蛋白质芯片技术检测脑损伤大鼠血清差异蛋白

目的:研究大鼠脑损伤后血清中蛋白质表达谱的变化及其特点.方法:采用弱阳离子交换芯片(WCX2)结合表面增强激光解析电离飞行时间质谱技术分析大鼠闭合性脑损伤后4 h、8 h、12 h、24 h、48 h血清中蛋白质表达谱的改变.结果:与对照组相比,脑损伤后血清中有2个蛋白质的表达谱发生改变.其中差异蛋白5648Da,在4 h、8 h和12 h组表达降低(P＜0.01),24 h和48 h组表达恢复;差异蛋白9681Da在对照组、24 h和48 h组几乎不表达,而4 h、8 h和12 h组表达增加(P＜0.05).结论:脑损伤可引起血清中蛋白质表达谱发生变化.     

A putative NES mediates cytoplasmic localization of Apoptin in normal cells

Chicken anemia virus (CAV) is a small non-envelopedvirus containing a single-stranded circular DNA genome.The virus causes a disease in the newborn chickens, whichis characterized by generalized lymphoid atrophy, increasedmortality and severe anemia. CAV …     

骨与关节系统发生过程中相关调控机制的研究进展

脊椎动物胚胎期骨与关节系统的发生是一种复杂生命现象,起始于中胚层间充质细胞的定向聚集,形成肢芽,然后在一系列作用因子的调控下,肢芽内细胞进一步分化,形成具有骨骼雏形的软骨原基,后者经软骨内骨化发育成骨。四肢骨大多是以这种方式发生的,四肢的滑膜关节系统也随骨骼的发生而形成。详细阐述了近年来对肢体骨与关节系统发生各步骤相关调控机制方面的研究进展。Abstract: The embryonic development of bone and joint involves in complicated events for vertebrate limb. It originates from determined condensation of mesenchymal cells from lateral mesoderm. These cells and the overlying ectodermal jacket form limb buds at presumptive limb levels. Then, under the control of systemic factors, mesenchymal cells aggregate and differentiate to form catilage blastemal elements that prefigure skeletal limb components. The latter develops into skeleton through endochondral ossification. The majority of the bones of the limb form by the endochondral mechanism. The formation of synovial joint system and bone development occur simultaneously. This article reviewed the progress on the related control mechanism in the development of bone and joint recently.     

WNT家族在脊椎动物骨骼发育中的作用机制

脊椎动物骨骼系统起源于中胚层间充质细胞,起初,这些细胞定向分化形成软骨原基,后者经软骨内骨化发育为成熟的骨骼系统。近年来,很多研究表明,WNT家族与其相关作用成分在骨发育过程中发挥了重要作用,通过在细胞分化不同阶段的正向或负向调控机制,保证了软骨细胞在特定的位置以合适的速率有序分化。在WNT家族及其作用途径的相关信号分子中,无论何种亚型或分子的异常表达都可能破坏WNT系统维系的正负平衡机制,导致骨骼系统畸形。了解WNT系统的作用机制有助于深入探究骨骼系统发生的相关调控机理。     

红蓝光质对芹菜碳氮代谢及其关键酶活性的影响

为了探明红蓝光对芹菜生长的调控机理,以‘津南实芹1号’为试材,白光(W)处理为对照(CK),研究红蓝LED光质对芹菜碳氮代谢及其相关酶活性的影响。结果表明：白+红(WR)和白+红+蓝(WRB)处理芹菜的光合速率(Pn)及RuBP羧化酶(RuBPCase)活性显著高于CK,白+蓝(WB)处理的Pn低于CK,但RuBPCase活性高于CK。各处理相比,碳代谢产物多以WR处理的最高, WRB处理的其次, WB处理的最低。WR处理的蔗糖磷酸合成酶(SPS)活性显著高于CK,蔗糖合成酶(SS)活性明显低于CK;WB处理的SS活性较高。WR处理的总氮含量及谷氨酰胺酶(GS)活性显著高于CK,而蛋白质和氨基酸含量明显低于CK; WB处理的蛋白质含量较高,氨基酸含量较低,硝酸还原酶(NR)、GS和谷氨酸合成酶(GOGAT)活性明显高于CK;WRB处理的总氮和蛋白质含量及NR和GS活性均高于CK。可见,增加红光比例可促进芹菜碳的同化、转化及氮的吸收,加速物质积累,但对蛋白质的合成有一定的抑制作用;增加蓝光比例可使芹菜氮代谢增强,但碳的积累代谢下降,因此叶柄伸长受抑,单株产量降低。     

