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61.
Effect of heparin on the stimulation of non-vascular cells by human acidic and basic FGF 总被引:8,自引:0,他引:8
S Uhlrich O Lagente M Lenfant Y Courtois 《Biochemical and biophysical research communications》1986,137(3):1205-1213
We have purified acidic and basic fibroblast growth factors from human brain (h-aFGF, h-bFGF) and studied the effect of heparin on the growth stimulation by these factors of hamster fibroblast CC139 cells and bovine epithelial lens (BEL) cells. In both the presence and the absence of foetal calf serum (FCS) heparin cooperates with h-aFGF in a dose dependent manner to stimulate both types of cells. The cooperation with h-bFGF is much less. An unpurified human brain fraction containing both factors behaves differently: in the absence of FCS, heparin enhances the activity of the crude fraction on BEL cells, while in the presence of FCS, it decreases this activity. These results indicate that heparin cooperates strongly with h-aFGF to stimulate non-vascular cell proliferation while in a partially purified extract and in the presence of serum it can induce the opposite effect. 相似文献
62.
When chick lens epithelium is cultured in vitro, differentiation into lens fiber cells is accompanied by DNA degradation. This phenomenom of terminal differentiation was studied in the epithelium from embryos at the 6th and 11th days of development. DNA size and the ability of the cells to repair DNA damage induced by X-rays were analysed in alkaline sucrose gradients. In the 6-day epithelium a rapid degradation and complete lack of DNA repair were recorded. Similar observations have been made in previous studies on the 11-day sample, but here degradation is progressive and occurs after a lag of several days. In the younger epithelium, internal irradiation by [3H]thymidine also had a drastic effect resembling that caused by X-rays. In order to assess the process of differentiation in our experimental system the synthesis of delta- and alpha-crystallins was monitored. Stage-related modifications in the rates of synthesis were recorded. The results confirm that the DNA repair system is impaired during terminal differentiation. The differences observed between the two stages may reflect either a developmental modification in DNA repair mechanisms or a change in the relative proportions of differentiating cells. An hypothesis is proposed in support of the latter case. 相似文献
63.
64.
This article summarizes some of the data that have been accumulated on several growth factors. Biochemical and biological properties of the Epidermal, Fibroblast, Astrocytes and Tumor growth factors (EGF, FGF, AGF, TGF) and those of growth factors derived from Platelets (PDGF), Brain (BDGF, ECGF), Eye (EDGF) and Cartilage (CDGF) are reviewed, as well as the in vitro mechanism of action of EGF and PDGF. The in vivo effects of these growth factors, particularly the experiments achieved to understand the physiological or physiopathological significance are described. The potential interest of these molecules in pharmacology and their use as wound healing agents is discussed. 相似文献
65.
M F Counis E Chaudun Y Courtois C J Skidmore 《Biochemical and biophysical research communications》1985,126(2):859-866
Nuclear ADP-ribosyltransferase is present in cells from the chick lens throughout embryonic development. The activity does not decrease when the cells become post-mitotic and commence terminal differentiation but declines slowly in both epithelia and fibre cells. At all stages studied the enzyme retains its ability to be activated by DNA strand breaks induced either by X-irradiation or by the action of an endogenous endonuclease. There is no correlation between the enzyme activity or the levels of its substrate NAD+ and the changes in DNA repair capacity which have been observed during the development of the lens. 相似文献
66.
67.
Germain Montazeaud Cyrille Violle Hélène Fréville Delphine Luquet Nourollah Ahmadi Brigitte Courtois Ilyas Bouhaba Florian Fort 《Plant and Soil》2018,429(1-2):187-198
Aims
A growing body of research supports the feasibility of biocrust rehabilitation. Identifying populations of key species that are amenable to cultivation and that are resilient in rehabilitation contexts would advance the efficacy of these technologies. Here we investigate the growth and stress response of the cosmopolitan biocrust moss, Syntrichia ruralis.Methods
We sampled populations of S. ruralis along a precipitation seasonality gradient from the Colorado Plateau ecoregion of the western United States. We cultivated these populations in an experiment manipulating duration of hydration periods on a weekly cycle. We then treated greenhouse grown materials with brief, stressful watering events, measuring how many events they could survive.Results
All populations grew at an accelerated rate compared to growth in a natural setting, at least doubling biomass in five months. Increasing duration of hydration periods led to more growth in all but one population. Volunteer biocrust algae and cyanobacteria developed during cultivation, and differed among populations. Greenhouse grown mosses differed in their response to stressful watering, with the most susceptible populations dying at half the number events compared to the most tolerant.Conclusions
These findings argue for informed selection and deployment of Syntrichia ruralis populations for soil rehabilitation.68.
