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41.
Shoots of Centaurium erythraea Rafn were cultivated in 5 l mist trickling bioreactor for 21 and 28 days increasing their dry weight from 0.54 g to 13.7 g and 18.3 g, respectively. About 6880 shoots from 223 initial shoot-tips in 21-day bioreactor producing cycle were produced. The shoots could be successfully rooted and transferred to soil. Secoiridoid accumulation (expressed as a sum of gentiopicroside, sweroside and swertiamarin) in shoots after 21 days of culture reached about 303 mg l−1. 相似文献
42.
Kocharova NA Maszewska A Zatonsky GV Torzewska A Bystrova OV Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2004,339(2):415-419
Studies of the O-polysaccharide chain of the lipopolysaccharide (O-antigen) of Providencia alcalifaciens O19 by sugar and methylation analyses along with NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, NOESY and 1H,13C HSQC experiments, showed that the pentasaccharide repeating unit of the polysaccharide has the following structure: [structure: see text] where Fuc3NAc is 3-acetamido-3,6-dideoxygalactose. The unique structure of the O-antigen and serological data are in consistence with classification of this bacterium in a separate Providencia serogroup. 相似文献
43.
44.
Bonar E Dubin A Bierczyńska-Krzysik A Noga M Silberring J Stalińska K Koj A 《Cytokine》2006,33(2):111-117
Interleukin-1 (IL-1) and interleukin-6 (IL-6) are principal proinflammatory cytokines inducing the acute phase response of various tissues, including liver. Cultured human hepatoma HepG2 cells were stimulated with IL-1 (10 ng/ml) and IL-6 (10 ng/ml). After 24 h the cells were collected and disrupted by sonication in a lysis buffer containing 8M urea. The extracted cellular proteins were separated by 2D polyacrylamide gel electrophoresis. The gels were stained with Coomassie Brilliant Blue R-250 and the protein spots showing different intensities in comparison to control (unstimulated) cells were excised and subjected to analysis by LC-MS/MS. Alternatively, proteins were stained with SYPRO Ruby. These differentially expressed proteins include seven up-regulated and two down-regulated intracellular proteins of various functions. The identification of three cytokine-responsive proteins was confirmed by biosynthetic labeling with [35S]methionine after incubation of HepG2 cells, and by western blot with specific antisera. 相似文献
45.
Stanisław Szala Iwona Mitrus Aleksander Sochanik 《Cancer immunology, immunotherapy : CII》2010,59(10):1449-1455
Cancer initiation and progression is strongly influenced by the tumor microenvironment consisting of various types of host
cells (inflammatory cells, vascular cells and fibroblasts), extracellular matrix and non-matrix molecules. Host cells play
a defining role in two major processes crucial for tumor growth: angiogenesis and escape from immune surveillance. The interdependence
of these processes resemble the principles of Yin and Yang, as the stimulation of tumor angiogenesis inhibits effective immune
responses, while angiogenesis inhibition may have the opposite effect. These considerations may be useful in developing anticancer
strategies based on the potentially synergistic combinations of antiangiogenic and immunostimulatory drugs. 相似文献
46.
47.
Baby G. Tholanikunnel Kusumam Joseph Karthikeyan Kandasamy Aleksander Baldys John R. Raymond Louis M. Luttrell Paul J. McDermott Daniel J. Fernandes 《The Journal of biological chemistry》2010,285(44):33816-33825
β2-Adrenergic receptors (β2-AR) are low abundance, integral membrane proteins that mediate the effects of catecholamines at the cell surface. Whereas the processes governing desensitization of activated β2-ARs and their subsequent removal from the cell surface have been characterized in considerable detail, little is known about the mechanisms controlling trafficking of neo-synthesized receptors to the cell surface. Since the discovery of the signal peptide, the targeting of the integral membrane proteins to plasma membrane has been thought to be determined by structural features of the amino acid sequence alone. Here we report that localization of translationally silenced β2-AR mRNA to the peripheral cytoplasmic regions is critical for receptor localization to the plasma membrane. β2-AR mRNA is recognized by the nucleocytoplasmic shuttling RNA-binding protein HuR, which silences translational initiation while chaperoning the mRNA-protein complex to the cell periphery. When HuR expression is down-regulated, β2-AR mRNA translation is initiated prematurely in perinuclear polyribosomes, leading to overproduction of receptors but defective trafficking to the plasma membrane. Our results underscore the importance of the spatiotemporal relationship between β2-AR mRNA localization, translation, and trafficking to the plasma membrane, and establish a novel mechanism whereby G protein-coupled receptor (GPCR) responsiveness is regulated by RNA-based signals. 相似文献
48.
