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121.
Retinoic acid-mediated activation of HNF-3 alpha during EC stem cell differentiation. 总被引:2,自引:0,他引:2 下载免费PDF全文
A Jacob S Budhiraja X Qian D Clevidence R H Costa R R Reichel 《Nucleic acids research》1994,22(11):2126-2133
122.
Purification and characterization of two phosphoglucomutases from Lactococcus lactis subsp. lactis and their regulation in maltose- and glucose-utilizing cells. 下载免费PDF全文
Two distinct forms of phosphoglucomutase were found in Lactococcus lactis subsp. lactis, strains 19435 and 65.1, growing on maltose: beta-phosphoglucomutase (beta-PGM), which catalyzes the reversible conversion of beta-glucose 1-phosphate to glucose 6-phosphate in the maltose catabolism, and alpha-phosphoglucomutase (alpha-PGM). beta-PGM was purified to more than 90% homogeneity in crude cell extract from maltose-grown lactococci, and polyclonal antisera to the enzyme were prepared. The molecular mass of beta-PGM was estimated by gel filtration to be 28 kDa; its isoelectric point was 4.8. The corresponding values for alpha-PGM were 65 kDa and 4.4, respectively. The expression of both PGM enzymes was investigated under different growth conditions. The specific activity and amount of beta-PGM per milliliter of cell extract increased with time in lactococci grown on maltose, but the enzyme was absent in lactococci grown on glucose, indicating enzyme synthesis to be induced by maltose in the growth medium. When glucose was added to maltose-grown lactococci, both the specific activity and amount of beta-PGM per milliliter of cell extract decreased rapidly. This suggests that synthesis of beta-PGM is repressed by glucose in the medium. Although the specific activity of alpha-PGM did not change during growth on maltose or glucose, lactococcal strain 19435 showed a much higher specific activity of both alpha- and beta-PGM than strain 65.1 when grown on maltose. 相似文献
123.
Saturated Molecular Map of the Rice Genome Based on an Interspecific Backcross Population 总被引:50,自引:0,他引:50 下载免费PDF全文
M. A. Causse T. M. Fulton Y. G. Cho S. N. Ahn J. Chunwongse K. Wu J. Xiao Z. Yu P. C. Ronald S. E. Harrington G. Second S. R. McCouch S. D. Tanksley 《Genetics》1994,138(4):1251-1274
A molecular map has been constructed for the rice genome comprised of 726 markers (mainly restriction fragment length polymorphisms; RFLPs). The mapping population was derived from a backcross between cultivated rice, Oryza sativa, and its wild African relative, Oryza longistaminata. The very high level of polymorphism between these species, combined with the use of polymerase chain reaction-amplified cDNA libraries, contributed to mapping efficiency. A subset of the probes used in this study was previously used to construct an RFLP map derived from an inter subspecific cross, providing a basis for comparison of the two maps and of the relative mapping efficiencies in the two crosses. In addition to the previously described PstI genomic rice library, three cDNA libraries from rice (Oryza), oat (Avena) and barley (Hordeum) were used in this mapping project. Levels of polymorphism detected by each and the frequency of identifying heterologous sequences for use in rice mapping are discussed. Though strong reproductive barriers isolate O. sativa from O. longistaminata, the percentage of markers showing distorted segregation in this backcross population was not significantly different than that observed in an intraspecific F(2) population previously used for mapping. The map contains 1491 cM with an average interval size of 4.0 cM on the framework map, and 2.0 cM overall. A total of 238 markers from the previously described PstI genomic rice library, 250 markers from a cDNA library of rice (Oryza), 112 cDNA markers from oat (Avena), and 20 cDNA markers from a barley (Hordeum) library, two genomic clones from maize (Zea), 11 microsatellite markers, three telomere markers, eleven isozymes, 26 cloned genes, six RAPD, and 47 mutant phenotypes were used in this mapping project. Applications of a molecular map for plant improvement are discussed. 相似文献
124.
John A. Lowe III Weimin Qian Pamela J. Scott Stafford McLean Dianne K. Bryce Rosemary T. Crawford Jon Bordner 《Bioorganic & medicinal chemistry letters》1994,4(24):2877-2882
A series of 5,7-diphenyl-3-ureidohexahydroazepin-2-one cholecystokinin-B (CCK-B) receptor antagonists was synthesized using Beckmann ring expansion of a suitable 2,4-diphenylcyclohexanone as a key step. SAR studies revealed the importance of the 5-aryl group for high and selective CCK-B receptor affinity, as illustrated in compound (−)-10i (CCK-B IC50 = 6.8 nM). 相似文献
125.
