全文获取类型
收费全文 | 138篇 |
免费 | 22篇 |
出版年
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 4篇 |
2014年 | 6篇 |
2013年 | 10篇 |
2012年 | 3篇 |
2011年 | 4篇 |
2010年 | 2篇 |
2009年 | 6篇 |
2008年 | 8篇 |
2007年 | 7篇 |
2006年 | 6篇 |
2005年 | 6篇 |
2004年 | 4篇 |
2003年 | 11篇 |
2002年 | 7篇 |
2001年 | 12篇 |
2000年 | 5篇 |
1999年 | 8篇 |
1998年 | 6篇 |
1997年 | 2篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1991年 | 3篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 7篇 |
1985年 | 3篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1981年 | 1篇 |
1980年 | 4篇 |
1977年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有160条查询结果,搜索用时 15 毫秒
151.
152.
Specific fragmentation of tRNA and rRNA at a 7-methylguanine residue in the presence of methylated carrier RNA 总被引:3,自引:0,他引:3
V S Zueva A S Mankin A A Bogdanov L A Baratova 《European journal of biochemistry》1985,146(3):679-687
The reaction of site-specific cleavage of tRNA at a 7-methylguanine residue, including subsequent treatment with sodium borohydride and aniline [Wintermeyer, W. and Zachau, H.G. (1975) FEBS Lett. 58, 306-309], was shown to work only within a certain range of tRNA concentrations (higher than 30 microM). The Escherichia coli 16S rRNA, which contained a unique m7G (position 527), could not be split by this method when taken at any concentration. It was found that the presence of statistically methylated carrier RNA in the reaction mixture at the borohydride stage significantly stimulates site-specific fragmentation of 16S rRNA and 32P-labeled tRNAs. Direct sequencing proved that 16S rRNA and tRNA are cleaved by this procedure successfully at the m7G residue. The E. coli 16S rRNA was preparatively cleaved by the described procedure into two fragments. The 5'-terminal fragment (1-526) and the 3'-terminal fragment (528-1542) were isolated in the pure form and their secondary structure investigated by the circular dichroism method. The results of this study showed that the secondary and tertiary structures of the 5'-terminal one-third of the 16S rRNA are at least as ordered as those of intact 16S rRNA or its 3'-terminal two-thirds. 相似文献
153.
Autoantibodies to neutrophil cytoplasmic antigen-associated vasculitis (AAV) is characterised by inflammation of blood vessels. The introduction of immunosuppressive therapy with glucocorticoids and cyclophosphamide transformed AAV from a fatal condition to a largely treatable condition. Over the past 30 years, considerable progress has been made refining immunosuppressive regimens with a focus on minimising toxicity. There is, however, a high unmet need in the treatment of AAV. A proportion of patients are refractory to current therapies; 50% experience a relapse within 5 years and treatment toxicity contributes to mortality and chronic disability. As knowledge of the pathogenesis of vasculitis grows, it is mirrored by the availability of biological agents, which herald a revolution in the treatment of vasculitis. Lymphocyte-targeted and cytokine-targeted agents have been evaluated for the treatment of AAV and are entering the routine therapeutic arena with the potential to improve patient outcomes. As rare diseases, treatment advances in vasculitis depend on international collaborative research networks both to establish an evidence base for newer agents and to develop recommendations for patient management. 相似文献
154.
155.
Andrey G. Tereshchenkov Malgorzata Dobosz-Bartoszek Ilya A. Osterman James Marks Vasilina A. Sergeeva Pavel Kasatsky Ekaterina S. Komarova Andrey N. Stavrianidi Igor A. Rodin Andrey L. Konevega Petr V. Sergiev Natalia V. Sumbatyan Alexander S. Mankin Alexey A. Bogdanov Yury S. Polikanov 《Journal of molecular biology》2018,430(6):842-852
Antibiotic chloramphenicol (CHL) binds with a moderate affinity at the peptidyl transferase center of the bacterial ribosome and inhibits peptide bond formation. As an approach for modifying and potentially improving properties of this inhibitor, we explored ribosome binding and inhibitory activity of a number of amino acid analogs of CHL. The L-histidyl analog binds to the ribosome with the affinity exceeding that of CHL by 10 fold. Several of the newly synthesized analogs were able to inhibit protein synthesis and exhibited the mode of action that was distinct from the action of CHL. However, the inhibitory properties of the semi-synthetic CHL analogs did not correlate with their affinity and in general, the amino acid analogs of CHL were less active inhibitors of translation in comparison with the original antibiotic. The X-ray crystal structures of the Thermus thermophilus 70S ribosome in complex with three semi-synthetic analogs showed that CHL derivatives bind at the peptidyl transferase center, where the aminoacyl moiety of the tested compounds established idiosyncratic interactions with rRNA. Although still fairly inefficient inhibitors of translation, the synthesized compounds represent promising chemical scaffolds that target the peptidyl transferase center of the ribosome and potentially are suitable for further exploration. 相似文献
156.
