anti-TGFβ antibody may reduce tumor burden and rescue tumor-associated bone loss in metastatic breast cancer. In this study we have tested the efficacy of an anti-TGFβ antibody 1D11 preventing breast cancer bone metastasis. We have used two preclinical breast cancer bone metastasis models, in which either human breast cancer cells or murine mammary tumor cells were injected in host mice via left cardiac ventricle. Using several in vivo, in vitro and ex vivo assays, we have demonstrated that anti-TGFβ antibody treatment have significantly reduced tumor burden in the bone along with a statistically significant threefold reduction in osteolytic lesion number and tenfold reduction in osteolytic lesion area. A decrease in osteoclast numbers (p = 0.027) in vivo and osteoclastogenesis ex vivo were also observed. Most importantly, in tumor-bearing mice, anti-TGFβ treatment resulted in a twofold increase in bone volume (p<0.01). In addition, treatment with anti-TGFβ antibody increased the mineral-to-collagen ratio in vivo, a reflection of improved tissue level properties. Moreover, anti-TGFβ antibody directly increased mineralized matrix formation in calverial osteoblast (p = 0.005), suggesting a direct beneficial role of anti-TGFβ antibody treatment on osteoblasts. Data presented here demonstrate that anti-TGFβ treatment may offer a novel therapeutic option for tumor-induced bone disease and has the dual potential for simultaneously decreasing tumor burden and rescue bone loss in breast cancer to bone metastases. This approach of intervention has the potential to reduce skeletal related events (SREs) in breast cancer survivors.     

Herpes simplex virus tegument protein VP22 contains an internal VP16 interaction domain and a C-terminal domain that are both required for VP22 assembly into the virus particle

Many steps along the herpesvirus assembly and maturation pathway remain unclear. In particular, the acquisition of the virus tegument is a poorly understood process, and the molecular interactions involved in tegument assembly have not yet been defined. Previously we have shown that the two major herpes simplex virus tegument proteins VP22 and VP16 are able to interact, although the relevance of this to virus assembly is not clear. Here we have constructed a number of recombinant viruses expressing N- and C-terminal truncations of VP22 and have used them to identify regions of the protein involved in its assembly into the virus structure. Analysis of the packaging of these VP22 variants into extracellular virions revealed that the C terminus of VP22 is absolutely required for this process, with removal of the C-terminal 89 residues abrogating its incorporation. However, while these 89 residues alone were sufficient for specific incorporation of small amounts of VP22 into the tegument, efficient packaging of VP22 to the levels of full-length protein required an additional 52 residues of the protein. Coimmunoprecipitation assays indicated that these 52 residues also contained the interaction domain for VP16. Furthermore, analysis of the subcellular localization of the mutant forms of VP22 revealed that only those truncations that were efficiently assembled formed characteristic cytoplasmic trafficking complexes, suggesting that these complexes may represent the cellular location for VP22 assembly into the virus. Taken together, these results suggest that there are two determinants involved in the packaging of VP22-a C-terminal domain and an internal VP16 interaction domain, both of which are required for the efficient recruitment of VP22 to sites of virus assembly.     

Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate

Ubiquitin C-terminal hydrolases (UCHs) comprise a family of small ubiquitin-specific proteases of uncertain function. Although no cellular substrates have been identified for UCHs, their highly tissue-specific expression patterns and the association of UCH-L1 mutations with human disease strongly suggest a critical role. The structure of the yeast UCH Yuh1-ubiquitin aldehyde complex identified an active site crossover loop predicted to limit the size of suitable substrates. We report the 1.45 A resolution crystal structure of human UCH-L3 in complex with the inhibitor ubiquitin vinylmethylester, an inhibitor that forms a covalent adduct with the active site cysteine of ubiquitin-specific proteases. This structure confirms the predicted mechanism of the inhibitor and allows the direct comparison of a UCH family enzyme in the free and ligand-bound state. We also show the efficient hydrolysis by human UCH-L3 of a 13-residue peptide in isopeptide linkage with ubiquitin, consistent with considerable flexibility in UCH substrate size. We propose a model for the catalytic cycle of UCH family members which accounts for the hydrolysis of larger ubiquitin conjugates.     

