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71.
近交系BALB/c小鼠及先天遗传性白内障突变型BALB/cBk-Cat小鼠的DNA指纹研究 总被引:1,自引:0,他引:1
采用人工合成的寡核苷酸探针(GATA)~4|分别与近交系小鼠BALB/c和经10年 30代培育而成的自发突变型近交系先天遗传性白内障BALB/cBk-Cat小鼠进行DNA分杂交,DNA指纹显示:(1)BALB/c小鼠个体之间以及BALB/cBk-C at小鼠个体之间没有差异;(2)BALB/c小鼠与BALB/cBk-Cat小鼠的DNA指纹在23-6kb之间的区域有差异。 相似文献
72.
小麦T型细胞质雄性不育系与相应保持系线粒体DNA的RAPD分析 总被引:14,自引:1,他引:13
黄占景 沈银柱 刘植义 何聪芬 柏峰 马闻师 姚鸿 王京兆 王斌HUANG Zhan-Jing SHEN Yin-Zhu LIU Zhi-Yi HE Cong-Fen BO Feng MA Wen-Shi YAO Hong WANG Jing-Zhao WANG Bin 《遗传》1997,19(4):8-11
利用RAPD技术对小麦T型细胞质雄性不育系75-3369A和相应保持系75-336 9B
的线粒体DNA(mtDNA)进行了81个单引物和14组双引物的多态性研究。结果有73个单引物和全部的双引物有扩增结果,表现出多态性的有10个单引物和一组双引物。
Abstract:Using 81 single-primers and 14 double-primers,T-type CMS(cytoplasmic male sterile)line 75-3369 A and is maintainer 75-3369B were studied by means of RAPD.73 single-primers and all double-primers gave amplified products in the experiment,among them 10 single-primers and one double-primer presented polymorphism. 相似文献
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74.
作为功能基因组学中重要的组成部分,基因表达谱在生物学、医学和药物研发等多个领域发挥着重要作用.特别是随着精准医疗概念的提出,整合多组学数据用于个性化医疗是未来的发展趋势.本文从基因表达谱的基本概念出发,重点介绍面向药物发现的基因表达谱分析方法,即基于关联图谱的方法、基于基因调控网络的方法和基于多组学数据整合的方法.系统整理了各种方法的研究进展,特别是在抗癌药物研发领域的最新进展,为利用基因表达谱数据进行药物研发提供方法借鉴. 相似文献
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77.
Biomass and production of phyto- and bacterio-plankton in eutrophic Lake Tystrup, Denmark 总被引:4,自引:0,他引:4
BO RIEMANN 《Freshwater Biology》1983,13(4):389-398
SUMMARY.
- 1 Phytoplankton primary production and biomass were compared with bacterial secondary production estimated by means of frequency of dividing cells (FDC) in eutrophic Lake Tystrup. Denmark.
- 2 In the upper part of the photic zone, bacterial secondary production constituted 12% of the carbon fixed by the phytoplankton. In vertical profiles, bacterial secondary production ranged from 7.6% (early spring) to 121% (during August) of the carbon fixed by the algae.
- 3 A close relationship was found between occurrence and activity of bacteria and algae, suggesting that released organic products are of primary importance to the bacteria.
- 4 The annual phytoplankton primary production was estimated as 227 g C m-2 compared to 102 g C m-2 assimilated by the bacteria, so 45% of the carbon fixed by the phytoplankton went through pelagic bacteria.
78.
Michael H. Beale John R. Bearder Jake Macmillan Akihiko Matsuo Bernard O. Phinney 《Phytochemistry》1983,22(4):875-881
The diterpene acid content in 10 species of Helianthus has been investigated. Ent-12,16-cyclokauranoic acid, isolated from H. annuus, is converted into a series of 12,16-cyclogibberellins by cultures of Gibberella fujikuroi, mutant B1-41a, and 12,16-cyclogibberellins A9, and A12 have been isolated. Ent-12β-acetoxykaurenoic acid and ent-13(S)-angeloxyatisenoic acid have been isolated from H. decapetalus; the metabolism of ent-13(S)-hydroxyatisenoic acid and atisenoic acid by B1-41a is also described. 相似文献
79.
When grown on PDL medium for 11 days the strain REC-193A of Gibberella fujikuroi produces the usual range of gibberellins and ent-kaurenoid metabolites. After 3–5 days under the same conditions of culture, this slow growing strain produces virtually none of these metabolites. These short term cultures were found to convert gibberellin A12-aldehyde into gibberellins A12 (8.3 % A14 (45%), A4 (ca. 17%) and A7 (ca. 6%). Under identical conditions of culture gibberellin A12 was largely unmetabolised. These results show that 3-hydroxylation is the first step in the conversion of gibberellin A12-aldehyde into gibberellins A14, A4 and A7. 相似文献
80.
Sampling of benthic algae from selected sites in the Metolius River was carried out at regular intervals between April 15, 1967 and March 9, 1968 to determine the species composition of the communities. With the exception of the sampling site at the headwaters and the site immediately downstream, the benthic community of the Metolius River is described as a Cladophora–Achnanthes–Spirogyra community, with species of these 3 genera predominating on a year-round basis. The community described for the headwaters and the sampling site immediately downstream was composed primarily of Nitzchia palea and Cymbella cistula. Of the approximately 60 species of algae which were identified, only 9 showed a definite seasonal distribution, the remaining species being found on a year-round basis. 相似文献