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Alenir Naves de Sales Angélica Cristina de Souza Rondinele de Oliveira Moutta Viridiana Santana Ferreira-Leitão Rosane Freitas Schwan 《Preparative biochemistry & biotechnology》2017,47(5):505-512
Actinobacteria isolates from Brazilian Cerrado soil were evaluated for their ability to produce enzymes of the cellulolytic and xylanolytic complex using lignocellulose residual biomass. Preliminary semiquantitative tests, made in Petri plates containing carboxymethylcellulose and beechwood xylan, indicated 11 potential species producing enzymes, all belonging to the genus Streptomyces. The species were subsequently grown in pure substrates in submerged fermentation and analyzed for the production of enzymes endoglucanase, β-glucosidase, endoxylanase, and β-xylosidase. The best results were obtained for endoxylanase enzyme production with Streptomyces termitum(UFLA CES 93). The strain was grown on lignocellulose biomass (bagasse, straw sugarcane, and cocoa pod husk) that was used in natura or acid pretreated. The medium containing sugarcane bagasse in natura favored the production of the endoxylanase that was subsequently optimized through an experimental model. The highest enzyme production 0.387?U?mL?1, (25.8 times higher), compared to the lowest value obtained in one of the trials, was observed when combining 2.75% sugar cane bagasse and 1.0?g?L?1 of yeast extract to the alkaline medium (pH 9.7). This is the first study using S. termitum as a producer of endoxylanase. 相似文献
63.
H.P. Schwan 《Bioelectromagnetics》1999,20(Z4):3-8
The contributions of Carl Durney to dosimetry have decisively advanced the bioelectromagnetics field and led to significant revisions of relevant health standards. Three items come to mind while studying his work: 1. The work of Carl Durney and his colleagues in dosimetry has advanced the bioelectromagnetics field most significantly whereas more abundant work of a biomedical nature has had less impact. More biophysics work is desirable. 2. The rationale for the specific absorption rate as a basis of health standards needs further elaboration. The need for scaling animal results is stressed. 3. Dosimetry at the cellular level (microdosimetry) is essential if one cares to discuss direct field interactions at the cellular and macromolecular level. Carl Durney's recognition of this need is stated. Carl Durney's wide range of productive interests is indicated by several tables. They summarize his many contributions to electrical engineering, education, bioelectromagnetic dosimetry, hyperthermia, NMR, and field‐induced biophysical phenomena at the molecular and cellular level. His scientific work is summarized, including how his interest changed with time. His scientific accomplishment and productive interaction with students, colleagues, and society sets an example to be admired. Bioelectromagnetics 20:3–8, 1999. © 1999 Wiley‐Liss, Inc. 相似文献
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Lorenzo R. Sewanan Jinkyu Park Michael J. Rynkiewicz Alice W. Racca Nikolaos Papoutsidakis Jonas Schwan Daniel L. Jacoby Jeffrey R. Moore William Lehman Yibing Qyang Stuart G. Campbell 《The Journal of general physiology》2021,153(9)
Hypertrophic cardiomyopathy (HCM) is an inherited disorder caused primarily by mutations to thick and thinfilament proteins. Although thin filament mutations are less prevalent than their oft-studied thick filament counterparts, they are frequently associated with severe patient phenotypes and can offer important insight into fundamental disease mechanisms. We have performed a detailed study of tropomyosin (TPM1) E192K, a variant of uncertain significance associated with HCM. Molecular dynamics revealed that E192K results in a more flexible TPM1 molecule, which could affect its ability to regulate crossbridges. In vitro motility assays of regulated actin filaments containing TPM1 E192K showed an overall loss of Ca2+ sensitivity. To understand these effects, we used multiscale computational models that suggested a subtle phenotype in which E192K leads to an inability to completely inhibit actin–myosin crossbridge activity at low Ca2+. To assess the physiological impact of the mutation, we generated patient-derived engineered heart tissues expressing E192K. These tissues showed disease features similar to those of the patients, including cellular hypertrophy, hypercontractility, and diastolic dysfunction. We hypothesized that excess residual crossbridge activity could be triggering cellular hypertrophy, even if the overall Ca2+ sensitivity was reduced by E192K. To test this hypothesis, the cardiac myosin–specific inhibitor mavacamten was applied to patient-derived engineered heart tissues for 4 d followed by 24 h of washout. Chronic mavacamten treatment abolished contractile differences between control and TPM1 E192K engineered heart tissues and reversed hypertrophy in cardiomyocytes. These results suggest that the TPM1 E192K mutation triggers cardiomyocyte hypertrophy by permitting excess residual crossbridge activity. These studies also provide direct evidence that myosin inhibition by mavacamten can counteract the hypertrophic effects of mutant tropomyosin. 相似文献
67.