Cutting edge: microbial products elicit formation of dendritic cell aggresome-like induced structures in macrophages

In response to a maturation stimulus, dendritic cells undergo the formation of ubiquitinated protein aggregates known as dendritic cell aggresome-like induced structures (DALIS). DALIS are thought to act as Ag storage structures, allowing for the prioritized degradation of proteins during infection. In this study, we demonstrate that murine macrophages can also form ubiquitinated protein aggregates that are indistinguishable from DALIS. These were formed in a dose- and time-dependent manner, and in response to a variety of microbial products. Surprisingly, the proteasome did not accumulate on these ubiquitinated protein structures, further underlining the difference between DALIS and aggresomes. Our studies suggest that DALIS formation is important for the function of Ag-presenting immune cells during infection.     

Intraspecific differences in the diet of Antarctic fur seals at Nyrøysa, Bouvetøya

Intraspecific differences in the diets of many species of pinnipeds are to be expected in view of the great differences in morphology, life history and foraging behaviour between the sexes of many species. We examined the diet of the Antarctic fur seal population at Bouvetøya, Southern Ocean, to assess intersexual differences. This was made possible by the analysis of prey remains extracted from scats and regurgitations collected in areas used primarily by one or the other sex. The results indicate that both males and females feed primarily on Antarctic krill Euphausia superba with several species of fish and squid being taken, likely opportunistically given their prevalence. Significant differences were identified in the frequency of occurrence of otoliths in scats and the percentage numerical abundance of the major fish prey species in the diet. Adult males ate a smaller quantity of fish overall, but ate significantly more of the larger fish species. The greater diving capabilities of males and the fact that they are not limited in the extent of their foraging area by having to return regularly to feed dependant offspring may play a role in the differences found between the diets of males and females. Additionally, females might be more selective, favouring myctophids because they are richer in energy than krill. The absence of major differences in the diet between the sexes at this location is likely due to the high overall abundance of prey at Bouvetøya.     

SpoIID-Mediated Peptidoglycan Degradation Is Required throughout Engulfment during Bacillus subtilis Sporulation

