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91.
Belowground herbivores can exert important controls on the composition of natural plant communities. Until now, relatively
few studies have investigated which factors may control the abundance of belowground herbivores. In Dutch coastal foredunes,
the root-feeding nematode Tylenchorhynchus ventralis is capable of reducing the performance of the dominant grass Ammophila arenaria (Marram grass). However, field surveys show that populations of this nematode usually are controlled to nondamaging densities,
but the control mechanism is unknown. In the present study, we first established that T. ventralis populations are top-down controlled by soil biota. Then, selective removal of soil fauna suggested that soil microorganisms
play an important role in controlling T. ventralis. This result was confirmed by an experiment where selective inoculation of microarthropods, nematodes and microbes together
with T. ventralis into sterilized dune soil resulted in nematode control when microbes were present. Adding nematodes had some effect, whereas
microarthropods did not have a significant effect on T. ventralis. Our results have important implications for the appreciation of herbivore controls in natural soils. Soil food web models
assume that herbivorous nematodes are controlled by predaceous invertebrates, whereas many biological control studies focus
on managing nematode abundance by soil microorganisms. We propose that soil microorganisms play a more important role than
do carnivorous soil invertebrates in the top-down control of herbivorous ectoparasitic nematodes in natural ecosystems. This
is opposite to many studies on factors controlling root-feeding insects, which are supposed to be controlled by carnivorous
invertebrates, parasitoids, or entomopathogenic nematodes. Our conclusion is that the ectoparasitic nematode T. ventralis is potentially able to limit productivity of the dune grass A. arenaria but that soil organisms, mostly microorganisms, usually prevent the development of growth-reducing population densities. 相似文献
92.
Farmery A. K. Alexander K. Anderson K. Blanchard J. L. Carter C. G. Evans K. Fischer M. Fleming A. Frusher S. Fulton E. A. Haas B. MacLeod C. K. Murray L. Nash K. L. Pecl G. T. Rousseau Y. Trebilco R. van Putten I. E. Mauli S. Dutra L. Greeno D. Kaltavara J. Watson R. Nowak B. 《Reviews in Fish Biology and Fisheries》2022,32(1):101-121
Reviews in Fish Biology and Fisheries - Food from the sea can make a larger contribution to healthy and sustainable diets, and to addressing hunger and malnutrition, through improvements in... 相似文献
93.
Melbourne-Thomas Jess Audzijonyte Asta Brasier Madeleine J. Cresswell Katherine A. Fogarty Hannah E. Haward Marcus Hobday Alistair J. Hunt Heather L. Ling Scott D. McCormack Phillipa C. Mustonen Tero Mustonen Kaisu Nye Janet A. Oellermann Michael Trebilco Rowan van Putten Ingrid Villanueva Cecilia Watson Reg A. Pecl Gretta T. 《Reviews in Fish Biology and Fisheries》2022,32(1):231-251
Reviews in Fish Biology and Fisheries - One of the most pronounced effects of climate change on the world’s oceans is the (generally) poleward movement of species and fishery stocks in... 相似文献
94.
Novaglio Camilla Bax Narissa Boschetti Fabio Emad Gholam Reza Frusher Stewart Fullbrook Liam Hemer Mark Jennings Sarah van Putten Ingrid Robinson Lucy M. Spain Erica Vince Joanna Voyer Michelle Wood Graham Fulton Elizabeth A. 《Reviews in Fish Biology and Fisheries》2022,32(1):209-230
Reviews in Fish Biology and Fisheries - The ocean economy is experiencing rapid growth that will provide benefits but will also pose environmental and social risks. With limited space and degraded... 相似文献
95.
96.
Bas-Jan Zandt Sid Visser Michel J. A. M. van Putten Bennie ten Haken 《Journal of computational neuroscience》2014,37(3):549-568
Neural mass models are successful in modeling brain rhythms as observed in macroscopic measurements such as the electroencephalogram (EEG). While the synaptic current is explicitly modeled in current models, the single cell electrophysiology is not taken into account. To allow for investigations of the effects of channel pathologies, channel blockers and ion concentrations on macroscopic activity, we formulate neural mass equations explicitly incorporating the single cell dynamics by using a bottom-up approach. The mean and variance of the firing rate and synaptic input distributions are modeled. The firing rate curve (F(I)-curve) is used as link between the single cell and macroscopic dynamics. We show that this model accurately reproduces the behavior of two populations of synaptically connected Hodgkin-Huxley neurons, also in non-steady state. 相似文献
97.
Stewart D. Frusher Alistair J. Hobday Sarah M. Jennings Colin Creighton Dallas D’Silva Marcus Haward Neil J. Holbrook Melissa Nursey-Bray Gretta T. Pecl E. Ingrid van Putten 《Reviews in Fish Biology and Fisheries》2014,24(2):593-611
Climate change is not being felt equally around the world. Regions where warming is most rapid will be among those to experience impacts first, will need to develop early responses to these impacts and can provide a guide for management elsewhere. We describe the research history in one such global marine hotspot—south-east Australia—where a number of contentions about the value of hotspots as natural laboratories have been supported, including (1) early reporting of changes (2) early documentation of impacts, and (3) earlier development and promotion of adaptation options. We illustrate a transition from single discipline impacts-focused research to an inter-disciplinary systems view of adaptation research. This transition occurred against a background of change in the political position around climate change and was facilitated by four preconditioning factors. These were: (1) early observations of rapid oceanic change that coincided with (2) biological change which together provided a focus for action, (3) the strong marine orientation and history of management in the region, and (4) the presence of well developed networks. Three case studies collectively show the critical role of inter-disciplinary engagement and stakeholder participation in supporting industry and government adaptation planning. 相似文献
98.