Panagiotis Papatheodorou Carsten Schwan Holger Barth Klaus Aktories 《Cellular microbiology》2014,16(4):490-503
TccC3 and TccC5 from Photorhabdus luminescens are ADP‐ribosyltransferases, which modify actin and Rho GTPases, respectively, thereby inducing polymerization and clustering of actin. The bacterial proteins are components of the Photorhabdus toxin complexes, consisting of the binding and translocation component TcdA1, a proposed linker component TcdB2 and the enzymatic component TccC3/5. While the action of the toxins on target proteins is clearly defined, uptake and translocation of the toxins into the cytosol of target cells are not well understood. Here we show by using pharmacological inhibitors that heat shock protein 90 (Hsp90) and peptidyl prolyl cis/trans isomerases (PPIases) including cyclophilins and FK506‐binding proteins (FKBPs) facilitate the uptake of the ADP‐ribosylating toxins into the host cell cytosol. Inhibition of Hsp90 and/or PPIases resulted in decreased intoxication of target cells by Photorhabdus toxin complexes determined by cell rounding and reduction of transepithelial electrical resistance of cell monolayers. ADP‐ribosyltransferase activity of toxins and toxin‐induced pore formation were notimpaired by the inhibitors of Hsp90 and PPIases. The Photorhabdus toxins interacted with Hsp90, FKBP51, Cyp40 and CypA, suggesting a role of these host cell factors in translocation and/or refolding of the ADP‐ribosyltransferases. 相似文献
68.
Whasley Ferreira Duarte Disney Ribeiro Dias Gilberto Vinicius de Melo Pereira Ivani Maria Gervásio Rosane Freitas Schwan 《Journal of industrial microbiology & biotechnology》2009,36(4):557-569
The objectives of this study were to evaluate the potential of gabiroba Campomanesia pubescens (DC) O. Berg in the production of a beverage fermented using selected and wild yeasts from indigenous fermentation, analyze
the volatile compounds profile present during the process of fermentation, and evaluate the sensory quality of the final beverage
produced. Throughout the process of fermentation, when Saccharomyces cerevisiae UFLA CA 1162 was inoculated, there were stable viable populations around 9 log cells ml−1. During indigenous fermentation, yeast population increased from 3.7 log CFU ml−1 to 8.1 log CFU ml−1 after 14 days. The diversity and dynamics of the yeast population during indigenous fermentation observed by PFGE analysis
showed five different karyotyping profiles in the first days of fermentation. After the seventh day, there was a higher frequency
of a similar S. cerevisiae profile. The yeast non-Saccharomyces were identified by sequencing of the ITS region as Candida quercitrusa and Issatchenkia terricola. Inoculated fermentations yielded a higher amount of alcohol than indigenous ones, indicating the efficiency of selected
strains. There was also a greater concentration of higher alcohols, which are usually responsible for the flavor found in
alcoholic beverages. Based on the characteristics of the pulp and acceptance in the sensory analysis, gabiroba fruits showed
good potential for use in the production of fermented beverage. 相似文献
69.
William R Schwan Craig Dunek Michael Gebhardt Kathleen Engelbrecht Tiffany Klett Aaron Monte Joseph Toce Marc Rott Thomas J Volk John J LiPuma Xue-Ting Liu Ronald McKelvey 《Annals of clinical microbiology and antimicrobials》2010,9(1):1-6
Introduction
Antibiotic resistance decreases success of Helicobacter pylori (Hp) eradication. Recently published results show low rate of resistance and better compliance with moxifloxacin based regiments.Aims&methods
Whether 7 days moxifloxacin with lansoprasole and amoxycillin can be compared with 10 days moxifloxacin with lansoprasole and amoxycillin according to moxifloxacin resistance. Patients with non-ulcer dyspepsia who had culture and histology positive Hp infection (n = 150) were randomly assigned into two groups. The first group (n = 75) received moxifloxacin 400 mg/d during 7 days and the other (n = 75) received moxifloxacin 400 mg/d during 10 days. All patients received amoxycillin 1 g twice daily, lansoprasole 30 mg twice daily. All Hp cultures were tested for sensitivity to moxifloxacin.Results
138 patients (92%) completed the study, 68 in the first group and 70 in the second. Eradication rates were 84% (57/68) and 76% (57/75) in the 7 days moxifloxacin group and 90% and 84% in the second group (63/70, 63/75) according to the PP and ITT analysis; p = n.s. Among 129 patients (86% of study group), 6% of strains were primary resistant to moxifloxacin. Eradication of moxifloxacin sensitive/resistant strains was 98%/66%, p < 0.05Conclusion
According to our results we recommend 7 days moxiflixacin based triple therapy. 相似文献70.
Dai Q Restrepo BI Porcella SF Raffel SJ Schwan TG Barbour AG 《Molecular microbiology》2006,60(6):1329-1343
The relapsing fever agent Borrelia hermsii undergoes multiphasic antigenic variation through gene conversion of a unique expression site on a linear plasmid by an archived variable antigen gene. To further characterize this mechanism we assessed the repertoire and organization of archived variable antigen genes by sequencing approximately 85% of plasmids bearing these genes. Most archived genes shared with the expressed gene a 相似文献