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101.
102.
Anne Popescu Anna-Britta Hörnfeldt Salo Gronowitz 《Nucleosides, nucleotides & nucleic acids》2013,32(6):1233-1249
Abstract Some carbocyclic uridines and cytidines have been prepared in a palladium-catalyzed reaction between 5-substituted uracils and cytosines and diacetoxymethylcyclopentene, prepared in a Prins reaction. The antiviral activity of the nucleoside analogues have been tested. 相似文献
103.
Norma L. Yoss Octavian Popescu Victor I. Pop Dorin Porutiu Fred A. Kummerow Gheorghe Benga 《Bioscience reports》1985,5(1):1-5
Methods for the complete characterization of liposomes prepared by ether-injection are described in detail. The validity of atomic absorption spectrophotometry Ior measuring markers of trapped volume was checked by comparative determinations of markers with established optical spectrophotometrical methods. The favorable results usingl atomic absorption spectrophotometry to quantitate the marker Mn2+ are of particular relevance as manganese ion is also the paramagnetic probe in n.m.r, measurements of water permeability of lipo-somes; our results indicate that in such measurements no other marker need be incorporated. 相似文献
104.
A comparison of water diffusion in human erythrocytes and ghosts revealed a longer relaxation time in ghosts, A comparison of water diffusion in human erythrocytes and ghosts revealed a longer relaxation time in ghosts, corresponding to a decreased exchange rate. However, the diffusional permeability of ghosts was not significantly different from that of erythrocytes . The changes in water diffusion following exposure to p-chloromercuribenzene sulfonate (PCMBS) have been studied on ghosts suspended in isotonic solutions. It was found that a significant inhibitory effect of PCMBS on water diffusion occurred only after several minutes of incubation at 37°C. No inhibition was noticed after short incubation at 0°C as previously used in some labelling experiments. This indicates the location in the membrane interior of the SH groups involved in water diffusion across human erythrocyte membranes. The nuclear magnetic resonance ( n . m . r . ) method appears as a useful tool for studying changes in water diffusiofl in erythrocyte ghosts with the aim of locating the water channel. 相似文献
105.
Using the National Program for Sow Herd Management, two hypoprolific boars have been identified. The first one, a Large White, had produced on an average 7.2 piglets per litter in 24 litters. The second one, a Pietrain, had produced 6.11 piglets per litter in 28 litters. These animals are carrying two new chromosome translocations involving chromosomes 1 and 15 and 4 and 15, respectively. The animal production consequences of the chromosome translocations are discussed. 相似文献
106.
V. A. Popescu 《Plasmonics (Norwell, Mass.)》2018,13(5):1507-1511
Three recently published plasmonic biosensors based on a birefringent solid-core or a partial-solid-core microstructured optical fiber are simulated for detection of hemoglobin concentration in human blood. For a larger value of the number of holes n h but for the same value of the gold radius, the resonance spectral width and the difference between maximal amplitude sensitivity and resonant wavelengths are decreased, when the refractive index of the analyte is n a = 1.357. Also, the loss and maximum of the amplitude sensitivity are increased in the same conditions. At the resonant wavelength λ = 0.6496 μm for the devices with n h = 14, 17, and 35 holes and n a = 1.357, the hemoglobin concentration is close to the mean value (157.5 g/l) of a man. 相似文献
107.
The warm-season perennial switchgrass (Panicum virgatum) is a candidate bioenergy crop. To be successful, switchgrass production must be maintained on low-quality landscapes with minimal inputs while facing future climates that are expected to be more extreme and more variable. We propose that antecedent rainfall constrains how plants respond to drought, as well as subsequently recover from drought. To test this idea, we examined how six switchgrass genotypes responded to a 1-year severe drought and then recovered under normal rainfall in the following year. These plants had previously grown for 3 years under a range of dry to wet rainfall levels in a shallow-soil common garden with no fertilizer. Plants previously exposed to drought produced less biomass, and basal area after the severe drought was relieved compared to previously well-watered plants. In addition, there were legacy effects caused by plant size: plants that were larger pre-drought were more likely to survive the severe drought, and plants that were larger during the severe drought recovered more biomass, basal area, and tillers post-drought. Although genotypes differed somewhat in their responses, the size constraint was consistent across genotypes. These findings suggest that we can establish more drought-resilient switchgrass stands by, for example, planning for initial irrigation or planting during a wet year to allow plants to grow larger prior to experiencing drought. Additional studies are needed to understand whether these rainfall and size legacies persist or are transient. 相似文献
108.