Identification of amino acid residues involved in the binding of Huperzine A to cholinesterases. 总被引:2,自引:0,他引:2 下载免费PDF全文
A. Saxena N. Qian I. M. Kovach A. P. Kozikowski Y. P. Pang D. C. Vellom Z. Radi D. Quinn P. Taylor B. P. Doctor 《Protein science : a publication of the Protein Society》1994,3(10):1770-1778
Huperzine A, a potential agent for therapy in Alzheimer's disease and for prophylaxis of organophosphate toxicity, has recently been characterized as a reversible inhibitor of cholinesterases. To examine the specificity of this novel compound in more detail, we have examined the interaction of the 2 stereoisomers of Huperzine A with cholinesterases and site-specific mutants that detail the involvement of specific amino acid residues. Inhibition of fetal bovine serum acetylcholinesterase by (-)-Huperzine A was 35-fold more potent than (+)-Huperzine A, with KI values of 6.2 nM and 210 nM, respectively. In addition, (-)-Huperzine A was 88-fold more potent in inhibiting Torpedo acetylcholinesterase than (+)-Huperzine A, with KI values of 0.25 microM and 22 microM, respectively. Far larger KI values that did not differ between the 2 stereoisomers were observed with horse and human serum butyrylcholinesterases. Mammalian acetylcholinesterase, Torpedo acetylcholinesterase, and mammalian butyrylcholinesterase can be distinguished by the amino acid Tyr, Phe, or Ala in the 330 position, respectively. Studies with mouse acetylcholinesterase mutants, Tyr 337 (330) Phe and Tyr 337 (330) Ala yielded a difference in reactivity that closely mimicked the native enzymes. In contrast, mutation of the conserved Glu 199 residue to Gln in Torpedo acetylcholinesterase produced only a 3-fold increase in KI value for the binding of Huperzine A.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
126.
河南叶县下寒武统辛集组单板类和腹足类化石的研究 总被引:8,自引:3,他引:5
本文描述和讨论了河南叶县杨寺庄下寒武统辛集组单板类13种,腹足类2种,未定类1种。其中包括2新属6新种:Repenoconus xinjiensis gen.nov.,Scenella pycna sp.nov.,Securiconus vulgaris sp.nov.,Obtusocnus grossicostus sp.nov.,Igorellina proboscis sp.nov.,Gal 相似文献
127.
云南西双版纳桑寄生植物传播与鸟的关系研究 总被引:18,自引:2,他引:16
在西双版纳地区桑寄生植物的种子是鸟类传播。传播的鸟类主要是啄花鸟科的纯色啄花鸟,红胸啄花鸟,朱背啄花鸟和黄肛啄花鸟等。桑寄生植物种子的传播方式,一是鸟类蚕食除去外果皮的种子或外果皮果肉相互连着的种子,经消化道消化吸收大部分果肉或外果皮后,将种子排出体外,其次是鸟在觅食中遗漏种子进行传播。 相似文献
128.
云南阿昌族的红细胞血型分布 总被引:2,自引:2,他引:0
调查了102名云南阿昌族的ABO,NNSs,Rh和P系统的红细胞血型,结果表明,阿昌族的基因频率p(0.3874)是迄今国内调查过人群中的最高值,E(0.2459)和CDe(0.6936)基因或染色体频率较高,而S(0.0686)和P1(0.1089)频率较低;Ms(0.5950)连锁率高于Ns(0.3353);未发现SS和Rh(-D)阴性表现型。 相似文献
129.
西洋参胚性细胞和胚状体细胞中的核内含体与细胞质内含体 总被引:1,自引:0,他引:1
1921年,Molish首先在光镜下发现植物叶片细胞核中有一种晶体状的内含物(见Weintraub等[1])。后来人们陆续在某些动、植物的细胞核中观察到了这种结构[2—4],并称之为核内含体(intranuclearinclusions)。Bigazzi[3]曾在45种桔梗科植物的细胞中看到了核内含体。但在离体培养的植物细胞中发现内含体的报道很少,至今仅在榛子组织培养分生细胞中看到了类似的结构[5]。我们在对西洋参体细胞胚胎发生进行超微结构研究的过程中发现,在胚性愈伤组织和胚状体细胞的核和细胞质… 相似文献
130.
水平回转对水稻幼苗叶细胞的影响 总被引:9,自引:0,他引:9
对在模拟微重力装置上回转14 天的水稻幼苗叶细胞进行了亚显微形态、电子探针和细胞酶化学研究。发现叶细胞质膜上Ca2+ -ATP酶活性消失,膜内钙总量上升、膜外钙总量下降,细胞骨架变得疏松,细胞壁变薄并凹凸不平。叶绿体的基粒和线粒体的内嵴亦有部分变化。其变化机制,首先是细胞质膜上Ca2+ -ATP酶活性消失,膜上钙泵停止工作,跨膜钙浓度差减小,膜内钙浓度上升,微管、微丝聚合受阻,细胞骨架疏松,分泌泡移动失去导向,从而导致细胞壁变薄等状态 相似文献