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81.
Zameer Ahmed Manickam Gurusaran Prasanth Narayana Kala Sekar Dinesh Kumar Jayapal Mohanapriya Marthandan Kirti Vaishnavi Kanagaraj Sekar 《Bioinformation》2014,10(1):48-51
The primary structure of a protein molecule comprises a linear chain of amino acid residues. Certain parts of this linear chain are
unique in nature and function. They can be classified under different categories and their roles studied in detail. Two such unique
categories are the palindromic sequences and the Single Amino Acid Repeats (SAARs), which plays a major role in the structure,
function and evolution of the protein molecule. In spite of their presence in various protein sequences, palindromes have not yet
been investigated in detail. Thus, to enable a comprehensive understanding of these sequences, a computing engine, PPS, has been
developed. The users can search the occurrences of palindromes and SAARs in all the protein sequences available in various
databases and can view the three-dimensional structures (in case it is available in the known three-dimensional protein structures
deposited to the Protein Data Bank) using the graphics plug-in Jmol. The proposed server is the first of its kind and can be freely
accessed through the World Wide Web.
Availability
URL http://pranag.physics.iisc.ernet.in/pps/ 相似文献82.
Sravan Kumar Jami Greg B. Clark Belay T. Ayele Paula Ashe Pulugurtha Bharadwaja Kirti 《PloS one》2012,7(11)
Most annexins are calcium-dependent, phospholipid-binding proteins with suggested functions in response to environmental stresses and signaling during plant growth and development. They have previously been identified and characterized in Arabidopsis and rice, and constitute a multigene family in plants. In this study, we performed a comparative analysis of annexin gene families in the sequenced genomes of Viridiplantae ranging from unicellular green algae to multicellular plants, and identified 149 genes. Phylogenetic studies of these deduced annexins classified them into nine different arbitrary groups. The occurrence and distribution of bona fide type II calcium binding sites within the four annexin domains were found to be different in each of these groups. Analysis of chromosomal distribution of annexin genes in rice, Arabidopsis and poplar revealed their localization on various chromosomes with some members also found on duplicated chromosomal segments leading to gene family expansion. Analysis of gene structure suggests sequential or differential loss of introns during the evolution of land plant annexin genes. Intron positions and phases are well conserved in annexin genes from representative genomes ranging from Physcomitrella to higher plants. The occurrence of alternative motifs such as K/R/HGD was found to be overlapping or at the mutated regions of the type II calcium binding sites indicating potential functional divergence in certain plant annexins. This study provides a basis for further functional analysis and characterization of annexin multigene families in the plant lineage. 相似文献
83.
Baweja RB Zaman MS Mattoo AR Sharma K Tripathi V Aggarwal A Dubey GP Kurupati RK Ganguli M Chaudhury NK Sen S Das TK Gade WN Singh Y 《Archives of microbiology》2008,189(1):71-79
Bacillus anthracis makes highly stable, heat-resistant spores which remain viable for decades. Effect of various stress conditions on sporulation
in B. anthracis was studied in nutrient-deprived and sporulation medium adjusted to various pH and temperatures. The results revealed that
sporulation efficiency was dependent on conditions prevailing during sporulation. Sporulation occurred earlier in culture
sporulating at alkaline pH or in PBS than control. Spores formed in PBS were highly sensitive towards spore denaturants whereas,
those formed at 45°C were highly resistant. The decimal reduction time (D-10 time) of the spores formed at 45°C by wet heat,
2 M HCl, 2 M NaOH and 2 M H2O2 was higher than the respective D-10 time for the spores formed in PBS. The dipicolinic acid (DPA) content and germination
efficiency was highest in spores formed at 45°C. Since DPA is related to spore sensitivity towards heat and chemicals, the
increased DPA content of spores prepared at 45°C may be responsible for increased resistance to wet heat and other denaturants.
The size of spores formed at 45°C was smallest amongst all. The study reveals that temperature, pH and nutrient availability
during sporulation affect properties of B. anthracis spores. 相似文献
84.
Shekhawat Kirti Bauer Florian F. Setati Mathabatha E. 《Applied microbiology and biotechnology》2017,101(6):2479-2491
Applied Microbiology and Biotechnology - The sequential or co-inoculation of grape must with non-Saccharomyces yeast species and Saccharomyces cerevisiae wine yeast strains has recently become a... 相似文献
85.
Protoplasts derived from hypocotyls of seedlings grown on half-strength MS medium containing 1% sucrose were cultured at a density of 5×104 ml-1 in Kao's medium supplemented with 1.0 mgl-12,4-D, 0.1 mgl-1 NAA and 0.5 mgl-1 zeatin riboside. After three days of culture in darkness at 25±1°C, cultures were transferred to light (70 Em-2s-1) in a 16/8 h ligø ht/dark cycle. Cultures were diluted on the 7th, 10th and 13th day with Kao's medium containing 3.4% sucrose, 0.1 mgl-1 2,4-dichlorophenoxyacetic acid and 1.0 mgl-1 benzyladenine. On the fifteenth day, microcalli were plated on K3 medium gelled with 0.5% agarose (Type 1, low EEO, Sigma). After a further period of two weeks, transfers were made to specific media to achieve either organogenesis or somatic embryogenesis. Time taken from plating protoplasts to obtaining plantlets is 8–10 weeks. Using this procedure, several hundred regenerated plants have been hardened in a growth chamber and transferred to soil. 相似文献
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89.
Praveen Kumar Guttula Kirti Agrawal Pradeep Natarajan 《Journal of biomolecular structure & dynamics》2020,38(16):4947-4955
AbstractCommunicated by Ramaswamy H. Sarma 相似文献
90.
The free radical scavenging potential of the plant S. indicus was studied by using different antioxidant models of screening. The ethanolic extract at 1000 microg/ml showed maximum scavenging of the radical cation, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) observed upto 41.99% followed by the scavenging of the stable radical 1,1-diphenyl, 2-picryl hydrazyl (DPPH) (33.27%), superoxide dismutase (25.14%) and nitric oxide radical (22.36%) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (14.2%). Total antioxidant capacity of the extract was found to be 160.85 nmol/g ascorbic acid. The results justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value. 相似文献