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51.
Petro J. Zapata M. Ángeles Botella M. Teresa Pretel Maria Serrano 《Plant Growth Regulation》2007,53(2):97-106
The effect of salinity (100 mM NaCl) on ethylene metabolism in the early phase of vegetative development of several plant
species has been investigated. The effects of saline treatment on shoot and root growth, ranged in sensitivity with respect
to species: pepper (Capsicum annum L. cv Pairal) > tomato (Lycopersicon esculentum Mill. cv Malpica) > broccoli (Brassica oleraceae L. var. Italica Plenk. cv Marathon F1) ≅ lettuce (Lactuca sativa var. longifolia Lam. cv Inverna) ≅ melon (Cucumis melo L. cv Ruano F1, Roche type) > bean (Phaseolus vulgaris L. cv. Gator Green 15) ≅ spinach (Spinacia oleracea L. cv Boeing) > beetroot (Beta vulgaris L. var. crassa (Alef.) J. Helm. cv Detroit). After saline treatment, ethylene production increased 4.2-fold in pepper shoots. Significant
increases were also found in shoots of tomato, broccoli and bean. In contrast, salinity decreased shoot ethylene production
rate in melon, spinach, and beetroot. In roots, the general effect of salinity was a decrease in ethylene production, especially
in broccoli and bean, except in tomato root, in which a sharp increase in ethylene production occurred. In general, saline
treatment increased total ACC concentration in both shoot and root in most of the plant species examined, which was related
to plant sensitivity to salinity. For example, pepper shoot was the most sensitive to saline treatment, showing the highest
fresh weight inhibition and the highest increase in total ACC concentration (8.5-fold), while, beetroot was less affected
by salinity and showed no effect on total ACC concentration in response to saline treatment. 相似文献
52.
Hilde Van Esch Rita Colnaghi Kathleen Freson Petro Starokadomskyy Andreas Zankl Liesbeth Backx Iga Abramowicz Emily Outwin Luis Rohena Claire Faulkner Gary M. Leong Ruth A. Newbury-Ecob Rachel C. Challis Katrin Õunap Jacques Jaeken Eve Seuntjens Koen Devriendt Ezra Burstein Mark O’Driscoll 《American journal of human genetics》2019,104(5):957-967
53.
Rudolf Klemetti Katherine M. Steele Petro Moilanen Janne Avela Jussi Timonen 《Journal of biomechanics》2014
This study was conducted to analyze the unimpaired control of the trunk during walking. Studying the unimpaired control of the trunk reveals characteristics of good control. These characteristics can be pursued in the rehabilitation of impaired control. Impaired control of the trunk during walking is associated with aging and many movement disorders. This is a concern as it is considered to increase fall risk. Muscles that contribute to the trunk control in normal walking may also contribute to it under perturbation circumstances, attempting to prevent an impending fall. Knowledge of such muscles can be used to rehabilitate impaired control of the trunk. Here, angular accelerations of the trunk induced by individual muscles, in the sagittal and frontal planes, were calculated using 3D muscle-driven simulations of seven young healthy subjects walking at free speed. Analysis of the simulations demonstrated that the abdominal and back muscles displayed large contributions throughout the gait cycle both in the sagittal and frontal planes. Proximal lower-limb muscles contributed more than distal muscles in the sagittal plane, while both proximal and distal muscles showed large contributions in the frontal plane. Along with the stance-limb muscles, the swing-limb muscles also exhibited considerable contribution. The gluteus medius was found to be an important individual frontal-plane control muscle; enhancing its function in pathologies could ameliorate gait by attenuating trunk sway. In addition, since gravity appreciably accelerated the trunk in the frontal plane, it may engender excessive trunk sway in pathologies. 相似文献
54.
Intracellular localization of the p35 subunit of murine IL-12 总被引:2,自引:0,他引:2
Production of interleukin-12 (IL-12), a heterodimer of p35 and p40 subunits, is limited by p35 expression. A long and a short murine p35 mRNA potentially encoding proteins differing in pre-sequence size are produced. Increased pre-sequence size could convert a cleaved signal peptide to an uncleaved signal peptide, raising the possibility that a membrane-bound form of p35 is produced. The intracellular localization of the p35 encoded by each mRNA isoform was determined by constructing cDNAs containing the long or short p35 cDNA isoform fused in-frame to a cDNA encoding green fluorescent protein (GFP). After transfection of a CV-1 African green monkey kidney cell line with the constructs, confocal microscopy and immunoblotting of extracted microsomal membranes demonstrated that the p35-GFP fusion protein encoded by the long or short mRNA accumulates in the Golgi apparatus as an endoglycosidase H-sensitive glycosylated integral membrane protein. In contrast, a p40-GFP fusion protein accumulates in the Golgi apparatus as a soluble protein. Since assembly of the p35 and p40 subunits to form bioactive IL-12 occurs in the ER, release of membrane-tethered IL-12 by proteolytic cleavage in a late Golgi or post-Golgi compartment may represent an as yet unidentified level at which bioactive IL-12 secretion is regulated. 相似文献
55.
Background
Lyme disease, caused by Borrelia burgdorferi, affects a large number of people in both the USA and Europe. The mouse is a natural host for this spirochete and is widely used as a model system to study Lyme pathogenesis mechanisms. Since disease manifestations often depend upon the spirochete burden in a particular tissue, it is critical to accurately measure the bacterial number in infected tissues. The current methods either lack sensitivity and specificity (SYBR Green), or require independent analysis of samples in parallel to quantitate host and bacterial DNA (TaqMan). We have developed a novel molecular beacon-based convenient multiplex real-time quantitative PCR assay to identify and detect small numbers of B. burgdorferi in infected mouse tissues. 相似文献56.
57.
Comparison of rotavirus strains by hemagglutination inhibition. 总被引:2,自引:0,他引:2
Rotaviruses have been shown to be of importance as aetiologic agents of gastroenteritis in infants and in domestic animals of several species. Hemagglutinins were prepared from two Canadian isolates of bovine rotavirus and from one isolate of a simian rotavirus. A United Kingdon isolate of bovine rotavirus was shown not to possess hemagglutinating activity, indicating a strain difference between a Canadian and United Kingdom bovine rotavirus. In hemagglutination-inhibition (HAI) tests a rabbit hyperimmune (two injections) serum, prepared to one of the bovine rotaviruses, was not helpful in distinguishing the two bovine viruses because of cross-reactions between the viruses. However, it was possible to distinguish the bovine viruses from the simian virus with this serum. When guinea pig immune sera were prepared to the four rotavirus strains and tested with the three hemagglutinins in the HAI test, antigenic differences between the four strains of rotavirus were demonstrated. Hyperimmune guinea pig serum prepared to a strain of human rotavirus did not inhibit any of three hemagglutinins indicating that the human strain is different from the three rotavirus strains which gave hemagglutinins. 相似文献
58.
59.
An ATPase activity specifically stimulated by micromolar Ca2+ concentrations has been identified in association with rabbit neurophil membranes. These studies provide the basis of further characterization of the Ca2+-ATPase activity with regard to neutrophil function. 相似文献
60.