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91.
Rhett M Rautsaw Tristan D Schramer Rachel Acua Lindsay N Arick Mark DiMeo Kathryn P Mercier Michael Schrum Andrew J Mason Mark J Margres Jason L Strickland Christopher L Parkinson 《Molecular biology and evolution》2021,38(3):745
The migration-selection balance often governs the evolution of lineages, and speciation with gene flow is now considered common across the tree of life. Ecological speciation is a process that can facilitate divergence despite gene flow due to strong selective pressures caused by ecological differences; however, the exact traits under selection are often unknown. The transition from freshwater to saltwater habitats provides strong selection targeting traits with osmoregulatory function. Several lineages of North American watersnakes (Nerodia spp.) are known to occur in saltwater habitat and represent a useful system for studying speciation by providing an opportunity to investigate gene flow and evaluate how species boundaries are maintained or degraded. We use double digest restriction-site associated DNA sequencing to characterize the migration-selection balance and test for evidence of ecological divergence within the Nerodia fasciata-clarkii complex in Florida. We find evidence of high intraspecific gene flow with a pattern of isolation-by-distance underlying subspecific lineages. However, we identify genetic structure indicative of reduced gene flow between inland and coastal lineages suggesting divergence due to isolation-by-environment. This pattern is consistent with observed environmental differences where the amount of admixture decreases with increased salinity. Furthermore, we identify significantly enriched terms related to osmoregulatory function among a set of candidate loci, including several genes that have been previously implicated in adaptation to salinity stress. Collectively, our results demonstrate that ecological differences, likely driven by salinity, cause strong divergent selection which promotes divergence in the N. fasciata-clarkii complex despite significant gene flow. 相似文献
92.
Viere Tobias Amor Ben Berger Nicolas Fanous Ruba Dolfing Arduin Rachel Horta Keller Regula Laurent Alexis Loubet Philippe Strothmann Philip Weyand Steffi Wright Laurie Sonnemann Guido 《The International Journal of Life Cycle Assessment》2021,26(3):511-527
The International Journal of Life Cycle Assessment - Scientific Life Cycle Assessment (LCA) literature provides some examples of LCA teaching in higher education, but not a structured overview of... 相似文献
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Arostegui Martin C. Anderson Christopher M. Benedict Rachel F. Dailey Christopher Fiorenza Evan A. Jahn Abby R. 《Reviews in Fish Biology and Fisheries》2021,31(3):573-598
Reviews in Fish Biology and Fisheries - Recreational fishing is practiced by?~?350 million people globally, and while it historically has been thought to have minimal ecological impact... 相似文献
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Maren de Vries Adil S. Mohamed Rachel A. Prescott Ana M. Valero-Jimenez Ludovic Desvignes Rebecca OConnor Claire Steppan Joseph C. Devlin Ellie Ivanova Alberto Herrera Austin Schinlever Paige Loose Kelly Ruggles Sergei B. Koralov Annaliesa S. Anderson Joseph Binder Meike Dittmann 《Journal of virology》2021,95(10)
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Kathie Y Sun Daniel Oreper Sarah A Schoenrock Rachel McMullan Paola Giusti-Rodríguez Vasyl Zhabotynsky Darla R Miller Lisa M Tarantino Fernando Pardo-Manuel de Villena William Valdar 《Genetics》2021,218(1)
Female mammals are functional mosaics of their parental X-linked gene expression due to X chromosome inactivation (XCI). This process inactivates one copy of the X chromosome in each cell during embryogenesis and that state is maintained clonally through mitosis. In mice, the choice of which parental X chromosome remains active is determined by the X chromosome controlling element (Xce), which has been mapped to a 176-kb candidate interval. A series of functional Xce alleles has been characterized or inferred for classical inbred strains based on biased, or skewed, inactivation of the parental X chromosomes in crosses between strains. To further explore the function structure basis and location of the Xce, we measured allele-specific expression of X-linked genes in a large population of F1 females generated from Collaborative Cross (CC) strains. Using published sequence data and applying a Bayesian “Pólya urn” model of XCI skew, we report two major findings. First, inter-individual variability in XCI suggests mouse epiblasts contain on average 20–30 cells contributing to brain. Second, CC founder strain NOD/ShiLtJ has a novel and unique functional allele, Xceg, that is the weakest in the Xce allelic series. Despite phylogenetic analysis confirming that NOD/ShiLtJ carries a haplotype almost identical to the well-characterized C57BL/6J (Xceb), we observed unexpected patterns of XCI skewing in females carrying the NOD/ShiLtJ haplotype within the Xce. Copy number variation is common at the Xce locus and we conclude that the observed allelic series is a product of independent and recurring duplications shared between weak Xce alleles. 相似文献
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Swojani Shrestha Sonalika Singhal Matthew Kalonick Rachel Guyer Alexis Volkert Seema Somji Scott H. Garrett Donald A. Sens Sandeep K. Singhal 《Journal of cellular and molecular medicine》2021,25(22):10466-10479
Damage to proximal tubules due to exposure to toxicants can lead to conditions such as acute kidney injury (AKI), chronic kidney disease (CKD) and ultimately end-stage renal failure (ESRF). Studies have shown that kidney proximal epithelial cells can regenerate particularly after acute injury. In the previous study, we utilized an immortalized in vitro model of human renal proximal tubule epithelial cells, RPTEC/TERT1, to isolate HRTPT cell line that co-expresses stem cell markers CD133 and CD24, and HREC24T cell line that expresses only CD24. HRTPT cells showed most of the key characteristics of stem/progenitor cells; however, HREC24T cells did not show any of these characteristics. The goal of this study was to further characterize and understand the global gene expression differences, upregulated pathways and gene interaction using scRNA-seq in HRTPT cells. Affymetrix microarray analysis identified common gene sets and pathways specific to HRTPT and HREC24T cells analysed using DAVID, Reactome and Ingenuity software. Gene sets of HRTPT cells, in comparison with publicly available data set for CD133+ infant kidney, urine-derived renal progenitor cells and human kidney-derived epithelial proximal tubule cells showed substantial similarity in organization and interactions of the apical membrane. Single-cell analysis of HRTPT cells identified unique gene clusters associated with CD133 and the 92 common gene sets from three data sets. In conclusion, the gene expression analysis identified a unique gene set for HRTPT cells and narrowed the co-expressed gene set compared with other human renal–derived cell lines expressing CD133, which may provide deeper understanding in their role as progenitor/stem cells that participate in renal repair. 相似文献