Low oleic acid-derived repression of jasmonic acid-inducible defense responses requires the WRKY50 and WRKY51 proteins

Signaling induced upon a reduction in oleic acid (18:1) levels simultaneously up-regulates salicylic acid (SA)-mediated responses and inhibits jasmonic acid (JA)-inducible defenses, resulting in enhanced resistance to biotrophs but increased susceptibility to necrotrophs. SA and the signaling component Enhanced Disease Susceptibility1 function redundantly in this low-18:1-derived pathway to induce SA signaling but do not function in the repression of JA responses. We show that repression of JA-mediated signaling under low-18:1 conditions is mediated via the WRKY50 and WRKY51 proteins. Knockout mutations in WRKY50 and WRKY51 lowered SA levels but did not restore pathogenesis-related gene expression or pathogen resistance to basal levels in the low-18:1-containing Arabidopsis (Arabidopsis thaliana) mutant, suppressor of SA insensitivity2 (ssi2). In contrast, both JA-inducible PDF1.2 (defensin) expression and basal resistance to Botrytis cinerea were restored. Simultaneous mutations in both WRKY genes (ssi2 wrky50 wrky51) did not further enhance the JA or Botrytis-related responses. The ssi2 wrky50 and ssi2 wrky51 plants contained high levels of reactive oxygen species and exhibited enhanced cell death, the same as ssi2 plants. This suggested that high reactive oxygen species levels or increased cell death were not responsible for the enhanced susceptibility of ssi2 plants to B. cinerea. Exogenous SA inhibited JA-inducible PDF1.2 expression in the wild type but not in wrky50 or wrky51 mutant plants. These results show that the WRKY50 and WRKY51 proteins mediate both SA- and low-18:1-dependent repression of JA signaling.     

光学成像技术在体研究肿瘤的光动力效应

光动力疗法 (PDT) 已发展成为一种较成熟的肿瘤治疗方法, PDT 诱导的血管损伤是杀死肿瘤的重要机制之一 . 为了在活体肿瘤模型上实时监测 PDT 导致的血管损伤效应,使用稳定高表达绿色荧光蛋白 (GFP) 的人涎腺腺样囊性癌细胞株 (ACC-M-GFP) ,建立了基于鸡胚尿囊膜 (CAM) 的肿瘤模型 . 应用荧光成像技术对肿瘤的生长位置、大小,以及治疗区域进行方便精确的定位;利用激光散斑成像技术,实时监测 CAM 上肿瘤周围血管的血液动力学参数 . 发现不同光动力剂量所导致的血管损伤有显著不同 . 结果表明,荧光标记的鸡胚尿囊膜肿瘤模型为研究 PDT 导致的血管损伤效应提供了良好的实验模型,激光散斑成像技术适用于实时监测 PDT 过程中血管结构、血流速度的变化,由此得出血液灌注率可用以评估 PDT 对肿瘤周围血管的损伤效应 .     

B细胞表位的计算机预测

B细胞表位预测对于多种免疫学研究是必不可少的重要工具.本文概括了计算机预测B细胞表位的现状,汇总了多种表位预测工具及其原理和应用,介绍了一些用于建立与评价预测工具的数据库和数据集,对各类预测工具和数据库的特点和网址进行整理列表.另外,还分析了B细胞表位预测领域存在的问题,并对其未来发展提出了建议.