H J Mankin 《Federation proceedings》1973,32(4):1478-1480
157.
The molecular organization of the beta-globin complex of the deer mouse, Peromyscus maniculatus 总被引:1,自引:0,他引:1
Padgett RW; Loeb DD; Snyder LR; Edgell MH; Hutchison CA d 《Molecular biology and evolution》1987,4(1):30-45
Recombinant DNA clones have been isolated that contain 80 kb of the
beta-globin complex from the deer mouse, Peromyscus maniculatus.
Comparisons of this complex with that from the laboratory mouse, Mus
domesticus (with an order 5'-Hbby, Hbb-bhO, Hbb-bhl, Hbb-bh2, Hbb-bh3,
Hbb-bl, Hbb-b2 3') highlight organizational trends in the beta-globin
complex since the two species diverged. Unlike other mammals studied thus
far, the deer mouse possesses three adult genes. Partial sequence analysis
indicates that each of the three adult genes is intact and hence may be
functional. Hybridization of one of the two Mus pseudogenes, Hbb-bh3, to
genomic blots from Peromyscus reveals that it has a homologous counterpart
in Peromyscus. Homologous genes to the two gamma-like Mus genes, Hbb-bhO
and Hbb-bhl, are also found in Peromyscus. The strong hybridization between
the Hbb-bhl genes and significant nucleotide similarity between the Hbb-bhO
genes suggest that both pairs are important for the ontogeny of these mice
although no known product has been identified for the Hbb-bhO genes. The
presence of Hbb-bhO and Hbb-bhl in Peromyscus suggests that the duplication
that created this related gene set occurred before the two lineages
diverged. A single gene for Hbb-y has been isolated from Peromyscus. The
adult region in Peromyscus has undergone significant divergence from the
same region in Mus, having three rather than two adult genes, the
acquisition of at least 15 kb of extra DNA relative to Mus, and possibly
the loss of the Hbb-bh2 pseudogene. The nonadult region of the complex, in
contrast, contains the same set of genes apparently distributed over the
same amount of DNA as in the Mus beta- globin complex. This observation
suggests that the embryonic region of the complex is more evolutionarily
stable than the adult region.
相似文献
158.
159.
S. Luke Mankin D. Steven Hill Paula M. Olhoft Effie Toren Allan R. Wenck Lawrence Nea Liqun Xing Jeffrey A. Brown Huihua Fu Lesley Ireland Hongmei Jia Helke Hillebrand Todd Jones Hee-Sook Song 《In vitro cellular & developmental biology. Plant》2007,43(6):521-535
Agrobacterium rhizogenes strain K599 (pRi2659), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of
plant species, including numerous soybean (Glycine max) cultivars. Because Agrobacterium-mediated transformation of soybean remains challenging and labor intensive, K599 appeared a suitable progenitor for new agrobacteria
strains for plant transformation. In this paper, we report the disarming and sequencing of pRi2659 and the usefulness of the
resulting disarmed strain in plant transformation studies of Arabidopsis thaliana, maize (Zea mays), tomato (Lycopersicon esculentum), and soybean (G. max).
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
160.
Unusual nucleotide sequence heterogeneity of small multicopy pHSB plasmid from Halobacterium strain SB3, an archaebacterium 总被引:4,自引:0,他引:4
A small multicopy plasmid, pHSB, from the Halobacterium strain SB3 is present in the cell as a population of molecules differing from each other by one or several point nucleotide substitutions. At least three sequence versions of the plasmid coexist in the cell. The pHSB primary structure heterogeneity may be a reflection of high genetic instability of the host organism. 相似文献