SpoIID is a membrane-anchored enzyme that degrades peptidoglycan and is essential for engulfment and sporulation in Bacillus subtilis. SpoIID is targeted to the sporulation septum, where it interacts with two other proteins required for engulfment: SpoIIP and SpoIIM. We changed conserved amino acids in SpoIID to alanine to determine whether there was a correlation between the effect of each substitution on the in vivo and in vitro activities of SpoIID. We identified one amino acid substitution, E88A, that eliminated peptidoglycan degradation activity and one, D210A, that reduced it, as well as two substitutions that destabilized the protein in B. subtilis (R106A and K203A). Using these mutants, we show that the peptidoglycan degradation activity of SpoIID is required for the first step of engulfment (septal thinning), as well as throughout membrane migration, and we show that SpoIID levels are substantially above the minimum required for engulfment. The inactive mutant E88A shows increased septal localization compared to the wild type, suggesting that the degradation cycle of the SpoIID/SpoIIP complex is accompanied by the activity-dependent release of SpoIID from the complex and subsequent rebinding. This mutant is also capable of moving SpoIIP across the sporulation septum, suggesting that SpoIID binding, but not peptidoglycan degradation activity, is needed for relocalization of SpoIIP. Finally, the mutant with reduced activity (D210A) causes uneven engulfment and time-lapse microscopy indicates that the fastest-moving membrane arm has greater concentrations of SpoIIP than the slower-moving arm, demonstrating a correlation between SpoIIP protein levels and the rate of membrane migration.Endospore formation is an evolutionarily conserved process that allows Bacillus subtilis and related Gram-positive bacteria to adapt to changes in the environment, such as nutrient depletion. Many dramatic morphological changes occur during sporulation, each requiring a multitude of specialized proteins (reviewed in references 13 and 17). First, a sporulation septum is formed near one of the cell poles, forming two separate compartments of unequal sizes and with differing fates (Fig. ​(Fig.1A).1A). The smaller of the two, the forespore, will eventually become the spore, while the larger, the mother cell, will ultimately lyse. Next, the mother cell membranes move up and around the forespore in the poorly understood process of engulfment. Although this process is superficially similar to eukaryotic engulfment, it is complicated by the thick cell wall that surrounds and separates the two compartments. After engulfment, the migrating membranes pinch off from the mother cell membrane, thereby releasing the forespore into the cytoplasm of the mother cell, where it can be enveloped with protective coat proteins and eventually released into the environment as a mature spore. Sporulation provides an ideal, nonessential system for understanding how bacterial cells are capable of undergoing dramatic morphological changes.Open in a separate windowFIG. 1.Engulfment in B. subtilis. (A) (i) Engulfment begins with formation of an asymmetric septum that divides the cell into the forespore (FS) and mother cell (MC). SpoIID (orange pacman) and SpoIIP (green pacman) peptidoglycan degradation enzymes localize to the center of the septum. (ii) SpoIID and SpoIIP thin the septal peptidoglycan, starting from the center and moving toward the cell edges. SpoIIQ (purple ball) and SpoIIIAH (red ball) form a zipper across the septum, assembling foci behind the leading edges. (iii) The peptidoglycan degradation enzymes localize to the leading edges during membrane migration, while additional SpoIIQ-SpoIIIAH complexes assemble around the forespore. (iv) Engulfment membrane fission occurs at the top of the forespore, releasing the forespore into the mother cell cytoplasm. (B) Burnt-bridge Brownian ratchet model for membrane migration, adapted from earlier studies (1, 7). (C) Schematic representation of the SpoIID domain structure. The transmembrane domain (TM) and putative enzymatic domain, as defined by Pfam (14), are indicated. Amino acid numbers are below the schematic, and mutations causing in vivo phenotypes are indicated by an “X”.Engulfment involves dynamic protein localization and large-scale rearrangements of cellular membranes and peptidoglycan to accommodate internalization of the forespore. The physical basis for engulfment remains unclear, but two separate protein machineries that contribute to engulfment have been discovered. The first module involves the only three proteins known to be required for engulfment under all physiological conditions: SpoIID, SpoIIM, and SpoIIP (16, 24, 35). Zymography assays have demonstrated that both SpoIID and SpoIIP degrade peptidoglycan in vitro (1, 8), and this function is thought to be essential for engulfment in wild-type cells (1, 2, 8). SpoIID and SpoIIP are membrane-spanning proteins that directly interact both in vivo and in vitro, as demonstrated by coimmunoprecipitation and affinity chromatography techniques (2, 8). These studies failed to demonstrate an interaction between SpoIIM and either SpoIID or SpoIIP, perhaps because SpoIIM is an integral membrane protein. However, SpoIIM is required for localization of SpoIID and SpoIIP (2, 8), suggesting that all three proteins interact to form a peptidoglycan degradation module that is essential for engulfment.The second system influencing membrane migration is the SpoIIQ/SpoIIIAH zipper, which is required for engulfment only under certain conditions (2, 7, 38). SpoIIQ is produced in the forespore (23) and SpoIIIAH is produced in the mother cell (19). SpoIIQ and SpoIIIAH interact both in vitro and in vivo via their extracellular domains (6, 7, 10). Because these two proteins are produced in separate compartments, the only possible place for an interaction is the intermembrane space between the mother cell and forespore, forming a protein-protein zipper between the two cells. This zipperlike interaction is necessary