Hinzpeter A Lipecka J Brouillard F Baudoin-Legros M Dadlez M Edelman A Fritsch J 《American journal of physiology. Cell physiology》2006,290(1):C45-C56
The voltage-dependent ClC-2 chloride channel has been implicated in a variety of physiological functions, including fluid transport across specific epithelia. ClC-2 is activated by hyperpolarization, weakly acidic external pH, intracellular Cl, and cell swelling. To add more insight into the mechanisms involved in ClC-2 regulation, we searched for associated proteins that may influence ClC-2 activity. With the use of immunoprecipitation of ClC-2 from human embryonic kidney-293 cells stably expressing the channel, followed by electrophoretic separation of coimmunoprecipitated proteins and mass spectrometry identification, Hsp70 and Hsp90 were unmasked as possible ClC-2 interacting partners. Association of Hsp90 with ClC-2 was confirmed in mouse brain. Inhibition of Hsp90 by two specific inhibitors, geldanamycin or radicicol, did not affect total amounts of ClC-2 but did reduce plasma membrane channel abundance. Functional experiments using the whole cell configuration of the patch-clamp technique showed that inhibition of Hsp90 reduced ClC-2 current amplitude and impaired the intracellular Cl concentration [Cl]-dependent rightward shift of the fractional conductance. Geldanamycin and radicicol increased both the slow and fast activation time constants in a chloride-dependent manner. Heat shock treatment had the opposite effect. These results indicate that association of Hsp90 with ClC-2 results in greater channel activity due to increased cell surface channel expression, facilitation of channel opening, and enhanced channel sensitivity to intracellular [Cl]. This association may have important pathophysiological consequences, enabling increased ClC-2 activity in response to cellular stresses such as elevated temperature, ischemia, or oxidative reagents. heat shock; geldanamycin; cellular stress; channel trafficking; transepithelial chloride transport 相似文献
49.
Kavdia M Popel AS 《American journal of physiology. Heart and circulatory physiology》2006,290(2):H716-H723
Venular endothelial cells can release nitric oxide (NO) in response to intraluminal flow both in isolated venules and in vivo. Experimental studies suggest that venular endothelium-released NO causes dilation of the adjacent paired arteriole. In the vascular wall, NO stimulates its target hemoprotein, soluble guanylate cyclase (sGC), which relaxes smooth muscle cells. In this study, a computational model of NO transport for an arteriole and venule pair was developed to determine the importance of the venular endothelium-released NO and its transport to the adjacent arteriole in the tissue. The model predicts that the tissue NO levels are affected within a wide range of parameters, including NO-red blood cell reaction rate and NO production rate in the arteriole and venule. The results predict that changes in the venular NO production affected not only venular endothelial and smooth muscle NO concentration but also endothelial and smooth muscle NO concentration in the adjacent arteriole. This suggests that the anatomy of microvascular tissue can permit the transport of NO from arteriolar to venular side, and vice versa, and may provide a mechanism for dilation of proximal arterioles by venules. These results will have significant implications for our understanding of tissue NO levels in both physiological and pathophysiological conditions. 相似文献
50.
Kołodziej Barbara Kochan Ewa Kazimierczak Jerzy Chmiel Aleksander 《Plant Growth Regulation》2006,48(1):13-19
American ginseng (Panax quinquefolium L.) is a perennial medicinal herb originally grown in Canada and USA, and recently also in China, Australia, Holland and
Poland. Several commercial preparations are produced from ginseng roots, that are known for their antifatigue, antitumor,
antistress and immune system stimulating functions. The medicinal properties are due mainly to the active components – ginsenosides.
In this work, the results of field cultivation experiments are presented that examine the effects of foliar application of
several growth regulators on quality parameters and ginsenoside content of P. quinuefolium roots. The growth regulators tested, i.e., kinetin, daminozide, mixture of gibberellic acid (GA3) with potassium salt of α-naphthalene acetic acid (kNAA) and new preparation – IPO-1 – benzimidazole derivative (obtained
from the Institute of Organic Industry in Warsaw – at present during the process of patent), were applied at a concentration
of 100 or 200 mg l−1 in the middle of June in the 2nd year of vegetation. After 4 years of cultivation, the roots were dug up and dried, and subsequently
the quantitative analysis of individual saponins (Rb1, Rb2, Rc, Rd, Re, Rg1) by HPLC was performed. Growth regulators significantly affected quality parameters, morphological features and accumulation
of individual and total ginsenosides in ginseng roots. Regardless of doses, the plant roots treated with growth regulators
had a higher content of total ginsenosides in comparison to the control. The growth regulators also affected individual ginsenosides
level and narrowed the ratio of Rb:Rg group. The application of kinetin, daminozide and benzimidazole derivative for foliar spray during 2nd year of American ginseng
vegetation caused a significant increase in air dry weight of roots and aboveground parts whereas the mixture of GA3 and kNAA showed a decreasing effect. An increase of roots size was observed using higher doses (200 mg l−1) of kinetin and daminozide while a decreasing tendency appeared with the application of the other preparations. 相似文献