William J. Kuhns Octavian Popescu Max M. Burger Gradimir Misevic 《Journal of cellular biochemistry》1995,57(1):71-89
Sulfate is an important component relating to normal proteoglycan secretion and normal motility in the marine sponge, Microciona prolifera. The following alterations were observed in sponge cells when sulfate free artificial sea water was used as the suspension medium: (1) impairment of aggregation, (2) loss of cell movements, (3) a marked reduction in the secretion of the adhesion proteoglycan (AP). Reversal of this effect occurred if sulfate depleted cells were again rotated in sulfate containing artificial sea water. Motility and reaggregation of sulfate deprived cells could be completely restored by purified AP, but only if cells were first pre-conditioned in normal sea water. Comparisons of 35SO42? uptake between normal and sulfate deprived cells which had been treated to reduce preformed secretions showed a marked increase in 35SO42? uptake and incorporation which could be greatly augmented in the presence of Ca2+/Mg2+. Excessive retention of AP in sulfate starved cells demonstrated by immunostaining suggested that AP secretion and cellular motility may be controlled by a sulfate dependent secretogogue or that undersulfated AP itself had developed a secretory defect. SDS-PAGE of Triton treated cellular extracts demonstrated a 116 kDa 35SO42? sulfated band which co-migrated with AP, but only in extracts derived from sulfate starved cells. Western blots prepared from such extracts incubated in the presence of a monoclonal anti-band 3 antibody demonstrated labelling of a single 97 kDa band only in material from sulfate deprived cells. The absence of this component in normal cell extracts indicated that this protein may be involved in facilitated sulfate transport. This study lends support to a heretofore unrecognized role for sulfate in cell motility and secretion. 相似文献
109.
C?t?lina Roxana Bohotin Magda B?descu D N Popescu V Bohotin 《Romanian journal of physiology》2004,41(1-2):99-108
Neurophysiologic, neuroanatomic and neuroimaging studies conducted over the past two decades reveal that the cerebral cortex is functionally and structurally dynamic. The functional topography of the motor cortex can be modified by a variety of experimental manipulations, including peripheral or central injury, electrical stimulation, pharmocologic treatment or behavioral experience. Recent evidence demonstrates that functional alterations in motor cortex organization are accompanied by changes in dendritic and synaptic structure, as well as alterations in the regulation of cortical neurotransmitter systems. This article describes the state of the science regarding the main mechanisms implicated in the motor cortex plasticity, the main tools used for its investigation and the consequence of the recent discoveries on the therapeutic and rehabilitation procedures for the brain-injured persons. 相似文献
110.
Abhishek Juluri Carmen Popescu Leon Zhou Reena N. Murthy Vanaja K. Gowda Chetan Kumar P Manjeet B. Pimparade Michael A. Repka S. Narasimha Murthy 《AAPS PharmSciTech》2016,17(1):99-105
The objective of this project was to investigate the potential of Kleptose Linecaps DE17 (KLD) in masking the unpleasant/bitter taste of therapeutic agents by hot melt extrusion (HME). Griseofulvin (GRI) and caffeine anhydrous (CA) were used as a bitter active pharmaceutical ingredient (API) model drugs. Thermogravimetric studies confirmed the stability of GRI, CA, and KLD at the employed extrusion temperatures. The differential scanning calorimetry (DSC) studies revealed a characteristic melting endotherm of GRI at 218–220°C and CA at 230–232°C in the physical mixtures as well as in all extrudates over the period of study, indicating the crystalline nature of drug. HME of KLD was achieved only in the presence of plasticizer. Among the several plasticizers investigated, xylitol showed improved processability of KLD at 15% w/w concentration. Dissolution studies of HME extrudates using simulated salivary medium exhibited ~threefold less release compared to physical mixture at the end of 5 min (the lesser drug release, better the taste masking efficiency). Furthermore, the results from the sensory evaluation of products in human panel demonstrated strong bitter taste in the case of physical mixture compared to the HME formulation, suggesting the potential of Kleptose Linecaps DE17 as taste masking polymer in melt extruded form. 相似文献