for septal localization of SpoIIIAH and other mother cell proteins (6, 10, 18) and is capable of holding the two cells together when peptidoglycan is removed with lysozyme (7). Surprisingly, digestion of the peptidoglycan with lysozyme also allows membrane migration in about half of treated cells, in a process requiring the SpoIIQ/SpoIIIAH zipper but not the SpoIIDMP peptidoglycan degradation module. The SpoIIQ-SpoIIIAH zipper also contributes to engulfment in living cells, since strains lacking SpoIIQ or SpoIIIAH complete engulfment more slowly than the wild type and have synergistic engulfment defects when certain secondary mutations are introduced (2, 7, 38). Together, these results strongly support a role for the SpoIIQ/SpoIIIAH module in engulfment, demonstrating that the zipper contributes to the efficiency of membrane migration even when the SpoIIDMP module is present and functional. They also suggest that the engulfment machinery displays functional redundancy and that the zipper module provides a backup machinery for membrane migration.The precise role of the SpoIIDMP module during engulfment remains unclear. One model proposes that SpoIID and SpoIIP act as a burnt-bridge Brownian ratchet (Fig. ​(Fig.1B)1B) (1, 7). This model asserts that as SpoIID and SpoIIP degrade peptidoglycan, they eliminate their own enzymatic targets, resulting in the absence of substrate in one direction and therefore, overall movement in the opposite direction. As the enzymes move forward toward new targets, the mother cell membranes are dragged along with them because they are anchored in the membrane. This hypothesis predicts that SpoIID and SpoIIP are processive enzymes and that the SpoIIDMP complex could function as a motor, moving along peptidoglycan as a track and pulling the membranes with it (1, 7). A second model predicts that peptidoglycan degradation could simply remove a steric hindrance to membrane migration (such as links between the forespore membrane and the cell wall) and that some other mechanism provides the force required for membrane migration. Although the SpoIIQ-SpoIIIAH module can contribute to membrane migration, these proteins are not always essential for engulfment in intact cells (7, 38), suggesting that another unidentified system must generate the force required for membrane movement if the DMP module does not act as a burnt-bridge Brownian ratchet. Recent evidence suggests that peptidoglycan biosynthesis, which is localized to the leading edge of the engulfing membrane and necessary for membrane migration in the absence of the SpoIIQ-SpoIIIAH proteins, might be this missing force generating mechanism (26).Both models predict that the activities of SpoIID and SpoIIP are essential for membrane migration. This requirement has been demonstrated for SpoIIP (8) and, while this work was under review, for SpoIID. SpoIID shows no sequence similarity to any characterized enzyme that degrades peptidoglycan and thus constitutes the founding member of a new class of enzymes that remodel peptidoglycan (1, 27). However, SpoIID does show some similarity to B. subtilis LytB (24), a protein that enhances the activity of the amidase LytC (5, 20, 34), while SpoIIP is related to LytC (14). A recent study demonstrated that SpoIIP is both an amidase and endopeptidase and that SpoIID both activates SpoIIP and functions as a lytic transglycosylase, cleaving peptidoglycan between NAG and NAM (27). Together, these two enzymes degrade peptidoglycan into its smallest repeating subunits. However, it remains unclear which of the demonstrated or suggested biochemical functions of SpoIID are required for its various in vivo activities (interaction with SpoIIP, localization, septal thinning, and membrane migration), and it is unclear whether peptidoglycan degradation activity is required throughout engulfment or only for the initial stage of septal thinning.We use site-directed mutagenesis to test the role of 56 conserved amino acids in SpoIID, focusing on hydrophilic amino acids that might be involved in protein-protein interactions and peptidoglycan degradation. We identified one mutation (E88A) that eliminates and three others (R106A, K203A, and D210A) that reduce peptidoglycan degradation activity and show that SpoIID activity is required for the earliest stage of engulfment (septal thinning), as well as throughout membrane migration. Our results confirm and extend those of Morlot et al. (27) and also demonstrate that SpoIID activity is required throughout engulfment. Furthermore, our data indicate that the enzymatically inactive mutant protein (E88A) shows increased septal localization compared to the wild-type protein, suggesting that peptidoglycan degradation contributes to the release of SpoIID from the septum. We propose a modified model for the enzymatic cycle of the SpoIID and SpoIIP complex.     

Proposed mechanisms for red palm oil induced cardioprotection in a model of hyperlipidaemia in the rat

High-cholesterol diets alter myocardial and vascular NO-cGMP signaling and have been implicated in ischaemic/reperfusion injury. We investigated the effects of dietary red palm oil (RPO) containing fatty acids, carotonoids, tocopherols and tocotrienols on myocardial ischaemic tolerance and NO-cGMP pathway function in the rat. Wistar rats were fed a standard rat chow+/-RPO, or a standard rat chow+cholesterol+/-RPO diet. Myocardial mechanical function and NO-cGMP signaling pathway intermediates were determined before, during and after 25 min ischaemia. RPO-supplementation improved aortic output recovery and increased myocardial ischaemic cGMP concentrations. Simulated ischaemia (hypoxia) increased cardiomyocyte nitric oxide levels in the two RPO supplemented groups, but not in control non-supplemented groups. RPO supplementation also increased hypoxic nitric oxide levels in the control diet fed, but not the cholesterol fed rats. These data suggest that dietary RPO may improve myocardial ischaemic tolerance by increasing bioavailability of NO and improving NO-cGMP signaling in the heart.