Anna D. Grabowska Ewa Wywia? Stanislaw Dunin-Horkawicz Anna M. ?asica Marc M. S. M. W?sten Anna Nagy-Staroń Renata Godlewska Katarzyna Bocian-Ostrzycka Katarzyna Pieńkowska Pawe? ?aniewski Janusz M. Bujnicki Jos P. M. van Putten E. Katarzyna Jagusztyn-Krynicka 《PloS one》2014,9(9)
Background
Bacterial Dsb enzymes are involved in the oxidative folding of many proteins, through the formation of disulfide bonds between their cysteine residues. The Dsb protein network has been well characterized in cells of the model microorganism Escherichia coli. To gain insight into the functioning of the Dsb system in epsilon-Proteobacteria, where it plays an important role in the colonization process, we studied two homologs of the main Escherichia coli Dsb oxidase (EcDsbA) that are present in the cells of the enteric pathogen Campylobacter jejuni, the most frequently reported bacterial cause of human enteritis in the world.Methods and Results
Phylogenetic analysis suggests the horizontal transfer of the epsilon-Proteobacterial DsbAs from a common ancestor to gamma-Proteobacteria, which then gave rise to the DsbL lineage. Phenotype and enzymatic assays suggest that the two C. jejuni DsbAs play different roles in bacterial cells and have divergent substrate spectra. CjDsbA1 is essential for the motility and autoagglutination phenotypes, while CjDsbA2 has no impact on those processes. CjDsbA1 plays a critical role in the oxidative folding that ensures the activity of alkaline phosphatase CjPhoX, whereas CjDsbA2 is crucial for the activity of arylsulfotransferase CjAstA, encoded within the dsbA2-dsbB-astA operon.Conclusions
Our results show that CjDsbA1 is the primary thiol-oxidoreductase affecting life processes associated with bacterial spread and host colonization, as well as ensuring the oxidative folding of particular protein substrates. In contrast, CjDsbA2 activity does not affect the same processes and so far its oxidative folding activity has been demonstrated for one substrate, arylsulfotransferase CjAstA. The results suggest the cooperation between CjDsbA2 and CjDsbB. In the case of the CjDsbA1, this cooperation is not exclusive and there is probably another protein to be identified in C. jejuni cells that acts to re-oxidize CjDsbA1. Altogether the data presented here constitute the considerable insight to the Epsilonproteobacterial Dsb systems, which have been poorly understood so far. 相似文献99.
Lieke B. van Alphen Sara A. Burt Andreas K. J. Veenendaal Nancy M. C. Bleumink-Pluym Jos P. M. van Putten 《PloS one》2012,7(9)
Background
Natural compounds with anti-microbial properties are attractive reagents to reduce the use of conventional antibiotics. Carvacrol, the main constituent of oregano oil, inhibits the growth of a variety of bacterial foodborne pathogens. As concentrations of carvacrol may vary in vivo or when used in animal feed, we here investigated the effect of subinhibitory concentrations of the compound on major virulence traits of the principal bacterial foodborne pathogen Campylobacter jejuni.Methods/Principal Findings
Motility assays revealed that subinhibitory concentrations of carvacrol inhibited the motility of C. jejuni without affecting bacterial growth. Immunoblotting and electron microscopy showed that carvacrol-treated C. jejuni still expressed flagella. The loss of motility was not caused by reduced intracellular ATP levels. In vitro infection assays demonstrated that subinhibitory concentrations of carvacrol also abolished C. jejuni invasion of human epithelial cells. Bacterial uptake of invasive Escherichia coli was not blocked by carvacrol. Exposure of C. jejuni to carvacrol prior to infection also inhibited cellular infection, indicating that the inhibition of invasion was likely caused by an effect on the bacteria rather than inhibition of epithelial cell function.Conclusions/Significance
Bacterial motility and invasion of eukaryotic cells are considered key steps in C. jejuni infection. Our results indicate that subinhibitory concentrations of carvacrol effectively block these virulence traits by interfering with flagella function without disturbing intracellular ATP levels. These results broaden the spectrum of anti-microbial activity of carvacrol and support the potential of the compound for use in novel infection prevention strategies. 相似文献100.
Herzig MC Bidinosti M Schweizer T Hafner T Stemmelen C Weiss A Danner S Vidotto N Stauffer D Barske C Mayer F Schmid P Rovelli G van der Putten PH Shimshek DR 《PloS one》2012,7(5):e36581
The G2019S mutation in the multidomain protein leucine-rich repeat kinase 2 (LRRK2) is one of the most frequently identified genetic causes of Parkinson's disease (PD). Clinically, LRRK2(G2019S) carriers with PD and idiopathic PD patients have a very similar disease with brainstem and cortical Lewy pathology (α-synucleinopathy) as histopathological hallmarks. Some patients have Tau pathology. Enhanced kinase function of the LRRK2(G2019S) mutant protein is a prime suspect mechanism for carriers to develop PD but observations in LRRK2 knock-out, G2019S knock-in and kinase-dead mutant mice suggest that LRRK2 steady-state abundance of the protein also plays a determining role. One critical question concerning the molecular pathogenesis in LRRK2(G2019S) PD patients is whether α-synuclein (aSN) has a contributory role. To this end we generated mice with high expression of either wildtype or G2019S mutant LRRK2 in brainstem and cortical neurons. High levels of these LRRK2 variants left endogenous aSN and Tau levels unaltered and did not exacerbate or otherwise modify α-synucleinopathy in mice that co-expressed high levels of LRRK2 and aSN in brain neurons. On the contrary, in some lines high LRRK2 levels improved motor skills in the presence and absence of aSN-transgene-induced disease. Therefore, in many neurons high LRRK2 levels are well tolerated and not sufficient to drive or exacerbate neuronal α-synucleinopathy. 相似文献