David Castonguay Julien Dufort-Gervais Caroline Ménard Manavi Chatterjee Rémi Quirion Bruno Bontempi Jay S. Schneider Amy F.T. Arnsten Angus C. Nairn Christopher M. Norris Guylaine Ferland Erwan Bézard Pierrette Gaudreau Paul J. Lombroso Jonathan Brouillette 《Current biology : CB》2018,28(7):1079-1089.e4
69.
Exopolysaccharide and Poly-(beta)-Hydroxybutyrate Coproduction in Two Rhizobium meliloti Strains 下载免费PDF全文
P. Tavernier J. Portais Sa Nava J. Courtois B. Courtois J. Barbotin 《Applied microbiology》1997,63(1):21-26
The effects of different nitrogen and carbon sources on cell growth, pH, and exopolysaccharide (EPS) and poly-(beta)-hydroxybutyrate (PHB) production by two strains of Rhizobium meliloti (M5N1 and Su47) are reported. Differences in the behavior of glucose- and fructose-grown cells were shown, in particular with the M5N1 strain. Growth in a glucose-containing medium was accompanied by acidification of the culture medium, which leads to cell death. On fructose, acidification was detected only in the medium with a mineral nitrogen supply. A lag phase in EPS production was observed with cells grown with glucose, probably related to an initial extracellular conversion of the carbohydrate into an acid. No lag phase was observed in EPS production from fructose or in PHB synthesis whatever the carbon source. A decrease in PHB content was noticed for both strains under conditions where acidification of media occurred. The extent of production, emphasized by the use of a coproduction index, indicates that the M5N1 strain is a more promising organism than is the Su47 strain for polymer production. Such a strain, put in rich medium (containing yeast extract) supplemented with fructose, accumulated PHB up to 85% of dry cell weight and excreted about 1.5 g of EPS per liter in the medium. Regulation of the coproduction of EPS and PHB by these cells is suggested. 相似文献
70.
Mapping genes controlling root morphology and root distribution in a doubled-haploid population of rice 总被引:31,自引:0,他引:31
R. Yadav B. Courtois N. Huang G. McLaren 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(5):619-632
A deep thick root system has been demonstrated to have a positive effect on yield of upland rice under water stress conditions.
Molecular-marker-aided selection could be helpful for the improvement of root morphological traits, which are otherwise difficult
to score. We studied a doubled-haploid population of 105 lines derived from an indica×japonica cross and mapped the genes controlling root morphology and distribution (root thickness, maximum root length, total root
weight, deep root weight, deep root weight per tiller, and deep root to shoot ratio). Most putative QTL activity was concentrated
in fairly compact regions on chromosomes 1, 2, 3, 6, 7, 8 and 9, but was widely spread on chromosome 5 and largely absent
on chromosomes 4, 10, 11 and 12. Between three and six QTLs were identified on different chromosomes for each trait. Individual
QTLs accounted for between 4 and 22% of the variation in the traits. Multiple QTL models accounted for between 14 and 49%.
The main QTLs were common between traits, showing that it should be possible to modify several aspects of root morphology
simultaneously. There was evidence of interaction between marker locations in determining QTL expression. Interacting locations
were mostly on different chromosomes and showed antagonistic effects with magnitudes large enough to mask QTL detection. The
comparison of QTL locations with another population showed that one to three common QTLs per trait were recovered, among which
the most significant was in one or other population. These results will allow the derivation of isogenic lines introgressed
with these common segments, separately in the indica and japonica backgrounds.
Received: 12 August 1996 / Accepted: 15 November 1996 相似文献